Cpe carboxypeptidase E [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Cpeprovided by RGD
Official Full Name: carboxypeptidase Eprovided by RGD
Primary source: RGD:2394
See related: Ensembl:ENSRNOG00000043387 AllianceGenome:RGD:2394
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Cph; CARBE
Summary: Enables carboxypeptidase activity; cobalt ion binding activity; and protein domain specific binding activity. Involved in negative regulation of branching morphogenesis of a nerve and peptide metabolic process. Located in several cellular components, including membrane raft; perikaryon; and secretory granule. Human ortholog(s) of this gene implicated in type 2 diabetes mellitus. Orthologous to human CPE (carboxypeptidase E). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Brain (RPKM 2437.3), Heart (RPKM 767.8) and 3 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 16p13

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	16	NC_086034.1 (29796707..29908647)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	16	NC_051351.1 (25030276..25142231)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	16	NC_005115.4 (26906716..27014811)

Chromosome 16 - NC_086034.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Carboxypeptidase E Regulates Activity-Dependent TrkB Neuronal Surface Insertion and Hippocampal Memory.
Title: Carboxypeptidase E Regulates Activity-Dependent TrkB Neuronal Surface Insertion and Hippocampal Memory.
The neuroprotective effect of CPE-DeltaN against neuronal glutamate neurotoxicity was mediated by secreted FGF2.
Title: carboxypeptidase E-ΔN, a neuroprotein transiently expressed during development protects embryonic neurons against glutamate neurotoxicity.
Data indicate that secreted carboxypeptidase E (CPE) promotes PC12 cell survival under metabolic stress.
Title: Carboxypeptidase E promotes cancer cell survival, but inhibits migration and invasion.
These results reveal a novel role for CPE in the regulation of DAT trafficking and DAT-mediated dopamine uptake.
Title: Regulation of dopamine transporter activity by carboxypeptidase E.
CPE mediates the effects of NOS1AP on dendrite morphology.
Title: NOS1AP regulates dendrite patterning of hippocampal neurons through a carboxypeptidase E-mediated pathway.
CPE expression levels were compared among mature retina tissue, RGC-5 cells and its differentiated cells; more CPE and preproNPY protein expression in the tumor cells or tumor tissue were observed
Title: PC2/CPE-mediated pro-protein processing in tumor cells and its differentiated cells or tissues.
Data show that the carboxypeptidase E cytoplasmic tail binds dynactin that recruits kinesins or dynein for driving bi-directional transport of BDNF vesicles to maintain vesicle homeostasis and secretion in hippocampal neurons.
Title: A bi-directional carboxypeptidase E-driven transport mechanism controls BDNF vesicle homeostasis in hippocampal neurons.
our data anticipate the possibility that carboxypeptidase-E, prohormone convertase 1, and prohormone convertase 2 might be potential target for the maturation of somatostatin
Title: Immunohistochemical expression and colocalization of somatostatin, carboxypeptidase-E and prohormone convertases 1 and 2 in rat brain.
Data suggest that ischemia has an adverse impact on the neuropeptide-processing system in the brain and that the lack of an active carboxypeptidase E neuropeptide-processing enzyme exacerbates ischemic brain injury.
Title: Altered biosynthesis of neuropeptide processing enzyme carboxypeptidase E after brain ischemia: molecular mechanism and implication.
Carboxypeptidase E (CPE) is key enzyme for pro-GnRH processing. Reproductive deficits in Cpe(fat/fat) males appear to be due primarily to abnormal sexual behavior.
Title: Deficits in reproduction and pro-gonadotropin-releasing hormone processing in male Cpefat mice.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Cpe (e-PCR), detects polymorphism
- UniSTS:532483

RH94584 (e-PCR)
- UniSTS:119832

Cpe (e-PCR), detects polymorphism
- UniSTS:506813

D16Arb6 (e-PCR), detects polymorphism
- UniSTS:226755

RH94773 (e-PCR)
- UniSTS:120020

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables carboxypeptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables carboxypeptidase activity	ISO Inferred from Sequence Orthology more info
enables cell adhesion molecule binding	IEA Inferred from Electronic Annotation more info
enables cell adhesion molecule binding	ISO Inferred from Sequence Orthology more info
enables cobalt ion binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables metallocarboxypeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables metallocarboxypeptidase activity	IEA Inferred from Electronic Annotation more info
enables metallocarboxypeptidase activity	ISO Inferred from Sequence Orthology more info
enables neurexin family protein binding	IEA Inferred from Electronic Annotation more info
enables neurexin family protein binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in Wnt signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in Wnt signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in cardiac left ventricle morphogenesis	IEA Inferred from Electronic Annotation more info
involved_in cardiac left ventricle morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in enkephalin processing	IDA Inferred from Direct Assay more info	PubMed
involved_in insulin processing	IDA Inferred from Direct Assay more info	PubMed
involved_in insulin processing	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within insulin processing	ISO Inferred from Sequence Orthology more info
involved_in insulin processing	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of branching morphogenesis of a nerve	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in peptide catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in peptide hormone processing	TAS Traceable Author Statement more info	PubMed
involved_in peptide hormone secretion	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within peptide hormone secretion	ISO Inferred from Sequence Orthology more info
involved_in peptide metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in peptide metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in protein localization to membrane	IEA Inferred from Electronic Annotation more info
involved_in protein localization to membrane	ISO Inferred from Sequence Orthology more info
involved_in protein localization to secretory granule	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within protein localization to secretory granule	ISO Inferred from Sequence Orthology more info
involved_in protein processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein processing	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
located_in dendrite	IDA Inferred from Direct Assay more info	PubMed
located_in dense core granule	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular region	IDA Inferred from Direct Assay more info	PubMed
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular space	IDA Inferred from Direct Assay more info	PubMed
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
located_in perikaryon	IDA Inferred from Direct Assay more info	PubMed
located_in secretory granule	IDA Inferred from Direct Assay more info	PubMed
is_active_in secretory granule	ISO Inferred from Sequence Orthology more info
located_in secretory granule membrane	IEA Inferred from Electronic Annotation more info
located_in secretory granule membrane	ISO Inferred from Sequence Orthology more info
located_in secretory granule membrane	TAS Traceable Author Statement more info
located_in synaptic membrane	IDA Inferred from Direct Assay more info	PubMed
located_in transport vesicle membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: carboxypeptidase E

Names: carboxypeptidase H; enkephalin convertase; prohormone-processing carboxypeptidase

NP_037260.2

EC 3.4.17.10

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_013128.2 → NP_037260.2 carboxypeptidase E precursor

Status: PROVISIONAL

Source sequence(s)

JAXUCZ010000016

UniProtKB/Swiss-Prot

P15087

UniProtKB/TrEMBL

D4A8X4, Q5U322

Related

ENSRNOP00000061172.4, ENSRNOT00000064297.4

Conserved Domains (2) summary

cd11308
Location:375 → 449

Peptidase_M14NE-CP-C_like; Peptidase associated domain: C-terminal domain of M14 N/E carboxypeptidase; putative folding, regulation, or interaction domain

cd03865
Location:53 → 371

M14_CPE; Peptidase M14 carboxypeptidase subfamily N/E-like; Carboxypeptidase E subgroup