Neurod2 neurogenic differentiation 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Neurod2provided by MGI
Official Full Name: neurogenic differentiation 2provided by MGI
Primary source: MGI:MGI:107755
See related: Ensembl:ENSMUSG00000038255 AllianceGenome:MGI:107755
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Ndrf; bHLHa1
Summary: Enables DNA-binding transcription activator activity, RNA polymerase II-specific; E-box binding activity; and protein heterodimerization activity. Involved in several processes, including cellular response to electrical stimulus; regulation of synapse maturation; and regulation of transcription, DNA-templated. Acts upstream of or within associative learning; behavioral fear response; and neuron development. Located in nucleus. Is expressed in several structures, including genitourinary system; limb; and nervous system. Human ortholog(s) of this gene implicated in developmental and epileptic encephalopathy 72. Orthologous to human NEUROD2 (neuronal differentiation 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in CNS E14 (RPKM 26.6), whole brain E14.5 (RPKM 24.4) and 4 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 D; 11 61.75 cM

Exon count:: 2

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (98216241..98220471, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (98325415..98329645, complement)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Disruption of NEUROD2 causes a neurodevelopmental syndrome with autistic features via cell-autonomous defects in forebrain glutamatergic neurons.
Title: Disruption of NEUROD2 causes a neurodevelopmental syndrome with autistic features via cell-autonomous defects in forebrain glutamatergic neurons.
Terminal neuron localization to the upper cortical plate is controlled by the transcription factor NEUROD2.
Title: Terminal neuron localization to the upper cortical plate is controlled by the transcription factor NEUROD2.
NeuroD2 controls inhibitory circuit formation in the molecular layer of the cerebellum.
Title: NeuroD2 controls inhibitory circuit formation in the molecular layer of the cerebellum.
MiR-219-5p was significantly downregulated after spinal cord injury (SCI) in mouse model. The mRNA and protein levels of NEUROD2 in the SCI group were both remarkably increased in a time-dependent manner. TargetScan predicted that NEUROD2 was the target gene of miRNA-219-5p. Western blot results indicated that miR-219-5p could regulate NEUROD2, eventually promoting the recovery of SCI.
Title: MiR-219-5p promotes spinal cord injury recovery by inhibiting NEUROD2-regulated inflammation and oxidative stress.
the reorganization of DNA methylation in developing neurons involves NEUROD2 and TET2-mediated DNA demethylation.
Title: Reprogramming of DNA methylation at NEUROD2-bound sequences during cortical neuron differentiation.
NeuroD acts in a permissive way to enhance MR-mediated transcription.
Title: Mechanistic Insights in NeuroD Potentiation of Mineralocorticoid Receptor Signaling.
Using genome-wide, mRNA-based microarray analysis, study found that NeuroD2 is actually one of hundreds of genes whose mRNA levels are suppressed by synaptic activity, in a manner dependent upon N-methyl d-aspartate receptor (NMDAR) activation. Study confirmed this observation both in vitro and in vivo and provide evidence that this happens at the level of transcription and not mRNA stability.
Title: Synaptic activity suppresses expression of neurogenic differentiation factor 2 in an NMDA receptor-dependent manner.
Genome-wide target gene analysis of the neurogenic transcription factor NEUROD2 has uncovered a novel gene expression mechanism by which differentiating cortical excitatory neurons fine-tune the extent of store-operated calcium entry. In vivo analysis identified Stim1 (stromal interaction molecule 1) as a primary target of NEUROD2.
Title: NEUROD2 Regulates Stim1 Expression and Store-Operated Calcium Entry in Cortical Neurons.
Our results reveal an important function for NeuroD2 in balancing synaptic neurotransmission and intrinsic excitability and offer insight into how these processes are coordinated during cortical development.
Title: The transcription factor NeuroD2 coordinates synaptic innervation and cell intrinsic properties to control excitability of cortical pyramidal neurons.
Our analysis of in vivo NEUROD2 target genes offers mechanistic insight into signaling pathways that regulate neuronal migration and axon guidance and identifies genes that are likely to be required for proper cortical development.
Title: Genome-wide target analysis of NEUROD2 provides new insights into regulation of cortical projection neuron migration and differentiation.

Submit: New GeneRIF Correction See all GeneRIFs (20)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (4) 1 citation
Endonuclease-mediated (2)

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables E-box binding	IBA Inferred from Biological aspect of Ancestor more info
enables E-box binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein dimerization activity	IEA Inferred from Electronic Annotation more info
enables protein heterodimerization activity	IDA Inferred from Direct Assay more info	PubMed
enables sequence-specific double-stranded DNA binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within associative learning	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in axon development	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within behavioral fear response	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in cellular response to calcium ion	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to electrical stimulus	IDA Inferred from Direct Assay more info	PubMed
involved_in cerebellar cortex development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within multicellular organism development	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of synapse maturation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within nervous system development	IEA Inferred from Electronic Annotation more info
involved_in nervous system development	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within neuron development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of DNA-binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of calcium-mediated signaling	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of neuron differentiation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of synapse maturation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of synaptic plasticity	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in protein ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in sensory organ development	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: neurogenic differentiation factor 2

Names: neuroD-related factor

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_010895.3 → NP_035025.3 neurogenic differentiation factor 2

See identical proteins and their annotated locations for NP_035025.3

Status: VALIDATED

Source sequence(s)

AK158767, CD741852, CJ063668, CJ237767, CJ237963

Consensus CDS

CCDS25343.1

UniProtKB/Swiss-Prot

Q61952, Q62414, Q925V5

UniProtKB/TrEMBL

Q3TYB2

Related

ENSMUSP00000041373.7, ENSMUST00000041685.7

Conserved Domains (2) summary

cd19720
Location:89 → 181

bHLH_TS_NeuroD2; basic helix-loop-helix (bHLH) domain found in neurogenic differentiation factor 2 (NeuroD2) and similar proteins

pfam12533
Location:181 → 311

Neuro_bHLH; Neuronal helix-loop-helix transcription factor

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000077.7 Reference GRCm39 C57BL/6J

Range

98216241..98220471 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030245643.2 → XP_030101503.1 neurogenic differentiation factor 2 isoform X1

UniProtKB/Swiss-Prot

Q61952, Q62414, Q925V5

UniProtKB/TrEMBL

Q3TYB2

Conserved Domains (2) summary

cd19720
Location:89 → 181

bHLH_TS_NeuroD2; basic helix-loop-helix (bHLH) domain found in neurogenic differentiation factor 2 (NeuroD2) and similar proteins

pfam12533
Location:181 → 311

Neuro_bHLH; Neuronal helix-loop-helix transcription factor