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    Tmem176b transmembrane protein 176B [ Mus musculus (house mouse) ]

    Gene ID: 65963, updated on 21-Apr-2024

    Summary

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    Official Symbol
    Tmem176bprovided by MGI
    Official Full Name
    transmembrane protein 176Bprovided by MGI
    Primary source
    MGI:MGI:1916348
    See related
    Ensembl:ENSMUSG00000029810 AllianceGenome:MGI:1916348
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Lr8; Clast1; 1810009M01Rik
    Summary
    Acts upstream of or within negative regulation of dendritic cell differentiation. Predicted to be located in membrane and nucleus. Predicted to be integral component of membrane. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; respiratory system; and sensory organ. Orthologous to human TMEM176B (transmembrane protein 176B). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Broad expression in kidney adult (RPKM 289.3), adrenal adult (RPKM 166.2) and 22 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    6 B2.3; 6 23.75 cM
    Exon count:
    11
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 6 NC_000072.7 (48810745..48818363, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 6 NC_000072.6 (48833811..48845948, complement)

    Chromosome 6 - NC_000072.7Genomic Context describing neighboring genes Neighboring gene predicted gene, 30781 Neighboring gene STARR-positive B cell enhancer ABC_E10379 Neighboring gene septin 7 pseudogene Neighboring gene transmembrane protein 176A Neighboring gene predicted gene 44764 Neighboring gene predicted gene 7932

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Oncogenic Ras-mediated downregulation of Clast1/LR8 is involved in Ras-mediated neoplastic transformation and tumorigenesis in NIH3T3 cells. Ryu SH, et al. Cancer Sci, 2010 Sep. PMID 20550525
    2. Generation of a Double KO Mouse by Simultaneous Targeting of the Neighboring Genes Tmem176a and Tmem176b Using CRISPR/Cas9: Key Steps from Design to Genotyping. Lemoine A, et al. J Genet Genomics, 2016 May 20. PMID 27234594
    3. Autologous dendritic cells prolong allograft survival through Tmem176b-dependent antigen cross-presentation. Segovia M, et al. Am J Transplant, 2014 May. PMID 24731243, Free PMC Article
    4. A novel factor, Tmem176b, induced by activin-like kinase 2 signal promotes the differentiation of myoblasts into osteoblasts. Yano M, et al. Exp Clin Endocrinol Diabetes, 2014 Jan. PMID 24085390
    5. Dendritic Cells Require TMEM176A/B Ion Channels for Optimal MHC Class II Antigen Presentation to Naive CD4(+) T Cells. Lancien M, et al. J Immunol, 2021 Jul 15. PMID 34233909

    See all (37) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Dendritic Cells Require TMEM176A/B Ion Channels for Optimal MHC Class II Antigen Presentation to Naive CD4(+) T Cells.
      Title: Dendritic Cells Require TMEM176A/B Ion Channels for Optimal MHC Class II Antigen Presentation to Naive CD4(+) T Cells.
    2. myogenic differentiation is achieved through the specific interaction between Cry2 and Bclaf1, which stabilizes mRNAs encoding cyclin D1, a G1/S phase transition regulator, and Tmem176b, a transmembrane regulator for myogenic cell fusion.
      Title: Cry2 Is Critical for Circadian Regulation of Myogenic Differentiation by Bclaf1-Mediated mRNA Stabilization of Cyclin D1 and Tmem176b.
    3. Study characterized Tmem176a and b expression in RORgamma t+ lymphocytes at transcriptional and protein levels and present evidence that both genes exert a redundant ion channel function related to a colocalisation in close proximity to the Golgi apparatus.
      Title: RORγt+ cells selectively express redundant cation channels linked to the Golgi apparatus.
    4. Tmem176b induces the differentiation of myoblasts into an osteoblast lineage.
      Title: A novel factor, Tmem176b, induced by activin-like kinase 2 signal promotes the differentiation of myoblasts into osteoblasts.
    5. data identify the downregulation of Clast1/LR8 as a potentially important mechanism by which oncogenic Ras-mediated neoplastic transformation occurs
      Title: Oncogenic Ras-mediated downregulation of Clast1/LR8 is involved in Ras-mediated neoplastic transformation and tumorigenesis in NIH3T3 cells.
    6. These data suggest that Tmem176B and Tmem176A associate to form multimers and restrain dendritic cell maturation.
      Title: Tmem176B and Tmem176A are associated with the immature state of dendritic cells.
    7. The Clast1 gene is ubiquitously expressed in various organs of adult mice and is required for development of cerebellar granule cells.
      Title: Role of Clast1 in development of cerebellar granule cells.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Targeted (4)  1 citation
    • Endonuclease-mediated (2) 

