Arhgap32 Rho GTPase activating protein 32 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Arhgap32provided by MGI
Official Full Name: Rho GTPase activating protein 32provided by MGI
Primary source: MGI:MGI:2450166
See related: Ensembl:ENSMUSG00000041444 AllianceGenome:MGI:2450166
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Grit; Rics; Gc-gap; Px-rics; p250Gap; mKIAA0712; p200Rhogap; 3426406O18Rik
Summary: Predicted to enable GTPase activator activity. Predicted to be involved in small GTPase mediated signal transduction. Predicted to act upstream of or within Rho protein signal transduction. Located in actin cytoskeleton; cell cortex; and postsynaptic density. Is expressed in several structures, including brain; genitourinary system; liver; lung; and spleen. Used to study autism spectrum disorder. Orthologous to human ARHGAP32 (Rho GTPase activating protein 32). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in cortex adult (RPKM 9.5), frontal lobe adult (RPKM 9.3) and 22 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 A4; 9 17.48 cM

Exon count:: 27

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (31927921..32176061)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (32016625..32268443)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Our results suggest that PX-RICS-mediated GABAAR trafficking is a key target for GABAergic plasticity and its dysfunction leads to atypical emotional processing underlying autism.
Title: The Autism-Related Protein PX-RICS Mediates GABAergic Synaptic Plasticity in Hippocampal Neurons and Emotional Learning in Mice.
PX-RICS-deficient mice exhibit ASD-like social behaviours and ASD-related comorbidities. PX-RICS-deficient neurons show reduced surface gamma-aminobutyric acid type A receptor (GABAAR) levels and impaired GABAAR-mediated synaptic transmission.
Title: PX-RICS-deficient mice mimic autism spectrum disorder in Jacobsen syndrome through impaired GABAA receptor trafficking.
leptin stimulates the MAPK pathways and CREB dependent transcription. This then leads to an increase in miR132 that suppresses expression of the Rac1 inhibitor p250GAP, thus activating Rac1 and promoting synaptogenesis.
Title: Leptin induces hippocampal synaptogenesis via CREB-regulated microRNA-132 suppression of p250GAP.
Overexpression of p250GAP abolishes brain-derived neurotrophic factor (BDNF)-induced branching.
Title: BDNF promotes axon branching of retinal ganglion cells via miRNA-132 and p250GAP.
Cdh1-APC together with the RhoA regulators p250GAP and Smurf1 controls axon growth in the mammalian brain
Title: p250GAP is a novel player in the Cdh1-APC/Smurf1 pathway of axon growth regulation.
Results suggest that PX-RICS ensures the efficient entry of the N-cadherin/beta-catenin complex into the secretory pathway, and thereby regulates the amount of N-cadherin available for cell adhesion and FGFR4-mediated signaling.
Title: PX-RICS mediates ER-to-Golgi transport of the N-cadherin/beta-catenin complex.
These results suggest that PX-RICS is involved in early brain development including extension of axons and dendrites, and postnatal remodeling and fine-tuning of neural circuits.
Title: PX-RICS, a novel splicing variant of RICS, is a main isoform expressed during neural development.
p250GAP plays an important role in NMDA receptor-mediated regulation of RhoA activity leading to spine morphological plasticity.
Title: Regulation of dendritic spine morphology by an NMDA receptor-associated Rho GTPase-activating protein, p250GAP.
p200 RhoGAP can mediate cross-talks between Ras- and Rho-regulated signaling pathways in cell growth regulation
Title: p200 RhoGAP promotes cell proliferation by mediating cross-talk between Ras and Rho signaling pathways.
RICS plays an important role in neurite extension
Title: Role of the Rho GTPase-activating protein RICS in neurite outgrowth.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (1) 1 citation
Endonuclease-mediated (3)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

40.MMHAP25FLF4.seq (e-PCR)
- UniSTS:124360

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables GTPase activator activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activator activity	ISO Inferred from Sequence Orthology more info	PubMed
enables phosphatidylinositol binding	IEA Inferred from Electronic Annotation more info
enables phosphatidylinositol phosphate binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within Rho protein signal transduction	ISO Inferred from Sequence Orthology more info	PubMed
involved_in positive regulation of axon extension	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of neuron migration	ISO Inferred from Sequence Orthology more info
involved_in signal transduction	IEA Inferred from Electronic Annotation more info
involved_in small GTPase-mediated signal transduction	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
is_active_in Golgi apparatus	IBA Inferred from Biological aspect of Ancestor more info
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
is_active_in actin cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in actin cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
is_active_in cell cortex	IBA Inferred from Biological aspect of Ancestor more info
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
located_in cell projection	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endosome	IEA Inferred from Electronic Annotation more info
is_active_in fibrillar center	IBA Inferred from Biological aspect of Ancestor more info
located_in fibrillar center	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in nucleoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in postsynaptic density	IDA Inferred from Direct Assay more info	PubMed
located_in synapse	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: rho GTPase-activating protein 32

