Sucla2 succinate-Coenzyme A ligase, ADP-forming, beta subunit [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Sucla2provided by MGI
Official Full Name: succinate-Coenzyme A ligase, ADP-forming, beta subunitprovided by MGI
Primary source: MGI:MGI:1306775
See related: Ensembl:ENSMUSG00000022110 AllianceGenome:MGI:1306775
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: A-SCS; SCS-betaA; 4930547K18Rik
Summary: Predicted to enable succinate-CoA ligase (ADP-forming) activity and succinate-CoA ligase (GDP-forming) activity. Predicted to be involved in succinate metabolic process; succinyl-CoA metabolic process; and tricarboxylic acid cycle. Located in mitochondrion and myelin sheath. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; respiratory system; and sensory organ. Used to study mitochondrial DNA depletion syndrome 5. Human ortholog(s) of this gene implicated in mitochondrial DNA depletion syndrome 5. Orthologous to human SUCLA2 (succinate-CoA ligase ADP-forming subunit beta). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in heart adult (RPKM 50.4), testis adult (RPKM 42.5) and 26 other tissues See more
Orthologs: human all
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Genomic context

Location:: 14 D3; 14 39.06 cM

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	14	NC_000080.7 (73790226..73833584)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	14	NC_000080.6 (73552786..73596144)

Chromosome 14 - NC_000080.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

SUCLA2-coupled regulation of GLS succinylation and activity counteracts oxidative stress in tumor cells.
Title: SUCLA2-coupled regulation of GLS succinylation and activity counteracts oxidative stress in tumor cells.
SCS A-beta loss mediates severe mitochondrial dysfunction including compromised OXPHOS efficiency, increased ROS generation, mtDNA depletion as well as altered mitochondrial dynamics towards fusion.
Title: Loss of succinyl-CoA synthase ADP-forming β subunit disrupts mtDNA stability and mitochondrial dynamics in neurons.
Sucla2(SAbetageo) homozygotes exhibited recessive lethality, with most mutants dying late in gestation (e18.5). Mutant placenta and embryonic (e17.5) brain, heart and muscle showed varying degrees of mitochondrial DNA depletion.
Title: Screen for abnormal mitochondrial phenotypes in mouse embryonic stem cells identifies a model for succinyl-CoA ligase deficiency and mtDNA depletion.
ADP and GDP succinyl-CoA synthetases have different expression patterns and specificity
Title: Expression of two succinyl-CoA synthetases with different nucleotide specificities in mammalian tissues.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (2) 1 citation
Endonuclease-mediated (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Sucla2 (e-PCR), detects polymorphism
- UniSTS:507251

RH141159 (e-PCR)
- UniSTS:223155

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables catalytic activity	IEA Inferred from Electronic Annotation more info
enables ligase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables succinate-CoA ligase (ADP-forming) activity	IBA Inferred from Biological aspect of Ancestor more info
enables succinate-CoA ligase (ADP-forming) activity	ISO Inferred from Sequence Orthology more info
enables succinate-CoA ligase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed

Process	Evidence Code	Pubs
involved_in succinate metabolic process	ISO Inferred from Sequence Orthology more info
involved_in succinyl-CoA metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in succinyl-CoA metabolic process	ISO Inferred from Sequence Orthology more info
involved_in tricarboxylic acid cycle	IBA Inferred from Biological aspect of Ancestor more info
involved_in tricarboxylic acid cycle	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in mitochondrion	HDA more info	PubMed
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
located_in myelin sheath	HDA more info	PubMed
part_of succinate-CoA ligase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of succinate-CoA ligase complex (ADP-forming)	IMP Inferred from Mutant Phenotype more info	PubMed

General protein information

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Preferred Names: succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial

Names: ATP-specific succinyl-CoA synthetase subunit beta; succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial; succinyl-CoA synthetase beta-A chain

NP_001348567.1

EC 6.2.1.5

NP_035636.1

EC 6.2.1.5

XP_006518820.1

EC 6.2.1.5

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001361638.1 → NP_001348567.1 succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial isoform 2

Status: VALIDATED

Source sequence(s)

AC113198, AC155246

Conserved Domains (1) summary

cl25712
Location:1 → 311

SucC; Succinyl-CoA synthetase, beta subunit [Energy production and conversion]
NM_011506.4 → NP_035636.1 succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial isoform 1 precursor

See identical proteins and their annotated locations for NP_035636.1

Status: VALIDATED

Source sequence(s)

AC113198, AC155246

Consensus CDS

CCDS27271.1

UniProtKB/Swiss-Prot

Q3TVH1, Q8BGS6, Q9Z2I9

UniProtKB/TrEMBL

Q3UCC6

Related

ENSMUSP00000123765.2, ENSMUST00000160507.8

Conserved Domains (3) summary

PRK00696
Location:55 → 445

sucC; succinyl-CoA synthetase subunit beta; Provisional

pfam00549
Location:321 → 441

Ligase_CoA; CoA-ligase

pfam08442
Location:55 → 262

ATP-grasp_2; ATP-grasp domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000080.7 Reference GRCm39 C57BL/6J

Range

73790226..73833584

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006518757.4 → XP_006518820.1 succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial isoform X1

UniProtKB/TrEMBL

Q3UCC6

Conserved Domains (1) summary

PRK00696
Location:92 → 482

sucC; ADP-forming succinate--CoA ligase subunit beta