Gzmf granzyme F [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Gzmfprovided by MGI
Official Full Name: granzyme Fprovided by MGI
Primary source: MGI:MGI:109254
See related: Ensembl:ENSMUSG00000015441 AllianceGenome:MGI:109254
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: CCP4; Ctla7; Ctla-7; MCSP-3
Summary: Predicted to enable serine-type endopeptidase activity. Predicted to be involved in several processes, including granzyme-mediated programmed cell death signaling pathway; natural killer cell mediated cytotoxicity; and pyroptosis. Predicted to act upstream of or within cytolysis. Predicted to be located in cytolytic granule. Predicted to be active in cytoplasm and intracellular membrane-bounded organelle. Is expressed in extraembryonic component; placenta; spongiotrophoblast; and trophoblast giant cell. Orthologous to human GZMB (granzyme B). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Restricted expression toward placenta adult (RPKM 30.6) See more
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Genomic context

Location:: 14 C3; 14 28.19 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	14	NC_000080.7 (56442720..56448864, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	14	NC_000080.6 (56205263..56211407, complement)

Chromosome 14 - NC_000080.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Granzyme F: Exhaustion Marker and Modulator of Chimeric Antigen Receptor T Cell-Mediated Cytotoxicity.
Title: Granzyme F: Exhaustion Marker and Modulator of Chimeric Antigen Receptor T Cell-Mediated Cytotoxicity.
Nna1 and CCP4 remove the C-terminal glutamate from substrates with two or more glutamates, whereas CCP6 requires four or more glutamates. CCP4 behaves as a promiscuous glutamase with little preference for chain length or neighboring amino acid composition
Title: Comparison of the enzymatic and functional properties of three cytosolic carboxypeptidase family members.
showed that GzmF impairs mitochondrial electron transport to abolish ATP generation; ATP decline may contribute to a failure of apoptosome formation, leading to caspase inactivation.
Title: Granzyme F induces a novel death pathway characterized by Bid-independent cytochrome c release without caspase activation.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Gzmf (e-PCR), detects polymorphism
- UniSTS:532236

D14Mit84 (e-PCR), detects polymorphism
- UniSTS:141988

NoName (e-PCR)
- UniSTS:492965

Gzmf (e-PCR), detects polymorphism
- UniSTS:143285

D971 (e-PCR)
- UniSTS:127440

D14Mit84.2 (e-PCR), detects polymorphism
- UniSTS:127441

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables peptidase activity	IEA Inferred from Electronic Annotation more info
enables serine-type endopeptidase activity	ISO Inferred from Sequence Orthology more info
enables serine-type peptidase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within killing of cells of another organism	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within proteolysis	IEA Inferred from Electronic Annotation more info
involved_in proteolysis	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytolytic granule	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in intracellular membrane-bounded organelle	IBA Inferred from Biological aspect of Ancestor more info
located_in lysosome	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: granzyme F

Names: AKA granzyme G; C134; CTL serine protease 3; MCSP3; cytotoxic cell protease 4; cytotoxic serine protease 3; granzyme G

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_010374.3 → NP_034504.1 granzyme F precursor

See identical proteins and their annotated locations for NP_034504.1

Status: PROVISIONAL

Source sequence(s)

BC100311

Consensus CDS

CCDS36936.1

UniProtKB/Swiss-Prot

P08883

UniProtKB/TrEMBL

Q497Z7

Related

ENSMUSP00000022757.4, ENSMUST00000022757.5

Conserved Domains (2) summary

smart00020
Location:20 → 241

Tryp_SPc; Trypsin-like serine protease

cd00190
Location:21 → 244

Tryp_SPc; Trypsin-like serine protease; Many of these are synthesized as inactive precursor zymogens that are cleaved during limited proteolysis to generate their active forms. Alignment contains also inactive enzymes that have substitutions of the catalytic triad ...