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within cell differentiation IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of dendritic cell differentiation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of dendritic cell differentiation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within negative regulation of dendritic cell differentiation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    transmembrane protein 176B

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001164207.1 → NP_001157679.1  transmembrane protein 176B

      See identical proteins and their annotated locations for NP_001157679.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 through 6 encode the same protein.
      Source sequence(s)
      AC115045, AK007408, BE457679, DV047496
      Consensus CDS
      CCDS20116.1
      UniProtKB/Swiss-Prot
      Q9R1Q6, Q9WU52
      Related
      ENSMUSP00000131064.2, ENSMUST00000166247.8
      Conserved Domains (1) summary
      pfam04103
      Location:60 → 224
      CD20; CD20-like family

    2. NM_001164208.1 → NP_001157680.1  transmembrane protein 176B

      See identical proteins and their annotated locations for NP_001157680.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1 through 6 encode the same protein.
      Source sequence(s)
      AC115045, AK007408, BE457679, BQ571258, DV047496
      Consensus CDS
      CCDS20116.1
      UniProtKB/Swiss-Prot
      Q9R1Q6, Q9WU52
      Conserved Domains (1) summary
      pfam04103
      Location:60 → 224
      CD20; CD20-like family

    3. NM_001164209.2 → NP_001157681.1  transmembrane protein 176B

      See identical proteins and their annotated locations for NP_001157681.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1 through 6 encode the same protein.
      Source sequence(s)
      AK007408, BE457679, CX213755, CX223561, DV047496
      Consensus CDS
      CCDS20116.1
      UniProtKB/Swiss-Prot
      Q9R1Q6, Q9WU52
      Related
      ENSMUSP00000098972.5, ENSMUST00000101429.11
      Conserved Domains (1) summary
      pfam04103
      Location:60 → 224
      CD20; CD20-like family

    4. NM_001286651.1 → NP_001273580.1  transmembrane protein 176B

      See identical proteins and their annotated locations for NP_001273580.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (5) differs in the 5' UTR compared to variant 1. Variants 1 through 6 encode the same protein.
      Source sequence(s)
      AK007408, BE457679, CX213755, CX223561, DV047496
      Consensus CDS
      CCDS20116.1
      UniProtKB/Swiss-Prot
      Q9R1Q6, Q9WU52
      Conserved Domains (1) summary
      pfam04103
      Location:60 → 224
      CD20; CD20-like family

    5. NM_001286652.1 → NP_001273581.1  transmembrane protein 176B

      See identical proteins and their annotated locations for NP_001273581.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (6) differs in the 5' UTR compared to variant 1. Variants 1 through 6 encode the same protein.
      Source sequence(s)
      AK007408, BE457679, CX213755, CX223561, DV059241
      Consensus CDS
      CCDS20116.1
      UniProtKB/Swiss-Prot
      Q9R1Q6, Q9WU52
      Related
      ENSMUSP00000144864.2, ENSMUST00000204073.3
      Conserved Domains (1) summary
      pfam04103
      Location:60 → 224
      CD20; CD20-like family

    6. NM_023056.4 → NP_075543.1  transmembrane protein 176B

      See identical proteins and their annotated locations for NP_075543.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) represents the longest transcript. Variants 1 through 6 encode the same protein.
      Source sequence(s)
      AB031386, AC115045, BE457679
      Consensus CDS
      CCDS20116.1
      UniProtKB/Swiss-Prot
      Q9R1Q6, Q9WU52
      Related
      ENSMUSP00000128705.2, ENSMUST00000164733.4
      Conserved Domains (1) summary
      pfam04103
      Location:60 → 224
      CD20; CD20-like family

