Flot1 flotillin 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Flot1provided by RGD
Official Full Name: flotillin 1provided by RGD
Primary source: RGD:70991
See related: Ensembl:ENSRNOG00000000826 AllianceGenome:RGD:70991
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Rareg
Summary: Enables ionotropic glutamate receptor binding activity and protease binding activity. Predicted to be involved in several processes, including positive regulation of cell-cell adhesion; positive regulation of cellular component organization; and protein localization to membrane. Predicted to act upstream of or within axonogenesis and regulation of Rho protein signal transduction. Located in caveola. Part of flotillin complex. Is active in GABA-ergic synapse; glutamatergic synapse; and presynaptic active zone. Orthologous to human FLOT1 (flotillin 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Heart (RPKM 171.0), Adrenal (RPKM 153.7) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 20p12

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	20	NC_086038.1 (2922662..2932906, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	20	NC_051355.1 (2917864..2927993, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	20	NC_005119.4 (3427890..3438418, complement)

Chromosome 20 - NC_086038.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

the presence of Flotillin-1, in addition to Flotillin-2 in the cardiac intercalated disc, is reported.
Title: Flotillins in the intercalated disc are potential modulators of cardiac excitability.
Data suggest that flotillin-1 and flotillin-2 function as thyroglobulin-binding proteins in lipid rafts of thyrocytes; these interactions appear to exert negative-feedback effects on gene expression in thyrocytes.
Title: A Novel Role for Flotillin-Containing Lipid Rafts in Negative-Feedback Regulation of Thyroid-Specific Gene Expression by Thyroglobulin.
Study illustrates early developmental changes in the cellular and subcellular localization of flotillin-1 in the rat visual cortex and suggests that flotillin-1 was transported mainly into presynaptic terminals
Title: Developmental changes in the flotillin-1 expression pattern of the rat visual cortex.
Reggie-1 nas reggie-2 are localized in microdomains different from caveolae, and the function of reggies is different from and independent of caveolin-1.
Title: Reggie-1 and reggie-2 localize in non-caveolar rafts in epithelial cells: cellular localization is not dependent on the expression of caveolin proteins.
Flot-1 enhances the formation of glutamatergic synapses but not GABAergic synapses in cultured neurons from the hippocampus.
Title: Flotillin-1 promotes formation of glutamatergic synapses in hippocampal neurons.
flotillins have a role in NPC1L1-mediated cholesterol uptake and NPC1L1-flotillins-postive cholesterol-enriched membrane microdomains are involved in the mechanism for efficient cholesterol absorption
Title: Flotillins play an essential role in Niemann-Pick C1-like 1-mediated cholesterol uptake.
Data reveal a flotillin-1 signaling pathway for hippocampal neurite branching that is regulated by synaptic adhesion-like molecule 4.
Title: Flotillin-1 mediates neurite branching induced by synaptic adhesion-like molecule 4 in hippocampal neurons.
NMDARs were significantly more colocalized with flot-1 than flot-2 in cultured hippocampal neurons
Title: NMDA receptors interact with flotillin-1 and -2, lipid raft-associated proteins.
Data suggest that flotillin-1 participates in the developmental process of Sertoli cells and is involved in the regulation of spermatogenesis.
Title: Immunohistochemical study of flotillin-1 in the rat testis during postnatal development.
Flotillin-1 is an essential molecule in IgE receptor-mediated mast cell activation, and regulates the kinase activity of Lyn in lipid rafts.
Title: Flotillin-1 regulates IgE receptor-mediated signaling in rat basophilic leukemia (RBL-2H3) cells.

Submit: New GeneRIF Correction See all GeneRIFs (16)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

