Dnaja1 DnaJ heat shock protein family (Hsp40) member A1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Dnaja1provided by MGI
Official Full Name: DnaJ heat shock protein family (Hsp40) member A1provided by MGI
Primary source: MGI:MGI:1270129
See related: Ensembl:ENSMUSG00000028410 AllianceGenome:MGI:1270129
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Hsj2; HSJ-2; Nedd7
Summary: The protein encoded by this gene is a member of the DnaJ family, whose members act as cochaperones of heat shock protein 70. Heat shock proteins facilitate protein folding, trafficking, prevention of aggregation, and proteolytic degradation. Members of this family are characterized by a highly conserved N-terminal J domain, a glycine/phenylalanine-rich region, four CxxCxGxG zinc finger repeats, and a C-terminal substrate-binding domain. The J domain mediates the interaction with heat shock protein 70 to recruit substrates and regulate ATP hydrolysis activity. Mice deficient for this gene display reduced levels of activation‐induced deaminase, an enzyme that deaminates deoxycytidine at the immunoglobulin genes during immune responses. In addition, mice lacking this gene exhibit severe defects in spermatogenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Expression: Broad expression in CNS E11.5 (RPKM 48.9), CNS E14 (RPKM 41.5) and 19 other tissues See more
Orthologs: human all
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Genomic context

Location:: 4 A5; 4 20.46 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	4	NC_000070.7 (40722468..40734965)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	4	NC_000070.6 (40722468..40734965)

Chromosome 4 - NC_000070.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Cereblon Regulates the Proteotoxicity of Tau by Tuning the Chaperone Activity of DNAJA1.
Title: Cereblon Regulates the Proteotoxicity of Tau by Tuning the Chaperone Activity of DNAJA1.
Identification of a druggable protein-protein interaction site between mutant p53 and its stabilizing chaperone DNAJA1.
Title: Identification of a druggable protein-protein interaction site between mutant p53 and its stabilizing chaperone DNAJA1.
Our results show that farnesylated DNAJA1 is a crucial chaperone in maintaining mutant p53 stabilization and targeting farnesylated DNAJA1 by atorvastatin will be critical for inhibiting p53 mutant cancer.
Title: Inhibition of mutant Kras and p53-driven pancreatic carcinogenesis by atorvastatin: Mainly via targeting of the farnesylated DNAJA1 in chaperoning mutant p53.
DnaJa1 farnesylation is required to maintain, and farnesyltransferase inhibition reduces, AID protein levels in B cells. Thus, DnaJa1 is a limiting factor that plays a non-redundant role in the functional stabilization of AID
Title: Optimal functional levels of activation-induced deaminase specifically require the Hsp40 DnaJa1.
Hsc70 and a dimer of DjA1 independently bind to an unfolded protein
Title: Multiple molecules of Hsc70 and a dimer of DjA1 independently bind to an unfolded protein.
HSP40 binding is the first step in the HSP90 chaperoning pathway for the progesterone receptor
Title: HSP40 binding is the first step in the HSP90 chaperoning pathway for the progesterone receptor.
Results indicate that DjA1 and DjB4 of subfamilies A and B of human Hsp40 have different quaternary structures and chaperone functions.
Title: Low resolution structural study of two human HSP40 chaperones in solution. DJA1 from subfamily A and DJB4 from subfamily B have different quaternary structures.
DnaJ 1 protein expression is down regulated in the spinal cord and testis of wobbler mouse and precedes motor neuron degeneration.
Title: Early defect in the expression of mouse sperm DNAJ 1, a member of the DNAJ/heat shock protein 40 chaperone protein family, in the spinal cord of the wobbler mouse, a murine model of motoneuronal degeneration.
loss of DjA1 in led to severe defects in spermatogenesis that involve aberrant androgen signaling
Title: A type I DnaJ homolog, DjA1, regulates androgen receptor signaling and spermatogenesis.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (3) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH126529 (e-PCR)
- UniSTS:209669

Dnaja1 (e-PCR), detects polymorphism
- UniSTS:143408

PMC153017P1 (e-PCR)
- UniSTS:271247

AW556129 (e-PCR)
- UniSTS:185577

BB283300 (e-PCR)
- UniSTS:207611

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATPase activator activity	ISO Inferred from Sequence Orthology more info
enables C3HC4-type RING finger domain binding	ISO Inferred from Sequence Orthology more info
enables G protein-coupled receptor binding	ISO Inferred from Sequence Orthology more info
enables Hsp70 protein binding	ISO Inferred from Sequence Orthology more info
enables Tat protein binding	ISO Inferred from Sequence Orthology more info
enables heat shock protein binding	IEA Inferred from Electronic Annotation more info
enables low-density lipoprotein particle receptor binding	ISO Inferred from Sequence Orthology more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-folding chaperone binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein-folding chaperone binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info
enables unfolded protein binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within androgen receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within flagellated sperm motility	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of JUN kinase activity	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of establishment of protein localization to mitochondrion	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of nitrosative stress-induced intrinsic apoptotic signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of protein ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in protein folding	IEA Inferred from Electronic Annotation more info
involved_in protein localization to mitochondrion	ISO Inferred from Sequence Orthology more info
involved_in protein refolding	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of protein transport	ISO Inferred from Sequence Orthology more info
involved_in response to heat	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in intracellular membrane-bounded organelle	IEA Inferred from Electronic Annotation more info
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in microtubule cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: dnaJ homolog subfamily A member 1

Names: DnaJ (Hsp40) homolog, subfamily A, member 1; dnaJ protein homolog 2; heat shock 40 kDa protein 4; heat shock protein J2; heat shock protein, Dnaj-like 2; neural precursor cell expressed, developmentally down-regulated gene 7

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001164671.2 → NP_001158143.1 dnaJ homolog subfamily A member 1

See identical proteins and their annotated locations for NP_001158143.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein.

Source sequence(s)

AK083046, AK145963, BP766105, BY245852, CN669546

Consensus CDS

CCDS18049.1

UniProtKB/Swiss-Prot

P54102, P63037

UniProtKB/TrEMBL

Q3TK61, Q5NTY0

Related

ENSMUSP00000129730.2, ENSMUST00000164233.8

Conserved Domains (1) summary

PTZ00037
Location:2 → 394

PTZ00037; DnaJ_C chaperone protein; Provisional
NM_001164672.2 → NP_001158144.1 dnaJ homolog subfamily A member 1

See identical proteins and their annotated locations for NP_001158144.1

Status: REVIEWED

Description

Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein.

Source sequence(s)

AK083046, AK145963, BP766105, CK617936

Consensus CDS

CCDS18049.1

UniProtKB/Swiss-Prot

P54102, P63037

UniProtKB/TrEMBL

Q3TK61, Q5NTY0

Conserved Domains (1) summary

PTZ00037
Location:2 → 394

PTZ00037; DnaJ_C chaperone protein; Provisional
NM_008298.6 → NP_032324.1 dnaJ homolog subfamily A member 1

See identical proteins and their annotated locations for NP_032324.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein.

Source sequence(s)

AK083046, AK145963, BB574002, BP766105

Consensus CDS

CCDS18049.1

UniProtKB/Swiss-Prot

P54102, P63037

UniProtKB/TrEMBL

Q3TK61, Q5NTY0

Related

ENSMUSP00000030118.4, ENSMUST00000030118.10

Conserved Domains (1) summary

PTZ00037
Location:2 → 394

PTZ00037; DnaJ_C chaperone protein; Provisional