Pde8a phosphodiesterase 8A [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Pde8aprovided by MGI
Official Full Name: phosphodiesterase 8Aprovided by MGI
Primary source: MGI:MGI:1277116
See related: Ensembl:ENSMUSG00000025584 AllianceGenome:MGI:1277116
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Pde8
Summary: Enables 3',5'-cyclic-AMP phosphodiesterase activity. Involved in cellular response to epidermal growth factor stimulus and positive regulation of ERK1 and ERK2 cascade. Is expressed in several structures, including genitourinary system; gut gland; integumental system; nervous system; and skeleton. Orthologous to human PDE8A (phosphodiesterase 8A). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in testis adult (RPKM 48.2), kidney adult (RPKM 13.7) and 18 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7 D3; 7 45.71 cM

Exon count:: 23

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (80863013..80984283)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (81213265..81334535)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

our studies demonstrate that PDE8A inhibition by enzymatic inhibitors or PDE8A-Raf-1 kinase complex disruptors decreases Teff cell adhesion and migration under flow, and represents a novel approach to target T cells in inflammation
Title: PDE8 controls CD4(+) T cell motility through the PDE8A-Raf-1 kinase signaling complex.
findings suggest that both PDE8A and PDE8B play essential roles to maintain low cAMP levels, thereby suppressing resting steroidogenesis by keeping CEH/HSL inactive and StAR protein expression low
Title: cAMP-specific phosphodiesterases 8A and 8B, essential regulators of Leydig cell steroidogenesis.
PDE8 as a novel target for suppression of Teff cell functions, including adhesion to endothelial cells
Title: PDE8 regulates rapid Teff cell adhesion and proliferation independent of ICER.
PDE8A plays a critical role in the modulation of at least one compartment of cAMP and hence PKA activity during beta-adrenergic receptor (betaAR) activation in ventricular myocytes.
Title: Phosphodiesterase 8A (PDE8A) regulates excitation-contraction coupling in ventricular myocytes.
pharmacological manipulation of PDE8A, alone or in combination with other PDEs present in Leydig cells, may be exploited to modulate testosterone synthesis
Title: Modulation of Leydig cell function by cyclic nucleotide phosphodiesterase 8A.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (3) 1 citation
Endonuclease-mediated (3)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Pde8a (e-PCR), detects polymorphism
- UniSTS:498437

RH136499 (e-PCR)
- UniSTS:210482

RH140728 (e-PCR)
- UniSTS:222794

AF067806 (e-PCR)
- UniSTS:163422

RH143306 (e-PCR)
- UniSTS:232553

21.MMHAP17FRG9.seq (e-PCR)
- UniSTS:123510

AI551852 (e-PCR)
- UniSTS:179597

Pde8a (e-PCR), detects polymorphism
- UniSTS:498350

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 3',5'-cyclic-AMP phosphodiesterase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 3',5'-cyclic-AMP phosphodiesterase activity	IDA Inferred from Direct Assay more info	PubMed
enables 3',5'-cyclic-AMP phosphodiesterase activity	ISO Inferred from Sequence Orthology more info
enables 3',5'-cyclic-GMP phosphodiesterase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 3',5'-cyclic-GMP phosphodiesterase activity	ISO Inferred from Sequence Orthology more info
enables 3',5'-cyclic-nucleotide phosphodiesterase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables kinase binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables phosphoric diester hydrolase activity	IEA Inferred from Electronic Annotation more info
enables protein kinase activator activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in cAMP-mediated signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to epidermal growth factor stimulus	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to epidermal growth factor stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to epidermal growth factor stimulus	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of ERK1 and ERK2 cascade	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of ERK1 and ERK2 cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of ERK1 and ERK2 cascade	ISO Inferred from Sequence Orthology more info
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
is_active_in perinuclear region of cytoplasm	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A

NP_032829.1

EC 3.1.4.53

XP_036008663.1

EC 3.1.4.53

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_008803.3 → NP_032829.1 high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A

See identical proteins and their annotated locations for NP_032829.1

Status: VALIDATED

Source sequence(s)

AF067806, AK148238, AV272936, BC125578, CO808457

Consensus CDS

CCDS40005.1

UniProtKB/Swiss-Prot

O88502, Q059P6

UniProtKB/TrEMBL

Q3UFX9

Related

ENSMUSP00000026672.8, ENSMUST00000026672.8

Conserved Domains (5) summary

COG0745
Location:78 → 155

OmpR; DNA-binding response regulator, OmpR family, contains REC and winged-helix (wHTH) domain [Signal transduction mechanisms, Transcription]

cd00130
Location:226 → 320

PAS; PAS domain; PAS motifs appear in archaea, eubacteria and eukarya. Probably the most surprising identification of a PAS domain was that in EAG-like K+-channels. PAS domains have been found to bind ligands, and to act as sensors for light and oxygen in ...

pfam00233
Location:550 → 801

PDEase_I; 3'5'-cyclic nucleotide phosphodiesterase

pfam13426
Location:222 → 320

PAS_9; PAS domain

cl19078
Location:79 → 165

REC; Signal receiver domain; originally thought to be unique to bacteria (CheY, OmpR, NtrC, and PhoB), now recently identified in eukaroytes ETR1 Arabidopsis thaliana; this domain receives the signal from the sensor partner in a two-component systems; ...

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000073.7 Reference GRCm39 C57BL/6J

Range

80863013..80984283

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036152770.1 → XP_036008663.1 high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A isoform X1

Conserved Domains (4) summary

COG0745
Location:78 → 155

OmpR; DNA-binding response regulator, OmpR family, contains REC and winged-helix (wHTH) domain [Signal transduction mechanisms, Transcription]

COG2202
Location:118 → 320

PAS; PAS domain [Signal transduction mechanisms]

pfam13426
Location:222 → 320

PAS_9; PAS domain

cl21469
Location:550 → 574

HDc; Metal dependent phosphohydrolases with conserved 'HD' motif