MARC_5855-5856:997299371:1 (e-PCR)
- UniSTS:269590

D20Yum36 (e-PCR)
- UniSTS:526111

UniSTS:496739 (e-PCR)
- UniSTS:496739

Ier3 (e-PCR), detects polymorphism
- UniSTS:496739

RH131920 (e-PCR)
- UniSTS:215203

RH129082 (e-PCR)
- UniSTS:212390

RH140901 (e-PCR)
- UniSTS:222917

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ionotropic glutamate receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables protease binding	IBA Inferred from Biological aspect of Ancestor more info
enables protease binding	IPI Inferred from Physical Interaction more info	PubMed
enables protease binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within axonogenesis	ISO Inferred from Sequence Orthology more info
involved_in cellular response to exogenous dsRNA	ISO Inferred from Sequence Orthology more info
involved_in dsRNA transport	ISO Inferred from Sequence Orthology more info
involved_in extracellular matrix disassembly	ISO Inferred from Sequence Orthology more info
involved_in plasma membrane raft assembly	ISO Inferred from Sequence Orthology more info
acts_upstream_of_positive_effect plasma membrane raft organization	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of canonical NF-kappaB signal transduction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell junction assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of cell junction assembly	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell-cell adhesion mediated by cadherin	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of cell-cell adhesion mediated by cadherin	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cytokine production	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of endocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of endocytosis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of heterotypic cell-cell adhesion	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of interferon-beta production	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of myoblast fusion	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of skeletal muscle tissue development	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of synaptic transmission, dopaminergic	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of toll-like receptor 3 signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in protein kinase C signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein kinase C signaling	ISO Inferred from Sequence Orthology more info
involved_in protein localization to plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within protein localization to plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in protein localization to plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in protein stabilization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of Rho protein signal transduction	ISO Inferred from Sequence Orthology more info
involved_in regulation of neurotransmitter uptake	ISO Inferred from Sequence Orthology more info
involved_in regulation of receptor internalization	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of receptor internalization	ISO Inferred from Sequence Orthology more info
NOT involved_in regulation of toll-like receptor 4 signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in response to endoplasmic reticulum stress	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of COP9 signalosome	ISO Inferred from Sequence Orthology more info
is_active_in GABA-ergic synapse	EXP Inferred from Experiment more info	PubMed
is_active_in GABA-ergic synapse	IDA Inferred from Direct Assay more info	PubMed
located_in adherens junction	ISO Inferred from Sequence Orthology more info
located_in apical part of cell	ISO Inferred from Sequence Orthology more info
NOT located_in apical plasma membrane	ISO Inferred from Sequence Orthology more info
located_in basolateral plasma membrane	ISO Inferred from Sequence Orthology more info
located_in caveola	IDA Inferred from Direct Assay more info	PubMed
located_in caveola	ISO Inferred from Sequence Orthology more info
located_in cell-cell contact zone	ISO Inferred from Sequence Orthology more info
located_in cell-cell junction	ISO Inferred from Sequence Orthology more info
located_in centriolar satellite	ISO Inferred from Sequence Orthology more info
located_in cortical actin cytoskeleton	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasmic vesicle	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasmic vesicle	ISO Inferred from Sequence Orthology more info
is_active_in dopaminergic synapse	ISO Inferred from Sequence Orthology more info
located_in early endosome	ISO Inferred from Sequence Orthology more info
located_in endosome	IEA Inferred from Electronic Annotation more info
located_in endosome	ISO Inferred from Sequence Orthology more info
located_in endosome	ISS Inferred from Sequence or Structural Similarity more info
located_in external side of plasma membrane	ISO Inferred from Sequence Orthology more info
part_of flotillin complex	IBA Inferred from Biological aspect of Ancestor more info
part_of flotillin complex	IDA Inferred from Direct Assay more info	PubMed
part_of flotillin complex	IEA Inferred from Electronic Annotation more info
part_of flotillin complex	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	EXP Inferred from Experiment more info	PubMed
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
located_in lamellipodium	ISO Inferred from Sequence Orthology more info
located_in melanosome	IEA Inferred from Electronic Annotation more info
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in membrane raft	ISO Inferred from Sequence Orthology more info
located_in microtubule organizing center	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane raft	ISO Inferred from Sequence Orthology more info
is_active_in presynapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in presynaptic active zone	EXP Inferred from Experiment more info	PubMed
is_active_in presynaptic active zone	IDA Inferred from Direct Assay more info	PubMed
located_in sarcolemma	ISO Inferred from Sequence Orthology more info
is_active_in synapse	IDA Inferred from Direct Assay more info	PubMed
located_in uropod	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: flotillin-1

Names: REG-2; reggie-2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_022701.2 → NP_073192.2 flotillin-1

See identical proteins and their annotated locations for NP_073192.2

Status: VALIDATED

Source sequence(s)

CB324456, CB326388, CK359020, DY569467

UniProtKB/Swiss-Prot

Q9Z1E1, Q9Z1E2

UniProtKB/TrEMBL

A0A8L2PZR2, A6KT86

Conserved Domains (2) summary

COG2268
Location:4 → 424

YqiK; Uncharacterized membrane protein YqiK, contains Band7/PHB/SPFH domain [Function unknown]

cd03399
Location:30 → 175

SPFH_flotillin; Flotillin or reggie family; SPFH (stomatin, prohibitin, flotillin, and HflK/C) superfamily

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086038.1 Reference GRCr8

Range

2922662..2932906 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006256024.5 → XP_006256086.1 flotillin-1 isoform X1

See identical proteins and their annotated locations for XP_006256086.1

UniProtKB/Swiss-Prot

Q9Z1E1, Q9Z1E2

UniProtKB/TrEMBL

A0A8L2PZR2, A6KT86

Related

ENSRNOP00000071275.1

Conserved Domains (2) summary

COG2268
Location:4 → 424

YqiK; Uncharacterized membrane protein YqiK, contains Band7/PHB/SPFH domain [Function unknown]

cd03399
Location:30 → 175

SPFH_flotillin; Flotillin or reggie family; SPFH (stomatin, prohibitin, flotillin, and HflK/C) superfamily
XM_063279497.1 → XP_063135567.1 flotillin-1 isoform X2
XM_063279499.1 → XP_063135569.1 flotillin-1 isoform X3