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ppa inorganic pyrophosphatase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948748, updated on 23-Nov-2023

Summary

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Gene symbol
ppa
Gene description
inorganic pyrophosphatase
Primary source
ASAP:ABE-0013824
Locus tag
b4226
See related
ECOCYC:EG10755
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK4222
Summary
The enzyme is a hexamer, arranged as a dimer of trimers in the crystal structure . [More information is available at EcoCyc: EG10755].
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Genomic context

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Sequence:
NC_000913.3 (4449122..4449652, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene gamma-glutamylamine cyclotransferase family protein YtfP Neighboring gene ChpS antitoxin of the ChpB-ChpS toxin-antitoxin system Neighboring gene endoribonuclease toxin ChpB Neighboring gene galactofuranose ABC transporter periplasmic binding protein Neighboring gene galactofuranose ABC transporter putative ATP binding subunit

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Constant enthalpy change value during pyrophosphate hydrolysis within the physiological limits of NaCl. Wakai S, et al. J Biol Chem, 2013 Oct 11. PMID 23965994, Free PMC Article
  2. DFT study on the mechanism of Escherichia coli inorganic pyrophosphatase. Yang L, et al. J Phys Chem B, 2009 May 7. PMID 19366250
  3. Reversible inhibition of Escherichia coli inorganic pyrophosphatase by fluoride: trapped catalytic intermediates in cryo-crystallographic studies. Samygina VR, et al. J Mol Biol, 2007 Mar 2. PMID 17196979
  4. Metal-free PPi activates hydrolysis of MgPPi by an Escherichia coli inorganic pyrophosphatase. Vainonen JP, et al. Biochemistry (Mosc), 2005 Jan. PMID 15701051
  5. Cd(2+)-induced aggregation of Escherichia coli pyrophosphatase. Zimenkov YV, et al. Eur J Biochem, 2004 Jul. PMID 15233803

See all (65) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Data indicate that the pyrophosphatase activities of non-halophilic Escherichia coli was 0.1 M NaCl.
    Title: Constant enthalpy change value during pyrophosphate hydrolysis within the physiological limits of NaCl.
  2. N-terminus verified by Edman degradation on mature peptide
    Title: '98 Escherichia coli SWISS-2DPAGE database update.
  3. We further determined the crystal structure of the Arg43Gln mutant variant of E-PPase complexed with one phosphate and four Mn ions.
    Title: Reversible inhibition of Escherichia coli inorganic pyrophosphatase by fluoride: trapped catalytic intermediates in cryo-crystallographic studies.
  4. Results demonstrate that the flexibility of the loops where the residues Gly100 and Gly147 are located is necessary at the stages of substrate binding and product release.
    Title: Substitutions of glycine residues Gly100 and Gly147 in conservative loops decrease rates of conformational rearrangements of Escherichia coli inorganic pyrophosphatase.
  5. Results show that the substitutions of Gly100 and Gly147 result in overall destabilization of the globular structure.
    Title: Effect of mutation of the conservative glycine residues Gly100 and Gly147 on stability of Escherichia coli inorganic pyrophosphatase.
  6. Results report a novel human protein, calphoglin, which activates inorganic pyrophosphatase (IPP) and enhances phosphoglucomutase activity through the activated IPP.
    Title: Cellular signaling mediated by calphoglin-induced activation of IPP and PGM.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables inorganic diphosphate phosphatase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables inorganic diphosphate phosphatase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables inorganic diphosphate phosphatase activity IEA
Inferred from Electronic Annotation
more info
  
enables inorganic triphosphate phosphatase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables magnesium ion binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables magnesium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables magnesium ion binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables zinc ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in phosphate-containing compound metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in phosphate-containing compound metabolic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol HDA PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane HDA PubMed 

General protein information

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Preferred Names
inorganic pyrophosphatase
NP_418647.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4449122..4449652 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418647.1 inorganic pyrophosphatase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418647.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A775TFT7
    Conserved Domains (1) summary
    PRK01250
    Location:1 → 176
    PRK01250; inorganic diphosphatase
Nucleotide Protein
Heading Accession and Version

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