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tsr methyl-accepting chemotaxis protein Tsr [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948884, updated on 11-Apr-2024

Summary

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Gene symbol
tsr
Gene description
methyl-accepting chemotaxis protein Tsr
Primary source
ASAP:ABE-0014282
Locus tag
b4355
See related
ECOCYC:EG11034
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
cheD; ECK4345
Summary
Tsr also senses energy levels and is involved in aerotaxis, redox taxis and glycerol repellent taxis. [More information is available at EcoGene: EG11034]. Tsr is a serine chemoreceptor in Escherichia coli and binds directly to the MCP periplasmic domain. [More information is available at EcoCyc: EG11034].
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Genomic context

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Sequence:
NC_000913.3 (4591657..4593312)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene PF04328 family protein YjiX Neighboring gene pyruvate:H(+) symporter Neighboring gene galactonate:H(+) symporter Neighboring gene putative DNA-binding transcriptional regulator LgoR

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Structural signatures of Escherichia coli chemoreceptor signaling states revealed by cellular crosslinking. Flack CE, et al. Proc Natl Acad Sci U S A, 2022 Jul 12. PMID 35787052, Free PMC Article
  2. In Situ Conformational Changes of the Escherichia coli Serine Chemoreceptor in Different Signaling States. Yang W, et al. mBio, 2019 Jul 2. PMID 31266867, Free PMC Article
  3. Mutational Replacements at the "Glycine Hinge" of the Escherichia coli Chemoreceptor Tsr Support a Signaling Role for the C-Helix Residue. Pedetta A, et al. Biochemistry, 2017 Jul 25. PMID 28664727
  4. Signaling Consequences of Structural Lesions that Alter the Stability of Chemoreceptor Trimers of Dimers. Lai RZ, et al. J Mol Biol, 2017 Mar 24. PMID 28215934, Free PMC Article
  5. Tsr Chemoreceptor Interacts With IL-8 Provoking E. coli Transmigration Across Human Lung Epithelial Cells. Han B, et al. Sci Rep, 2016 Aug 10. PMID 27506372, Free PMC Article

See all (120) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Structural signatures of Escherichia coli chemoreceptor signaling states revealed by cellular crosslinking.
    Title: Structural signatures of Escherichia coli chemoreceptor signaling states revealed by cellular crosslinking.
  2. The authors show that individual Tsr dimers within a trimer exhibit asymmetric flexibilities that are a function of the signaling state, highlighting the effect of their different protein interactions at the receptor tips.
    Title: In Situ Conformational Changes of the Escherichia coli Serine Chemoreceptor in Different Signaling States.
  3. Blue light is a universal signal for Escherichia coli chemoreceptors.
    Title: Blue Light Is a Universal Signal for Escherichia coli Chemoreceptors.
  4. this study shows that Tsr interacts with IL-8 provoking E. coli transmigration across human lung epithelial cells
    Title: Tsr Chemoreceptor Interacts With IL-8 Provoking E. coli Transmigration Across Human Lung Epithelial Cells.
  5. results indicate that, rather than being essential for proper receptor-receptor interaction, the "glycine hinge" residues are involved in the ability of the receptor to switch between different signaling states. Mainly, the C-helix residue G439 has a key role in shifting the equilibrium toward a kinase-activating conformation.
    Title: Mutational Replacements at the "Glycine Hinge" of the Escherichia coli Chemoreceptor Tsr Support a Signaling Role for the C-Helix Residue.
  6. These results indicate that the E402 and R404 residues of Tsr play their most critical signaling roles at their inner locations near the trimer axis where they likely participate in stabilizing the trimer-of-dimer packing and the kinase-ON state of core signaling complexes.
    Title: Signaling Consequences of Structural Lesions that Alter the Stability of Chemoreceptor Trimers of Dimers.
  7. The authors suggest that the Tsr control cable transmits input signals to a four-helix HAMP bundle by modulating the intensity of structural clashes between out-of-register transmembrane helix and AS1 helix of HAMP.
    Title: Evidence for a Helix-Clutch Mechanism of Transmembrane Signaling in a Bacterial Chemoreceptor.
  8. Only strains that produce TynA and FeaB converting norepinephrine to 3,4-dihydroxymandelic acid xhibited chemotaxis toward norepinephrine and 3,4-dihydroxymandelic acid is sensed by the serine chemoreceptor Tsr.
    Title: Chemotaxis of Escherichia coli to norepinephrine (NE) requires conversion of NE to 3,4-dihydroxymandelic acid.
  9. Phe396 governs conformational changes of tsr.
    Title: A phenylalanine rotameric switch for signal-state control in bacterial chemoreceptors.
  10. Co-overexpression of Tsr, CheA, and CheW yields hexagonal Arrays.
    Title: New insights into bacterial chemoreceptor array structure and assembly from electron cryotomography.
Submit: New GeneRIF Correction See all GeneRIFs (31)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables transmembrane signaling receptor activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables transmembrane signaling receptor activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in cell motility IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in cellular response to amino acid stimulus IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in chemotaxis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in chemotaxis IEA
Inferred from Electronic Annotation
more info
  
involved_in chemotaxis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in detection of chemical stimulus IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in receptor clustering IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of bacterial-type flagellum-dependent cell motility IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of chemotaxis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of protein histidine kinase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in signal complex assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in signal transduction IEA
Inferred from Electronic Annotation
more info
  
involved_in signal transduction IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
part_of methyl accepting chemotaxis protein complex IMP
Inferred from Mutant Phenotype
more info
 PubMed 
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane IMP
Inferred from Mutant Phenotype
more info
 PubMed 

General protein information

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Preferred Names
methyl-accepting chemotaxis protein Tsr

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4591657..4593312
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418775.1 methyl-accepting chemotaxis protein Tsr [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418775.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8S7EFF2
    Conserved Domains (1) summary
    PRK15041
    Location:1 → 551
    PRK15041; methyl-accepting chemotaxis protein
Nucleotide Protein
Heading Accession and Version

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Other Literature Sources
Molecular Biology Databases
Research Materials