Mical2 microtubule associated monooxygenase, calponin and LIM domain containing 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Mical2provided by MGI
Official Full Name: microtubule associated monooxygenase, calponin and LIM domain containing 2provided by MGI
Primary source: MGI:MGI:2444947
See related: Ensembl:ENSMUSG00000038244 AllianceGenome:MGI:2444947
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: MICAL-2; Micalcl; mKIAA0750; 9530064J02; 4921517J23Rik; 5330438E18Rik
Summary: Enables several functions, including NAD(P)H oxidase H2O2-forming activity; actin binding activity; and mitogen-activated protein kinase binding activity. Involved in actin filament depolymerization and sulfur oxidation. Located in cytoplasm. Is expressed in lower jaw molar and upper jaw molar. Orthologous to human MICAL2 (microtubule associated monooxygenase, calponin and LIM domain containing 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in cortex adult (RPKM 29.1), lung adult (RPKM 19.7) and 20 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7 F1; 7 58.75 cM

Exon count:: 39

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (111825032..112012311)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (112225825..112355194)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

EBITEIN1 is a phosphoprotein and a downstream interactor of ERK2 that participates in the intracellular signal transduction pathway mediating the morphogenetic development of round spermatids into spermatozoa.
Title: ERK2-binding domain is required for phosphorylation of EBITEIN1, a potential downstream interactor of ERK2.
Based on empirical and bioinformatic analyses, we propose two domains in EBITEIN1. One is the minimal sequence for binding ERK2 (EB domain) and the other is the EBITEIN1 C-terminal domain (ECT domain).
Title: Identification of ERK2-binding domain of EBITEIN1, a novel ERK2-binding protein.
MICAL2 is essential for myogenic lineage commitment.
Title: MICAL2 is essential for myogenic lineage commitment.
MICAL2 regulates myofibroblasts differentiation in epidural fibrosis via SRF/MRTF-A signaling pathway.
Title: MICAL2 regulates myofibroblasts differentiation in epidural fibrosis via SRF/MRTF-A signaling pathway.
In pulmonary artery hypertension miR-205-5p suppressed pulmonary artery smooth muscle cell proliferation by targeting MICAL2, which activated ERK1/2 signaling.
Title: miR-205-5p suppresses pulmonary vascular smooth muscle cell proliferation by targeting MICAL2-mediated Erk1/2 signaling.
show that this strategy is used by MICAL-2. Thus, our data suggest that MICAL-2 could regulate catalysis through the monooxygenase domain alone
Title: Actin stimulates reduction of the MICAL-2 monooxygenase domain.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables FAD binding	IEA Inferred from Electronic Annotation more info
enables NAD(P)H oxidase H2O2-forming activity	IDA Inferred from Direct Assay more info	PubMed
enables actin binding	IDA Inferred from Direct Assay more info	PubMed
enables actin binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables mitogen-activated protein kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables monooxygenase activity	ISO Inferred from Sequence Orthology more info
enables oxidoreductase activity	ISO Inferred from Sequence Orthology more info
enables oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in actin filament depolymerization	IDA Inferred from Direct Assay more info	PubMed
involved_in actin filament depolymerization	ISO Inferred from Sequence Orthology more info
involved_in cytoskeleton organization	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in sulfur oxidation	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
is_active_in actin filament	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: F-actin-monooxygenase MICAL2; [F-actin]-monooxygenase MICAL2

Names: ERK2-binding testicular protein 1; MICAL C-terminal like; MICAL C-terminal-like protein; MICAL C-terminal-like protein-like; [F-actin]-methionine sulfoxide oxidase MICAL2; ebitein-1; ebitein1; mMical2; mical-cL; microtubule associated monoxygenase, calponin and LIM domain containing 2; molecule interacting with CasL protein 2; protein MICAL-2; protein-methionine sulfoxide oxidase MICAL2

NP_001180234.1

EC 1.14.13.225

NP_001368791.1

EC 1.14.13.225

NP_001382793.1

EC 1.14.13.225

NP_796256.1

EC 1.14.13.225

XP_006508017.1

EC 1.14.13.225

XP_030098545.1

EC 1.14.13.225

XP_036009064.1

EC 1.14.13.225

XP_036009065.1

EC 1.14.13.225

XP_036009066.1

EC 1.14.13.225

XP_036009067.1

EC 1.14.13.225

XP_036009068.1

EC 1.14.13.225

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001193305.1 → NP_001180234.1 [F-actin]-monooxygenase MICAL2 isoform A

See identical proteins and their annotated locations for NP_001180234.1

Status: VALIDATED

Description

Transcript Variant: This variant (A) encodes the longer isoform (A).