    RNA

    1. NR_104575.1 RNA Sequence

      Status: VALIDATED

      Description
      Transcript Variant: This variant (7) differs in the 5' terminal exon and lacks an exon in the 5' region, compared to variant 1. This variant is represented as non-coding because it lacks a large portion of the coding region, including the translational start codon, found in variant 1.
      Source sequence(s)
      AK007408, BE457679, CX213755, CX223561, DV047496

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000072.7 Reference GRCm39 C57BL/6J

      Range
      48810745..48818363 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_036152260.1 → XP_036008153.1  transmembrane protein 176B isoform X1

      UniProtKB/Swiss-Prot
      Q9R1Q6, Q9WU52
      Conserved Domains (1) summary
      pfam04103
      Location:60 → 224
      CD20; CD20-like family

    2. XM_006506459.5 → XP_006506522.1  transmembrane protein 176B isoform X1

      See identical proteins and their annotated locations for XP_006506522.1

      UniProtKB/Swiss-Prot
      Q9R1Q6, Q9WU52
      Conserved Domains (1) summary
      pfam04103
      Location:60 → 224
      CD20; CD20-like family

    3. XM_030255531.2 → XP_030111391.1  transmembrane protein 176B isoform X1

      UniProtKB/Swiss-Prot
      Q9R1Q6, Q9WU52
      Conserved Domains (1) summary
      pfam04103
      Location:60 → 224
      CD20; CD20-like family

    4. XM_006506458.4 → XP_006506521.1  transmembrane protein 176B isoform X1

      See identical proteins and their annotated locations for XP_006506521.1

      UniProtKB/Swiss-Prot
      Q9R1Q6, Q9WU52
      Conserved Domains (1) summary
      pfam04103
      Location:60 → 224
      CD20; CD20-like family

    5. XM_006506457.5 → XP_006506520.1  transmembrane protein 176B isoform X1

      See identical proteins and their annotated locations for XP_006506520.1

      UniProtKB/Swiss-Prot
      Q9R1Q6, Q9WU52
      Conserved Domains (1) summary
      pfam04103
      Location:60 → 224
      CD20; CD20-like family

    6. XM_030255532.2 → XP_030111392.1  transmembrane protein 176B isoform X1

      UniProtKB/Swiss-Prot
      Q9R1Q6, Q9WU52
      Related
      ENSMUSP00000145395.2, ENSMUST00000203355.3
      Conserved Domains (1) summary
      pfam04103
      Location:60 → 224
      CD20; CD20-like family

    7. XM_030255534.2 → XP_030111394.1  transmembrane protein 176B isoform X1

      UniProtKB/Swiss-Prot
      Q9R1Q6, Q9WU52
      Conserved Domains (1) summary
      pfam04103
      Location:60 → 224
      CD20; CD20-like family

    8. XM_006506456.5 → XP_006506519.1  transmembrane protein 176B isoform X1

      See identical proteins and their annotated locations for XP_006506519.1

      UniProtKB/Swiss-Prot
      Q9R1Q6, Q9WU52
      Conserved Domains (1) summary
      pfam04103
      Location:60 → 224
      CD20; CD20-like family

    9. XM_017321710.3 → XP_017177199.1  transmembrane protein 176B isoform X1

      UniProtKB/Swiss-Prot
      Q9R1Q6, Q9WU52
      Conserved Domains (1) summary
      pfam04103
      Location:60 → 224
      CD20; CD20-like family

    10. XM_006506460.5 → XP_006506523.1  transmembrane protein 176B isoform X1

      See identical proteins and their annotated locations for XP_006506523.1

      UniProtKB/Swiss-Prot
      Q9R1Q6, Q9WU52
      Conserved Domains (1) summary
      pfam04103
      Location:60 → 224
      CD20; CD20-like family

    11. XM_030255533.2 → XP_030111393.1  transmembrane protein 176B isoform X1

      UniProtKB/Swiss-Prot
      Q9R1Q6, Q9WU52
      Conserved Domains (1) summary
      pfam04103
      Location:60 → 224
      CD20; CD20-like family

    12. XM_030255535.2 → XP_030111395.1  transmembrane protein 176B isoform X1

      UniProtKB/Swiss-Prot
      Q9R1Q6, Q9WU52
      Conserved Domains (1) summary
      pfam04103
      Location:60 → 224
      CD20; CD20-like family
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9R1Q6.1 GenPept UniProtKB/Swiss-Prot:Q9R1Q6

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