Alox12 arachidonate 12-lipoxygenase [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Alox12provided by MGI
Official Full Name: arachidonate 12-lipoxygenaseprovided by MGI
Primary source: MGI:MGI:87998
See related: Ensembl:ENSMUSG00000000320 AllianceGenome:MGI:87998
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: P-12LO; Alox12p; 9930022G08Rik
Summary: Enables arachidonate 12(S)-lipoxygenase activity and linoleate 13S-lipoxygenase activity. Involved in establishment of skin barrier; fatty acid metabolic process; and negative regulation of platelet aggregation. Located in cytoplasm. Is expressed in alimentary system; liver; liver left lobe; and liver right lobe. Orthologous to human ALOX12 (arachidonate 12-lipoxygenase, 12S type). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in genital fat pad adult (RPKM 9.9), spleen adult (RPKM 9.6) and 19 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 B3; 11 42.99 cM

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (70132283..70146206, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (70241457..70255380, complement)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Neonatal imprinting of alveolar macrophages via neutrophil-derived 12-HETE.
Title: Neonatal imprinting of alveolar macrophages via neutrophil-derived 12-HETE.
Key Role of 12-Lipoxygenase and Its Metabolite 12-Hydroxyeicosatetraenoic Acid (12-HETE) in Diabetic Retinopathy.
Title: Key Role of 12-Lipoxygenase and Its Metabolite 12-Hydroxyeicosatetraenoic Acid (12-HETE) in Diabetic Retinopathy.
Hydrogen sulfide guards myoblasts from ferroptosis by inhibiting ALOX12 acetylation.
Title: Hydrogen sulfide guards myoblasts from ferroptosis by inhibiting ALOX12 acetylation.
Alox12/15 Deficiency Exacerbates, While Lipoxin A4 Ameliorates Hepatic Inflammation in Murine Alcoholic Hepatitis.
Title: Alox12/15 Deficiency Exacerbates, While Lipoxin A(4) Ameliorates Hepatic Inflammation in Murine Alcoholic Hepatitis.
Arachidonate 12S-lipoxygenase of platelet-type in hepatic stellate cells of methionine and choline-deficient diet-fed mice.
Title: Arachidonate 12S-lipoxygenase of platelet-type in hepatic stellate cells of methionine and choline-deficient diet-fed mice.
Knockout of ALOX12 protects against spinal cord injury-mediated nerve injury by inhibition of inflammation and apoptosis.
Title: Knockout of ALOX12 protects against spinal cord injury-mediated nerve injury by inhibition of inflammation and apoptosis.
these results indicate that Alox12 loss activates a compensatory increase in Alox15 that sensitizes mouse beta cells to oxidative stress.
Title: Platelet-type 12-lipoxygenase deletion provokes a compensatory 12/15-lipoxygenase increase that exacerbates oxidative stress in mouse islet β cells.
12(S)-HETrE, a 12-lipoxygenase oxylipin of dihomo-gamma-linolenic acid, inhibits thrombosis via Galphas signaling in platelets.
Title: 12(S)-HETrE, a 12-Lipoxygenase Oxylipin of Dihomo-γ-Linolenic Acid, Inhibits Thrombosis via Gαs Signaling in Platelets.
the amount of LPL expressed in muscle and heart governed both the binding of chylomicron particles and the assimilation of chylomicron lipids in the tissue.
Title: A lipidomic screen of hyperglycemia-treated HRECs links 12/15-Lipoxygenase to microvascular dysfunction during diabetic retinopathy via NADPH oxidase.
Data show that the coordinated action of phospholipase A2 IIA (sPLA2-IIA) and 12-lipoxygenase (12-LO) promotes inflammatory arthritis.
Title: Platelet microparticles are internalized in neutrophils via the concerted activity of 12-lipoxygenase and secreted phospholipase A2-IIA.

Submit: New GeneRIF Correction See all GeneRIFs (49)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (1) 1 citation
Endonuclease-mediated (4) 1 citation

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables arachidonate 12(S)-lipoxygenase activity	IBA Inferred from Biological aspect of Ancestor more info
enables arachidonate 12(S)-lipoxygenase activity	IDA Inferred from Direct Assay more info	PubMed
enables arachidonate 12(S)-lipoxygenase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables arachidonate 12(S)-lipoxygenase activity	ISO Inferred from Sequence Orthology more info
enables arachidonate 15-lipoxygenase activity	IBA Inferred from Biological aspect of Ancestor more info
enables arachidonate 15-lipoxygenase activity	ISO Inferred from Sequence Orthology more info
enables dioxygenase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables iron ion binding	IEA Inferred from Electronic Annotation more info
enables linoleate 13S-lipoxygenase activity	IBA Inferred from Biological aspect of Ancestor more info
enables linoleate 13S-lipoxygenase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables linoleate 13S-lipoxygenase activity	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in arachidonic acid metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in arachidonic acid metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in arachidonic acid metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in arachidonic acid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in establishment of skin barrier	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within fatty acid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in fatty acid oxidation	IDA Inferred from Direct Assay more info	PubMed
involved_in fatty acid oxidation	ISO Inferred from Sequence Orthology more info
involved_in hepoxilin biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in lipid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in lipid oxidation	IBA Inferred from Biological aspect of Ancestor more info
involved_in lipoxin A4 biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in lipoxin B4 biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in lipoxygenase pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in lipoxygenase pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in lipoxygenase pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lipoxygenase pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of muscle cell apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of platelet aggregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of blood vessel endothelial cell migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of endothelial tube morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of mitochondrial depolarization	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of reactive oxygen species biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of smooth muscle cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in unsaturated fatty acid metabolic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in membrane	ISO Inferred from Sequence Orthology more info
is_active_in sarcolemma	IBA Inferred from Biological aspect of Ancestor more info
located_in sarcolemma	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: polyunsaturated fatty acid lipoxygenase ALOX12

Names: 12S-LOX; 12S-lipoxygenase; arachidonate (12S)-lipoxygenase; arachidonate (15S)-lipoxygenase; arachidonate 12-lipoxygenase, 12S-type; arachidonate 15-lipoxygenase,15S-type; linoleate 13S-lipoxygenase; lipoxin synthase 12-LO; platelet-type lipoxygenase 12

NP_001318047.1

EC 1.13.11.31

EC 1.13.11.33

NP_031466.2

EC 1.13.11.31

EC 1.13.11.33

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001331118.1 → NP_001318047.1 polyunsaturated fatty acid lipoxygenase ALOX12 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR and uses a downstream translation start site compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1.

Source sequence(s)

AK036960, AK087724, AL669869

UniProtKB/Swiss-Prot

P39655

Conserved Domains (1) summary

pfam00305
Location:58 → 491

Lipoxygenase; Lipoxygenase
NM_007440.5 → NP_031466.2 polyunsaturated fatty acid lipoxygenase ALOX12 isoform 1

See identical proteins and their annotated locations for NP_031466.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longer isoform (1).

Source sequence(s)

AK087724, AL669869

Consensus CDS

CCDS24942.1

UniProtKB/Swiss-Prot

P39655, Q8BHG4

UniProtKB/TrEMBL

A2CF85, Q3T9I9, Q3T9Y3

Related

ENSMUSP00000000329.3, ENSMUST00000000329.9

Conserved Domains (2) summary

cd01753
Location:2 → 111

PLAT_LOX; PLAT domain of 12/15-lipoxygenase. As a unique subfamily of the mammalian lipoxygenases, they catalyze enzymatic lipid peroxidation in complex biological structures via direct dioxygenation of phospholipids and cholesterol esters of biomembranes and ...

pfam00305
Location:221 → 654

Lipoxygenase