Source sequence(s)

AC100153, AC132392, AC166834

Consensus CDS

CCDS57581.1

UniProtKB/Swiss-Prot

Q8BML1

Related

ENSMUSP00000047639.6, ENSMUST00000037991.12

Conserved Domains (3) summary

cd09439
Location:980 → 1034

LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)

cl30692
Location:87 → 224

UbiH; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases [Coenzyme transport and metabolism, Energy production and conversion]

pfam00307
Location:522 → 623

CH; Calponin homology (CH) domain
NM_001381862.1 → NP_001368791.1 [F-actin]-monooxygenase MICAL2 isoform D

Status: VALIDATED

Source sequence(s)

AC132392

Consensus CDS

CCDS52364.1

UniProtKB/TrEMBL

A0A1L1ST75

Related

ENSMUSP00000102256.2, ENSMUST00000106645.8

Conserved Domains (1) summary

pfam12130
Location:532 → 660

DUF3585; Protein of unknown function (DUF3585)
NM_001395864.1 → NP_001382793.1 [F-actin]-monooxygenase MICAL2 isoform C

Status: VALIDATED

Source sequence(s)

AC100153, AC132392, AC166834

UniProtKB/Swiss-Prot

B1B545, F6ZD54, F8WJF1, Q14DP1, Q3TR48, Q3UPU6, Q5DU17, Q8BML1, Q8CID1, Q9D5U9

Conserved Domains (5) summary

cd09439
Location:753 → 807

LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)

smart00033
Location:520 → 617

CH; Calponin homology domain

cl30692
Location:87 → 224

UbiH; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases [Coenzyme transport and metabolism, Energy production and conversion]

pfam09731
Location:1295 → 1670

Mitofilin; Mitochondrial inner membrane protein

pfam17380
Location:1514 → 1667

DUF5401; Family of unknown function (DUF5401)
NM_177282.5 → NP_796256.1 [F-actin]-monooxygenase MICAL2 isoform B

See identical proteins and their annotated locations for NP_796256.1

Status: VALIDATED

Description

Transcript Variant: This variant (B) lacks 5 alternate exons, resulting in the loss of an in-frame segment in the 3' coding region, and uses an alternate splice site in the 3' coding region, compared to variant 1. The resulting protein (isoform B) is shorter and has a distinct C-terminus, compared to isoform A.

Source sequence(s)

AC132392, AK030608, BB662540

Consensus CDS

CCDS21753.1

UniProtKB/Swiss-Prot

Q8BML1

Related

ENSMUSP00000051163.6, ENSMUST00000050149.12

Conserved Domains (4) summary

cd09439
Location:753 → 807

LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)

pfam00307
Location:522 → 617

CH; Calponin homology (CH) domain

pfam01494
Location:87 → 274

FAD_binding_3; FAD binding domain

cl21454
Location:91 → 121

NADB_Rossmann; Rossmann-fold NAD(P)(+)-binding proteins

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000073.7 Reference GRCm39 C57BL/6J

Range

111825032..112012311

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006507954.2 → XP_006508017.1 F-actin-monooxygenase MICAL2 isoform X1

See identical proteins and their annotated locations for XP_006508017.1

UniProtKB/Swiss-Prot

Q8BML1

Conserved Domains (3) summary

cd09439
Location:980 → 1034

LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)

cl30692
Location:87 → 224

UbiH; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases [Coenzyme transport and metabolism, Energy production and conversion]

pfam00307
Location:522 → 623

CH; Calponin homology (CH) domain
XM_036153171.1 → XP_036009064.1 F-actin-monooxygenase MICAL2 isoform X2

Conserved Domains (3) summary

cd09439
Location:938 → 992

LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)

pfam01494
Location:87 → 274

FAD_binding_3; FAD binding domain

cd21250
Location:514 → 623

CH_MICAL2; calponin homology (CH) domain found in molecule interacting with CasL protein 2
XM_036153172.1 → XP_036009065.1 F-actin-monooxygenase MICAL2 isoform X3

Conserved Domains (3) summary

cd09439
Location:873 → 927

LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)

pfam01494
Location:87 → 274

FAD_binding_3; FAD binding domain

cd21250
Location:514 → 623

CH_MICAL2; calponin homology (CH) domain found in molecule interacting with CasL protein 2
XM_036153173.1 → XP_036009066.1 F-actin-monooxygenase MICAL2 isoform X4

Conserved Domains (3) summary

cd09439
Location:831 → 885

LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)

pfam01494
Location:87 → 274

FAD_binding_3; FAD binding domain

cd21250
Location:514 → 623

CH_MICAL2; calponin homology (CH) domain found in molecule interacting with CasL protein 2
XM_036153174.1 → XP_036009067.1 F-actin-monooxygenase MICAL2 isoform X5

Conserved Domains (3) summary

cd09439
Location:789 → 843

LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)

pfam01494
Location:87 → 274

FAD_binding_3; FAD binding domain

cd21250
Location:514 → 623

CH_MICAL2; calponin homology (CH) domain found in molecule interacting with CasL protein 2
XM_030242685.1 → XP_030098545.1 F-actin-monooxygenase MICAL2 isoform X6

Conserved Domains (3) summary

cd09439
Location:753 → 807

LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)

pfam01494
Location:87 → 274

FAD_binding_3; FAD binding domain

cd21250
Location:514 → 623

CH_MICAL2; calponin homology (CH) domain found in molecule interacting with CasL protein 2
XM_036153175.1 → XP_036009068.1 F-actin-monooxygenase MICAL2 isoform X6

Conserved Domains (3) summary

cd09439
Location:753 → 807

LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)

pfam01494
Location:87 → 274

FAD_binding_3; FAD binding domain

cd21250
Location:514 → 623

CH_MICAL2; calponin homology (CH) domain found in molecule interacting with CasL protein 2