Myo9b myosin IXb [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Myo9bprovided by RGD
Official Full Name: myosin IXbprovided by RGD
Primary source: RGD:3146
See related: Ensembl:ENSRNOG00000016256 AllianceGenome:RGD:3146
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables several functions, including ATP hydrolysis activity; ATP-dependent protein binding activity; and adenyl ribonucleotide binding activity. Involved in Rho protein signal transduction. Located in several cellular components, including actin filament; lamellipodium; and ruffle. Human ortholog(s) of this gene implicated in celiac disease. Orthologous to human MYO9B (myosin IXB). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Spleen (RPKM 267.1), Thymus (RPKM 233.5) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 16p14

Exon count:: 43

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	16	NC_086034.1 (17979374..18064100)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	16	NC_051351.1 (17945379..18030114)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	16	NC_005115.4 (19669373..19754065)

Chromosome 16 - NC_086034.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

data demonstrate that Myo9b and RICS repress RhoA/Rock signaling and modulate dendritic morphogenesis in cortical neurons
Title: Myo9b and RICS modulate dendritic morphology of cortical neurons.
the extended loop 2 in Myo9b binds F-actin and influences the binding of the conventional stereo-specific actin-binding site.
Title: Functional role of the extended loop 2 in the myosin 9b head for binding F-actin.
myosin IXb binds to BIG1, which regulates its Rho-GTPase activating protein activity
Title: BIG1 is a binding partner of myosin IXb and regulates its Rho-GTPase activating protein activity.
Unlike other processive myosins, Myo9b exhibited a low affinity for ADP, and ADP release was not rate-limiting in the ATPase cycle.
Title: Kinetic mechanism of myosin IXB and the contributions of two class IX-specific regions.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

UniSTS:259375 (e-PCR)
- UniSTS:259375

Myo9b (e-PCR), detects polymorphism
- UniSTS:259375

UniSTS:259378 (e-PCR)
- UniSTS:259378

Myo9b (e-PCR), detects polymorphism
- UniSTS:259378

Myo9b (e-PCR), detects polymorphism
- UniSTS:259379

AI237607 (e-PCR)
- UniSTS:250688

RH94693 (e-PCR)
- UniSTS:119940

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ADP binding	IDA Inferred from Direct Assay more info	PubMed
enables ADP binding	IEA Inferred from Electronic Annotation more info
enables ADP binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables ADP binding	ISO Inferred from Sequence Orthology more info
enables ATP binding	IBA Inferred from Biological aspect of Ancestor more info
enables ATP binding	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables ATP binding	ISO Inferred from Sequence Orthology more info
enables ATP hydrolysis activity	IBA Inferred from Biological aspect of Ancestor more info
enables ATP hydrolysis activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP hydrolysis activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables ATP-dependent protein binding	IDA Inferred from Direct Assay more info	PubMed
enables GTPase activator activity	IDA Inferred from Direct Assay more info	PubMed
enables GTPase activator activity	IEA Inferred from Electronic Annotation more info
enables GTPase activator activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables GTPase activator activity	ISO Inferred from Sequence Orthology more info
enables Roundabout binding	IEA Inferred from Electronic Annotation more info
enables Roundabout binding	ISO Inferred from Sequence Orthology more info
enables actin binding	IDA Inferred from Direct Assay more info	PubMed
enables actin binding	ISO Inferred from Sequence Orthology more info
enables actin filament binding	IBA Inferred from Biological aspect of Ancestor more info
enables actin filament binding	IDA Inferred from Direct Assay more info	PubMed
enables actin filament binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables calmodulin binding	IEA Inferred from Electronic Annotation more info
enables calmodulin binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables microfilament motor activity	IBA Inferred from Biological aspect of Ancestor more info
enables microfilament motor activity	IDA Inferred from Direct Assay more info	PubMed
enables microfilament motor activity	IEA Inferred from Electronic Annotation more info
enables microfilament motor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables microfilament motor activity	ISO Inferred from Sequence Orthology more info
enables microfilament motor activity	ISS Inferred from Sequence or Structural Similarity more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
NOT enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables small GTPase binding	IEA Inferred from Electronic Annotation more info
enables small GTPase binding	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
NOT involved_in ARF protein signal transduction	ISO Inferred from Sequence Orthology more info
involved_in Rho protein signal transduction	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within Rho protein signal transduction	ISO Inferred from Sequence Orthology more info
involved_in Roundabout signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in Roundabout signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in Roundabout signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in actin filament-based movement	IBA Inferred from Biological aspect of Ancestor more info
involved_in actin filament-based movement	IEA Inferred from Electronic Annotation more info
involved_in actin filament-based movement	ISO Inferred from Sequence Orthology more info
involved_in actin filament-based movement	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within establishment of cell polarity	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	IEA Inferred from Electronic Annotation more info
involved_in lamellipodium morphogenesis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within lamellipodium morphogenesis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within macrophage chemotaxis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within monocyte chemotaxis	ISO Inferred from Sequence Orthology more info
involved_in regulation of Rho protein signal transduction	IEA Inferred from Electronic Annotation more info
involved_in regulation of Rho protein signal transduction	ISO Inferred from Sequence Orthology more info
involved_in regulation of Rho protein signal transduction	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
is_active_in actin filament	IBA Inferred from Biological aspect of Ancestor more info
located_in actin filament	IDA Inferred from Direct Assay more info	PubMed
located_in actin filament	IEA Inferred from Electronic Annotation more info
located_in actin filament	ISO Inferred from Sequence Orthology more info
located_in cell cortex	IEA Inferred from Electronic Annotation more info
located_in cell cortex	ISO Inferred from Sequence Orthology more info
located_in cell cortex	ISS Inferred from Sequence or Structural Similarity more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in filopodium tip	ISO Inferred from Sequence Orthology more info
is_active_in lamellipodium	IBA Inferred from Biological aspect of Ancestor more info
located_in lamellipodium	IDA Inferred from Direct Assay more info	PubMed
located_in lamellipodium	ISO Inferred from Sequence Orthology more info
part_of myosin complex	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in ruffle	IBA Inferred from Biological aspect of Ancestor more info
located_in ruffle	IDA Inferred from Direct Assay more info	PubMed
located_in ruffle	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: unconventional myosin-IXb

