Ldhb lactate dehydrogenase B [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ldhbprovided by MGI
Official Full Name: lactate dehydrogenase Bprovided by MGI
Primary source: MGI:MGI:96763
See related: Ensembl:ENSMUSG00000030246 AllianceGenome:MGI:96763
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Ldh2; H-Ldh; LDH-B; LDH-H; Ldh-2
Summary: This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have also been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes 1 and 19. [provided by RefSeq, Feb 2016]
Expression: Broad expression in kidney adult (RPKM 644.8), heart adult (RPKM 618.4) and 18 other tissues See more
Orthologs: human all
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Genomic context

Location:: 6 G2; 6 74.17 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	6	NC_000072.7 (142435975..142453683, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	6	NC_000072.6 (142490249..142507957, complement)

Chromosome 6 - NC_000072.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Lactate dehydrogenase B regulates macrophage metabolism in the tumor microenvironment.
Title: Lactate dehydrogenase B regulates macrophage metabolism in the tumor microenvironment.
Expression of lactate dehydrogenase A and B isoforms in the mouse kidney.
Title: Expression of lactate dehydrogenase A and B isoforms in the mouse kidney.
these results identified a previously unrecognized Ldhb-driven alteration in muscle mitochondrial function and suggested a mechanism for the adaptive metabolic response induced by exercise training.
Title: Exercise Inducible Lactate Dehydrogenase B Regulates Mitochondrial Function in Skeletal Muscle.
Findings suggest that RANKL protein-induced lactate dehydrogenase (LDH) activation stimulates glycolytic and mitochondrial respiratory metabolism via transcription factor NFATc1 signaling.
Title: Accelerated Lactate Dehydrogenase Activity Potentiates Osteoclastogenesis via NFATc1 Signaling.
drs regulates glucose metabolism via LDH-B. Downregulating drs may contribute to the Warburg effect, which is closely associated with malignant progression of cancer cells.
Title: The drs tumor suppressor regulates glucose metabolism via lactate dehydrogenase-B.
Exercise-induced changes in tumour LDH-B and MCT1 expression are modulated by oestrogen-related receptor alpha in breast cancer-bearing BALB/c mice
Title: Exercise-induced changes in tumour LDH-B and MCT1 expression are modulated by oestrogen-related receptor alpha in breast cancer-bearing BALB/c mice.
Data show that PI3K/Akt signalling pathway and lactate dehydrogenase (LDH) play a crucial role in increase of cardiac ATP-binding cassette transporter sub-family C member 9 (SUR2A) induced by in vivo exposure to 18% oxygen.
Title: Mild hypoxia in vivo regulates cardioprotective SUR2A: A role for Akt and LDH.
PGC-1alpha in skeletal muscle drives the expression of lactate dehydrogenase (LDH) B in an estrogen-related receptor-alpha-dependent manner.
Title: Skeletal muscle PGC-1α controls whole-body lactate homeostasis through estrogen-related receptor α-dependent activation of LDH B and repression of LDH A.
the Ldhb gene, which encodes the H isoform of lactate dehydrogenase, as a robust PPARgamma target in trophoblasts.
Title: Placental PPARγ regulates spatiotemporally diverse genes and a unique metabolic network.
findings offer proof of concept for targeting LDHB as a therapeutic strategy in cancers driven by aberrant activation of the RTK-PI3K-AKT-mTOR signaling cascade
Title: Lactate dehydrogenase B is critical for hyperactive mTOR-mediated tumorigenesis.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (3)
Endonuclease-mediated (5)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AI790582 (e-PCR)
- UniSTS:179489

Ldhb (e-PCR), detects polymorphism
- UniSTS:527402

NoName (e-PCR)
- UniSTS:224332

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables L-lactate dehydrogenase activity	IBA Inferred from Biological aspect of Ancestor more info
enables L-lactate dehydrogenase activity	IDA Inferred from Direct Assay more info	PubMed
enables L-lactate dehydrogenase activity	ISO Inferred from Sequence Orthology more info
enables NAD binding	ISO Inferred from Sequence Orthology more info
enables catalytic activity	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables kinase binding	ISO Inferred from Sequence Orthology more info
enables lactate dehydrogenase activity	ISO Inferred from Sequence Orthology more info
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in NAD metabolic process	ISO Inferred from Sequence Orthology more info
involved_in carboxylic acid metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within lactate biosynthetic process from pyruvate	IC Inferred by Curator more info
involved_in lactate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in lactate metabolic process	ISO Inferred from Sequence Orthology more info
involved_in pyruvate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in pyruvate metabolic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in membrane raft	ISO Inferred from Sequence Orthology more info
located_in mitochondrial inner membrane	ISO Inferred from Sequence Orthology more info
located_in mitochondrion	HDA more info	PubMed
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in myelin sheath	HDA more info	PubMed
part_of oxidoreductase complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: L-lactate dehydrogenase B chain

Names: LDH heart subunit; lactate dehydrogenase 2, B chain

NP_001289694.1

EC 1.1.1.27

NP_001303251.1

EC 1.1.1.27

NP_032518.1

EC 1.1.1.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001302765.1 → NP_001289694.1 L-lactate dehydrogenase B chain isoform 2

Status: REVIEWED

Description

Transcript Variant: This variant (2) lacks an exon in the 3' coding region, which results in a frameshift and a different 3' UTR compared to variant 1. The encoded isoform (2) is shorter with a distinct C-terminus compared to isoform Ldhb.

Source sequence(s)

AK019391, BC046755, CX208147

UniProtKB/Swiss-Prot

P16125

Conserved Domains (1) summary

cd05293
Location:20 → 246

LDH_1; A subgroup of L-lactate dehydrogenases
NM_001316322.1 → NP_001303251.1 L-lactate dehydrogenase B chain isoform Ldhbx

Status: REVIEWED

Description

Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (Ldhb) results from translation termination at the upstream UGA stop codon, while the longer isoform (Ldhbx) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the longer, C-terminally extended isoform (Ldhbx), which is localized in the peroxisomes. As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), its location in the longer isoform is denoted by an 'X'.

Source sequence(s)

AK019391, BC046755

UniProtKB/TrEMBL

A0A6I8MX27

Related

ENSMUSP00000159378.2, ENSMUST00000239395.2

Conserved Domains (2) summary

cd05293
Location:20 → 330

LDH_1; A subgroup of L-lactate dehydrogenases

TIGR01771
Location:26 → 326

L-LDH-NAD; L-lactate dehydrogenase
NM_008492.3 → NP_032518.1 L-lactate dehydrogenase B chain isoform Ldhb

See identical proteins and their annotated locations for NP_032518.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (Ldhb) results from translation termination at the upstream UGA stop codon, while the longer isoform (Ldhbx) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the shorter isoform (Ldhb), which is localized in the cytosol.

Source sequence(s)

AK019391, BC046755

Consensus CDS

CCDS20684.1

UniProtKB/Swiss-Prot

P16125, Q545Y4

Related

ENSMUSP00000032373.6, ENSMUST00000032373.12

Conserved Domains (2) summary

cd05293
Location:20 → 330

LDH_1; A subgroup of L-lactate dehydrogenases

TIGR01771
Location:26 → 326

L-LDH-NAD; L-lactate dehydrogenase