Mapre3 microtubule-associated protein, RP/EB family, member 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Mapre3provided by MGI
Official Full Name: microtubule-associated protein, RP/EB family, member 3provided by MGI
Primary source: MGI:MGI:2140967
See related: Ensembl:ENSMUSG00000029166 AllianceGenome:MGI:2140967
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: EB2; EB3; RP3; EBF3
Summary: Enables microtubule plus-end binding activity. Involved in positive regulation of cyclin-dependent protein serine/threonine kinase activity and positive regulation of transcription, DNA-templated. Acts upstream of or within positive regulation of microtubule plus-end binding activity. Located in cytoplasm; microtubule plus-end; and midbody. Colocalizes with microtubule. Orthologous to human MAPRE3 (microtubule associated protein RP/EB family member 3). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in testis adult (RPKM 61.2), frontal lobe adult (RPKM 48.7) and 24 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 B1; 5 16.9 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (30972075..31023447)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (30814606..30866106)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Cytoskeleton Protein EB3 Contributes to Dendritic Spines Enlargement and Enhances Their Resilience to Toxic Effects of Beta-Amyloid.
Title: Cytoskeleton Protein EB3 Contributes to Dendritic Spines Enlargement and Enhances Their Resilience to Toxic Effects of Beta-Amyloid.
The authors propose that EB3 is involved in regulation of dendritic spines morphology, in part due to its association with STIM2, and that modulation of EB3 expression is a potential way to overcome synaptic loss during Alzheimer's disease.
Title: Stim2-Eb3 Association and Morphology of Dendritic Spines in Hippocampal Neurons.
EB3 binds to IP3 receptors, promotes IP3R clustering and Ca2+ signals in endothelial cells.
Title: Microtubule-Associated Protein EB3 Regulates IP3 Receptor Clustering and Ca(2+) Signaling in Endothelial Cells.
We describe here a novel function for tau as a direct regulator of End binding (EB) proteins in differentiating neuronal cells
Title: Tau regulates the localization and function of End-binding proteins 1 and 3 in developing neuronal cells.
MAP1B interacts directly with EB1 and EB3 (EBs), two core 'microtubule plus-end tracking proteins' ( TIPs), and sequesters them in the cytosol of developing neuronal cells.
Title: MAP1B regulates microtubule dynamics by sequestering EB1/3 in the cytosol of developing neuronal cells.
DDA3 is a novel microtubule-associated protein that binds to EB3, and results implicate DDA3 and EB3 in the beta-catenin-mediated growth signaling.
Title: p53 downstream target DDA3 is a novel microtubule-associated protein that interacts with end-binding protein EB3 and activates beta-catenin pathway.
Two +TIPs, CLIP-170 and end-binding protein 3 (EB3), turn over rapidly on MT ends. Diffusion of CLIP-170 and EB3 appears to be rate limiting for their binding to MT plus ends.
Title: Dynamic behavior of GFP-CLIP-170 reveals fast protein turnover on microtubule plus ends.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH102194 (e-PCR)
- UniSTS:96528

AI790651 (e-PCR)
- UniSTS:165392

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables microtubule binding	IDA Inferred from Direct Assay more info	PubMed
enables microtubule plus-end binding	IBA Inferred from Biological aspect of Ancestor more info
enables microtubule plus-end binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within cell cycle	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cell division	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cyclin-dependent protein serine/threonine kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of microtubule plus-end binding	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of protein kinase activity	ISO Inferred from Sequence Orthology more info
involved_in protein localization to microtubule	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of microtubule polymerization	ISO Inferred from Sequence Orthology more info
involved_in regulation of microtubule polymerization or depolymerization	IBA Inferred from Biological aspect of Ancestor more info
involved_in spindle assembly	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasmic microtubule	IBA Inferred from Biological aspect of Ancestor more info
colocalizes_with cytoplasmic microtubule	ISO Inferred from Sequence Orthology more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
colocalizes_with microtubule	IDA Inferred from Direct Assay more info	PubMed
located_in microtubule cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
located_in microtubule cytoskeleton	ISO Inferred from Sequence Orthology more info
is_active_in microtubule organizing center	IBA Inferred from Biological aspect of Ancestor more info
is_active_in microtubule plus-end	IBA Inferred from Biological aspect of Ancestor more info
located_in microtubule plus-end	IDA Inferred from Direct Assay more info	PubMed
located_in midbody	IDA Inferred from Direct Assay more info	PubMed
located_in mitotic spindle astral microtubule end	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in spindle midzone	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: microtubule-associated protein RP/EB family member 3

Names: EB1 protein family member 3; end-binding protein 3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001310516.2 → NP_001297445.1 microtubule-associated protein RP/EB family member 3 isoform 2

See identical proteins and their annotated locations for NP_001297445.1

Status: VALIDATED

Source sequence(s)

AC109606, AC109611

Consensus CDS

CCDS80240.1

UniProtKB/TrEMBL

D3Z6G3

Conserved Domains (1) summary

COG5217
Location:16 → 264

BIM1; Microtubule-binding protein involved in cell cycle control [Cell division and chromosome partitioning / Cytoskeleton]
NM_001425743.1 → NP_001412672.1 microtubule-associated protein RP/EB family member 3 isoform 1

Status: VALIDATED

Source sequence(s)

AC109606, AC109611

UniProtKB/Swiss-Prot

Q61167, Q6PER3

UniProtKB/TrEMBL

Q2UZW7
NM_001425744.1 → NP_001412673.1 microtubule-associated protein RP/EB family member 3 isoform 2

Status: VALIDATED

Source sequence(s)

AC109606, AC109611

UniProtKB/TrEMBL

D3Z6G3
NM_133350.3 → NP_579928.1 microtubule-associated protein RP/EB family member 3 isoform 1

See identical proteins and their annotated locations for NP_579928.1

Status: VALIDATED

Source sequence(s)

AC109606, AC109611

Consensus CDS

CCDS19163.1

UniProtKB/Swiss-Prot

Q61167, Q6PER3

UniProtKB/TrEMBL

Q2UZW7

Related

ENSMUSP00000031058.9, ENSMUST00000031058.15

Conserved Domains (1) summary

COG5217
Location:16 → 279

BIM1; Microtubule-binding protein involved in cell cycle control [Cell division and chromosome partitioning / Cytoskeleton]

RNA

NR_189585.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC109606, AC109611

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000071.7 Reference GRCm39 C57BL/6J

Range

30972075..31023447

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030254024.2 → XP_030109884.1 microtubule-associated protein RP/EB family member 3 isoform X2

UniProtKB/TrEMBL

D3Z6G3

Conserved Domains (1) summary

COG5217
Location:16 → 264

BIM1; Microtubule-binding protein involved in cell cycle control [Cell division and chromosome partitioning / Cytoskeleton]
XM_030254022.2 → XP_030109882.1 microtubule-associated protein RP/EB family member 3 isoform X1

UniProtKB/Swiss-Prot

Q61167, Q6PER3

UniProtKB/TrEMBL

Q2UZW7

Conserved Domains (1) summary

COG5217
Location:16 → 279

BIM1; Microtubule-binding protein involved in cell cycle control [Cell division and chromosome partitioning / Cytoskeleton]