Tssk4 testis-specific serine kinase 4 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Tssk4provided by MGI
Official Full Name: testis-specific serine kinase 4provided by MGI
Primary source: MGI:MGI:1918349
See related: Ensembl:ENSMUSG00000007591 AllianceGenome:MGI:1918349
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: TSK-4; TSSK-4; 1700020B19Rik; 4933424F08Rik
Summary: Enables magnesium ion binding activity; protein homodimerization activity; and protein serine/threonine kinase activity. Involved in several processes, including flagellated sperm motility; positive regulation of CREB transcription factor activity; and protein phosphorylation. Located in sperm flagellum. Orthologous to human TSSK4 (testis specific serine kinase 4). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in testis adult (RPKM 29.3) and genital fat pad adult (RPKM 1.3) See more
Orthologs: human all
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Genomic context

Location:: 14 C3; 14 28.19 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	14	NC_000080.7 (55880893..55889996)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	14	NC_000080.6 (55643651..55652539)

Chromosome 14 - NC_000080.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Testis-specific serine/threonine protein kinase 4 (Tssk4) phosphorylates Odf2 at Ser-76
Title: Testis-specific serine/threonine protein kinase 4 (Tssk4) phosphorylates Odf2 at Ser-76.
Phosphorylated Tssk4 might participate in testis genes expressions by phosphorylating Crem at Ser-117.
Title: Phosphorylated testis-specific serine/threonine kinase 4 may phosphorylate Crem at Ser-117.
findings reveal that Tssk4 is required for maintaining the structural integrity of sperm flagellum and male fertility.
Title: Tssk4 is essential for maintaining the structural integrity of sperm flagellum.
HSP90 function abolished the catalytic activities of TSSK4 and -6 but did not significantly alter the specific activities of TSSK1 and -2. Inhibition of HSP90 resulted in increased TSSK ubiquitination and proteasomal degradation
Title: Heat shock protein 90 functions to stabilize and activate the testis-specific serine/threonine kinases, a family of kinases essential for male fertility.
autophosphorylation at Thr-197 plays a critical role in maintaining Tssk4 kinase activity, which might be involved in spermiogenesis.
Title: Testis specific serine/threonine kinase 4 (Tssk4) maintains its kinase activity by phosphorylating itself at Thr-197.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (1) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:493966

NoName (e-PCR)
- UniSTS:490696

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables magnesium ion binding	IDA Inferred from Direct Assay more info	PubMed
enables magnesium ion binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed
NOT enables protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables protein-containing complex binding	IPI Inferred from Physical Interaction more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in fertilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in flagellated sperm motility	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within multicellular organism development	IEA Inferred from Electronic Annotation more info
involved_in peptidyl-serine phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in peptidyl-serine phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in peptidyl-threonine autophosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of CREB transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of CREB transcription factor activity	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in spermatid development	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within spermatogenesis	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in acrosomal vesicle	TAS Traceable Author Statement more info	PubMed
located_in cell projection	IEA Inferred from Electronic Annotation more info
located_in cilium	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
located_in motile cilium	IDA Inferred from Direct Assay more info	PubMed
located_in sperm flagellum	IMP Inferred from Mutant Phenotype more info	PubMed

General protein information

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Preferred Names: testis-specific serine/threonine-protein kinase 4

Names: testis-specific kinase 4; testis-specific serine/threonine protein kinase 5 variant alpha; testis-specific serine/threonine protein kinase 5 variant beta; testis-specific serine/threonine protein kinase 5 variant delta; testis-specific serine/threonine protein kinase 5 variant gamma

NP_001240817.1

EC 2.7.11.1

NP_001240818.1

EC 2.7.11.1

NP_081949.1

EC 2.7.11.1

XP_006519614.1

EC 2.7.11.1

XP_006519615.1

EC 2.7.11.1

XP_036014742.1

EC 2.7.11.1

XP_036014743.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001253888.1 → NP_001240817.1 testis-specific serine/threonine-protein kinase 4 isoform 3

See identical proteins and their annotated locations for NP_001240817.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a longer protein (isoform 3), compared to isoform 1.

Source sequence(s)

AC174678

Consensus CDS

CCDS88669.1

UniProtKB/TrEMBL

A0A2I3BQS0

Related

ENSMUSP00000154783.2, ENSMUST00000228395.2

Conserved Domains (2) summary

smart00220
Location:25 → 303

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14162
Location:24 → 302

STKc_TSSK4-like; Catalytic domain of testis-specific serine/threonine kinase 4 and similar proteins
NM_001253889.1 → NP_001240818.1 testis-specific serine/threonine-protein kinase 4 isoform 4

See identical proteins and their annotated locations for NP_001240818.1

Status: VALIDATED

Description

Transcript Variant: This variant (4) uses two alternate in-frame splice sites in the coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1.

Source sequence(s)

AC174678, AK006144, AK016890

Consensus CDS

CCDS56959.1

UniProtKB/TrEMBL

A0A2I3BQS0

Related

ENSMUSP00000007735.9, ENSMUST00000007735.9

Conserved Domains (2) summary

smart00220
Location:25 → 278

S_TKc; Serine/Threonine protein kinases, catalytic domain

cl21453
Location:24 → 278

PKc_like; Protein Kinases, catalytic domain
NM_027673.3 → NP_081949.1 testis-specific serine/threonine-protein kinase 4 isoform 1

See identical proteins and their annotated locations for NP_081949.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes isoform (1).

Source sequence(s)

AC174678, AK016890

Consensus CDS

CCDS88670.1

UniProtKB/Swiss-Prot

A3QQQ9, A3QQR0, A3QQR1, A9P6P7, A9P6P9, Q9D411, Q9DA58

UniProtKB/TrEMBL

A0A2I3BQE9

Related

ENSMUSP00000153752.2, ENSMUST00000226591.2

Conserved Domains (2) summary

smart00220
Location:25 → 293

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14162
Location:24 → 292

STKc_TSSK4-like; Catalytic domain of testis-specific serine/threonine kinase 4 and similar proteins

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000080.7 Reference GRCm39 C57BL/6J

Range

55880893..55889996

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036158850.1 → XP_036014743.1 testis-specific serine/threonine-protein kinase 4 isoform X4

Conserved Domains (1) summary

PHA03247
Location:12 → 113

PHA03247; large tegument protein UL36; Provisional
XM_006519551.4 → XP_006519614.1 testis-specific serine/threonine-protein kinase 4 isoform X1

Conserved Domains (1) summary

cl21453
Location:3 → 230

PKc_like; Protein Kinases, catalytic domain
XM_036158849.1 → XP_036014742.1 testis-specific serine/threonine-protein kinase 4 isoform X3

UniProtKB/TrEMBL

A0A2I3BQM6

Conserved Domains (1) summary

cl21453
Location:24 → 149

PKc_like; Protein Kinases, catalytic domain
XM_006519552.1 → XP_006519615.1 testis-specific serine/threonine-protein kinase 4 isoform X2

Conserved Domains (1) summary

cl21453
Location:46 → 191

PKc_like; Protein Kinases, catalytic domain