MED30 mediator complex subunit 30 [Homo sapiens (human)] - Gene

Summary

Official Symbol: MED30provided by HGNC
Official Full Name: mediator complex subunit 30provided by HGNC
Primary source: HGNC:HGNC:23032
See related: Ensembl:ENSG00000164758 MIM:610237; AllianceGenome:HGNC:23032
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: MED30S; THRAP6; TRAP25
Summary: The multiprotein TRAP/Mediator complex facilitates gene expression through a wide variety of transcriptional activators. MED30 is a component of this complex that appears to be metazoan specific (Baek et al., 2002 [PubMed 11909976]).[supplied by OMIM, Nov 2010]
Expression: Ubiquitous expression in bone marrow (RPKM 9.2), lymph node (RPKM 5.0) and 24 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 8q24.11

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
RS_2023_10	current	GRCh38.p14 (GCF_000001405.40)	8	NC_000008.11 (117520713..117540262)
RS_2023_10	current	T2T-CHM13v2.0 (GCF_009914755.1)	8	NC_060932.1 (118648865..118668421)
105.20220307	previous assembly	GRCh37.p13 (GCF_000001405.25)	8	NC_000008.10 (118532952..118552501)

Chromosome 8 - NC_000008.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HIF1alpha and p53 Regulated MED30, a Mediator Complex Subunit, is Involved in Regulation of Glioblastoma Pathogenesis and Temozolomide Resistance.
Title: HIF1α and p53 Regulated MED30, a Mediator Complex Subunit, is Involved in Regulation of Glioblastoma Pathogenesis and Temozolomide Resistance.
MED30 seems to be involved in the progression of the renal cell carcinoma (RCC).
Title: The knockdown of the mediator complex subunit MED30 suppresses the proliferation and migration of renal cell carcinoma cells.
Knockdown of MED30 led to reduction of the tumour parameters proliferation, migration, and invasion in the bladder cancer (BCa) cell lines.
Title: The Contrasting Role of the Mediator Subunit MED30 in the Progression of Bladder Cancer.
analysis of a novel transcript of MED30, termed MED30 short (MED30s) generated by alternative splicing
Title: Mediator subunits: gene expression pattern, a novel transcript identification and nuclear localization in human endothelial progenitor cells.
Requirement of TRAP/mediator for both activator-independent and activator-dependent transcription in conjunction with TFIID-associated TAF(II)s.
Title: Requirement of TRAP/mediator for both activator-independent and activator-dependent transcription in conjunction with TFIID-associated TAF(II)s.

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Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Two new susceptibility loci for Kawasaki disease identified through genome-wide association analysis. EBI GWAS Catalog EBI GWAS CatalogPubMed

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Replication interactions

Interaction	Pubs
Knockdown of mediator complex subunit 30 (MED30) by siRNA inhibits HIV-1 replication in HeLa-derived TZM-bl cells	PubMed

Protein interactions

Protein	Gene	Interaction	Pubs
Tat	tat	SiRNA knockdown of d MED30 shows a modest inhibition in HIV-1 Tat-mediated transcription levels as compared to control	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables nuclear receptor coactivator activity	NAS Non-traceable Author Statement more info	PubMed
enables nuclear thyroid hormone receptor binding	IDA Inferred from Direct Assay more info	PubMed
enables nuclear vitamin D receptor binding	NAS Non-traceable Author Statement more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transcription coactivator activity	IDA Inferred from Direct Assay more info	PubMed
enables transcription coregulator activity	IBA Inferred from Biological aspect of Ancestor more info
enables transcription coregulator activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin protein ligase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in RNA polymerase II preinitiation complex assembly	NAS Non-traceable Author Statement more info	PubMed
involved_in positive regulation of DNA-templated transcription	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription elongation by RNA polymerase II	NAS Non-traceable Author Statement more info	PubMed
involved_in positive regulation of transcription initiation by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription initiation by RNA polymerase II	NAS Non-traceable Author Statement more info	PubMed
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in somatic stem cell population maintenance	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of core mediator complex	IPI Inferred from Physical Interaction more info	PubMed
part_of mediator complex	IBA Inferred from Biological aspect of Ancestor more info
part_of mediator complex	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	TAS Traceable Author Statement more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
part_of ubiquitin ligase complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: mediator of RNA polymerase II transcription subunit 30

Names: TRAP/Mediator complex component TRAP25; putative mediator of RNA polymerase II transcription subunit 30; thyroid hormone receptor-associated protein 6; thyroid hormone receptor-associated protein complex 25 kDa component

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001282986.2 → NP_001269915.1 mediator of RNA polymerase II transcription subunit 30 isoform 2

See identical proteins and their annotated locations for NP_001269915.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region compared to variant 1. The encoded isoform (2) is shorter than isoofrm 1.

Source sequence(s)

AA812233, AC024329, AC087361, FM179284

Consensus CDS

CCDS64959.1

UniProtKB/Swiss-Prot

Q96HR3

Related

ENSP00000431051.1, ENST00000522839.1

Conserved Domains (1) summary

pfam11315
Location:29 → 141

Med30; Mediator complex subunit 30
NM_001363182.2 → NP_001350111.1 mediator of RNA polymerase II transcription subunit 30 isoform 3

Status: VALIDATED

Source sequence(s)

AC024329, AC087361, AY083305

Conserved Domains (1) summary

pfam11315
Location:29 → 147

Med30; Mediator complex subunit 30
NM_080651.4 → NP_542382.1 mediator of RNA polymerase II transcription subunit 30 isoform 1

See identical proteins and their annotated locations for NP_542382.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest isoform (1).

Source sequence(s)

AA812233, AC024329, AC087361, AY083305

Consensus CDS

CCDS6323.1

UniProtKB/Swiss-Prot

C6GKU9, Q96HR3

Related

ENSP00000297347.3, ENST00000297347.7

Conserved Domains (1) summary

pfam11315
Location:29 → 176

Med30; Mediator complex subunit 30