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thyA thymidylate synthase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 949035, updated on 4-Apr-2024

Summary

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Gene symbol
thyA
Gene description
thymidylate synthase
Primary source
ASAP:ABE-0009268
Locus tag
b2827
See related
ECOCYC:EG11002
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2823
Summary
Thymidylate synthase (TS) catalytic mechanism order: [More information is available at EcoGene: EG11002]. Thymidylate synthase plays a key role in DNA synthesis. [More information is available at EcoCyc: EG11002].
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Genomic context

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Sequence:
NC_000913.3 (2964361..2965155, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene prepilin-type N-terminal cleavage/methylation domain-containing protein PpdC Neighboring gene DUF2509 domain-containing protein YgdB Neighboring gene prepilin-type N-terminal cleavage/methylation domain-containing protein PpdB Neighboring gene prepilin-type N-terminal cleavage/methylation domain-containing protein PpdA Neighboring gene phosphatidylglycerol--prolipoprotein diacylglyceryl transferase Neighboring gene phosphoenolpyruvate-protein phosphotransferase PtsP

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Conservation and Role of Electrostatics in Thymidylate Synthase. Garg D, et al. Sci Rep, 2015 Nov 27. PMID 26612036, Free PMC Article
  2. An analysis of hydrophobic interactions of thymidylate synthase with methotrexate: free energy calculations involving mutant and native structures bound to methotrexate. Reddy RN, et al. J Mol Model, 2010 Feb. PMID 19562390
  3. QM/MM study of thymidylate synthase: enzymatic motions and the temperature dependence of the rate limiting step. Kanaan N, et al. J Phys Chem A, 2009 Mar 12. PMID 19182971, Free PMC Article
  4. Constrained dansyl derivatives reveal bacterial specificity of highly conserved thymidylate synthases. Calò S, et al. Chembiochem, 2008 Mar 25. PMID 18344216
  5. Role of Y94 in proton and hydride transfers catalyzed by thymidylate synthase. Hong B, et al. Biochemistry, 2007 Dec 11. PMID 17999469, Free PMC Article

See all (61) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The measured activities of the E. coli thymidylate synthase (TS) mutants imply that conservation of electrostatics in the region of the active site is important for the activity of TS.
    Title: Conservation and Role of Electrostatics in Thymidylate Synthase.
  2. Data indicate the BGC 945 formed complexes and inhibited E coli and human thymidylate synthase (TS).
    Title: Development and binding mode assessment of N-[4-[2-propyn-1-yl[(6S)-4,6,7,8-tetrahydro-2-(hydroxymethyl)-4-oxo-3H-cyclopenta[g]quinazolin-6-yl]amino]benzoyl]-l-γ-glutamyl-D-glutamic acid (BGC 945), a novel thymidylate synthase inhibitor that targets tumor cells.
  3. findings suggested that Y94 is part of a H-bond network that plays a critical role at a step between the proton and the hydride transfers, presumably the dissociation of H4folate from the covalently bound intermediate.
    Title: Role of Y94 in proton and hydride transfers catalyzed by thymidylate synthase.
  4. N-terminus verified by Edman degradation on complete protein
    Title: Primary structure of the Escherichia coli thyA gene and its thymidylate synthase product.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables RNA binding IEA
Inferred from Electronic Annotation
more info
  
enables magnesium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables thymidylate synthase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables thymidylate synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables thymidylate synthase activity IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity, transferring one-carbon groups IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in dTMP biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within dTMP biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in dTMP biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in dTTP biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in methylation IEA
Inferred from Electronic Annotation
more info
  
involved_in nucleotide biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of translation IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within response to radiation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
thymidylate synthase
NP_417304.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2964361..2965155 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417304.1 thymidylate synthase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417304.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5B9AFL3, A0A8S7S4C6
    Conserved Domains (1) summary
    PRK01827
    Location:1 → 264
    thyA; thymidylate synthase; Reviewed
Nucleotide Protein
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