Names: Unconventional myosin from rat 3; myr 5; unconventional myosin-9b

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001271066.3 → NP_001257995.1 unconventional myosin-IXb isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

Source sequence(s)

JAXUCZ010000016

UniProtKB/TrEMBL

Q4W1H4

Related

ENSRNOP00000073856.2, ENSRNOT00000081321.2

Conserved Domains (6) summary

smart00015
Location:1036 → 1058

IQ; Calmodulin-binding motif

cd04407
Location:1693 → 1878

RhoGAP_myosin_IXB; RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in myosins IXB. Class IX myosins contain a characteristic head domain, a neck domain and a tail domain which contains a C6H2-zinc binding motif and a Rho-GAP domain. Class ...

cd01385
Location:160 → 977

MYSc_Myo9; class IX myosin, motor domain

cd20884
Location:1623 → 1680

C1_Myosin-IXb; protein kinase C conserved region 1 (C1 domain) found in unconventional myosin-IXb and similar proteins

cl21589
Location:988 → 1303

Relaxase; Relaxase/Mobilisation nuclease domain

cl28922
Location:16 → 112

Ubl1_cv_Nsp3_N-like; first ubiquitin-like (Ubl) domain located at the N-terminus of coronavirus SARS-CoV non-structural protein 3 (Nsp3) and related proteins
NM_001271067.3 → NP_001257996.1 unconventional myosin-IXb isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but lacks an alternate internal segment compared to isoform 1.

Source sequence(s)

JAXUCZ010000016

UniProtKB/TrEMBL

Q4W1H3

Conserved Domains (6) summary

smart00015
Location:1031 → 1053

IQ; Calmodulin-binding motif

cd04407
Location:1688 → 1873

RhoGAP_myosin_IXB; RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in myosins IXB. Class IX myosins contain a characteristic head domain, a neck domain and a tail domain which contains a C6H2-zinc binding motif and a Rho-GAP domain. Class ...

cd01385
Location:160 → 972

MYSc_Myo9; class IX myosin, motor domain

cd20884
Location:1618 → 1675

C1_Myosin-IXb; protein kinase C conserved region 1 (C1 domain) found in unconventional myosin-IXb and similar proteins

cl21589
Location:983 → 1298

Relaxase; Relaxase/Mobilisation nuclease domain

cl28922
Location:16 → 112

Ubl1_cv_Nsp3_N-like; first ubiquitin-like (Ubl) domain located at the N-terminus of coronavirus SARS-CoV non-structural protein 3 (Nsp3) and related proteins
NM_012984.5 → NP_037116.2 unconventional myosin-IXb isoform 3

Status: VALIDATED

Description

Transcript Variant: This variant (3) lacks two in-frame exons compared to variant 1. The resulting isoform (3) has the same N- and C-termini but lacks two alternate internal segments compared to isoform 1.

Source sequence(s)

JAXUCZ010000016

UniProtKB/Swiss-Prot

Q63358

Conserved Domains (6) summary

smart00015
Location:1001 → 1023

IQ; Calmodulin-binding motif

cd04407
Location:1658 → 1843

RhoGAP_myosin_IXB; RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in myosins IXB. Class IX myosins contain a characteristic head domain, a neck domain and a tail domain which contains a C6H2-zinc binding motif and a Rho-GAP domain. Class ...

cd01385
Location:160 → 942

MYSc_Myo9; class IX myosin, motor domain

cd20884
Location:1588 → 1645

C1_Myosin-IXb; protein kinase C conserved region 1 (C1 domain) found in unconventional myosin-IXb and similar proteins

cl21589
Location:953 → 1268

Relaxase; Relaxase/Mobilisation nuclease domain

cl28922
Location:16 → 112

Ubl1_cv_Nsp3_N-like; first ubiquitin-like (Ubl) domain located at the N-terminus of coronavirus SARS-CoV non-structural protein 3 (Nsp3) and related proteins