Names: GAB-associated Cdc42/Rac GTPase-activating protein; GTPase-activating protein for Cdc42 and Rac1; Rho GTPase activating protein 33 pseudogene; brain-specific Rho GTPase-activating protein; rac GTPase activating protein; rho-type GTPase-activating protein 32; rho/Cdc42/Rac GTPase-activating protein RICS; rhoGAP involved in the beta-catenin-N-cadherin and NMDA receptor signaling

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001195632.2 → NP_001182561.1 rho GTPase-activating protein 32 isoform 1

See identical proteins and their annotated locations for NP_001182561.1

Status: VALIDATED

Source sequence(s)

AC132114, AC134607

Consensus CDS

CCDS57666.1

UniProtKB/Swiss-Prot

B9EHJ8, Q6A010, Q811P8

UniProtKB/TrEMBL

I7HFI3

Related

ENSMUSP00000133898.2, ENSMUST00000174641.8

Conserved Domains (3) summary

cd07298
Location:127 → 241

PX_RICS; The phosphoinositide binding Phox Homology domain of PX-RICS

cd11835
Location:263 → 316

SH3_ARHGAP32_33; Src homology 3 domain of Rho GTPase-activating proteins 32 and 33, and similar proteins

cd04384
Location:368 → 562

RhoGAP_CdGAP; RhoGAP_CdGAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of CdGAP-like proteins; CdGAP contains an N-terminal RhoGAP domain and a C-terminal proline-rich region, and it is active on both Cdc42 and Rac1 but not RhoA. CdGAP ...
NM_001370957.1 → NP_001357886.1 rho GTPase-activating protein 32 isoform 4

Status: VALIDATED

Source sequence(s)

AC132114, AC134607, AC160635

UniProtKB/TrEMBL

I7HFI3

Conserved Domains (4) summary

cd07298
Location:87 → 201

PX_RICS; The phosphoinositide binding Phox Homology domain of PX-RICS

cd11835
Location:223 → 276

SH3_ARHGAP32_33; Src homology 3 domain of Rho GTPase-activating proteins 32 and 33, and similar proteins

PHA03247
Location:1197 → 1579

PHA03247; large tegument protein UL36; Provisional

cd04384
Location:328 → 522

RhoGAP_CdGAP; RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of CdGAP-like proteins; CdGAP contains an N-terminal RhoGAP domain and a C-terminal proline-rich region, and it is active on both Cdc42 and Rac1 but not RhoA. CdGAP is recruited to ...
NM_001370958.1 → NP_001357887.1 rho GTPase-activating protein 32 isoform 3

Status: VALIDATED

Source sequence(s)

AC132114, AC134607, AC160635

UniProtKB/TrEMBL

I7HFI3

Conserved Domains (4) summary

cd07298
Location:101 → 215

PX_RICS; The phosphoinositide binding Phox Homology domain of PX-RICS

cd11835
Location:237 → 290

SH3_ARHGAP32_33; Src homology 3 domain of Rho GTPase-activating proteins 32 and 33, and similar proteins

PHA03247
Location:1211 → 1593

PHA03247; large tegument protein UL36; Provisional

cd04384
Location:342 → 536

RhoGAP_CdGAP; RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of CdGAP-like proteins; CdGAP contains an N-terminal RhoGAP domain and a C-terminal proline-rich region, and it is active on both Cdc42 and Rac1 but not RhoA. CdGAP is recruited to ...
NM_177379.5 → NP_796353.3 rho GTPase-activating protein 32 isoform 2

See identical proteins and their annotated locations for NP_796353.3

Status: VALIDATED

Source sequence(s)

AC132114, AC134607

Consensus CDS

CCDS22951.3

UniProtKB/TrEMBL

I7HFI3

Related

ENSMUSP00000128448.3, ENSMUST00000168954.9

Conserved Domains (1) summary

cd04384
Location:19 → 213

RhoGAP_CdGAP; RhoGAP_CdGAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of CdGAP-like proteins; CdGAP contains an N-terminal RhoGAP domain and a C-terminal proline-rich region, and it is active on both Cdc42 and Rac1 but not RhoA. CdGAP ...

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000075.7 Reference GRCm39 C57BL/6J

Range

31927921..32176061

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006510445.5 → XP_006510508.1 rho GTPase-activating protein 32 isoform X3

See identical proteins and their annotated locations for XP_006510508.1

UniProtKB/TrEMBL

I7HFI3

Conserved Domains (4) summary

cd07298
Location:101 → 215

PX_RICS; The phosphoinositide binding Phox Homology domain of PX-RICS

cd11835
Location:237 → 290

SH3_ARHGAP32_33; Src homology 3 domain of Rho GTPase-activating proteins 32 and 33, and similar proteins

PHA03247
Location:1211 → 1593

PHA03247; large tegument protein UL36; Provisional

cd04384
Location:342 → 536

RhoGAP_CdGAP; RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of CdGAP-like proteins; CdGAP contains an N-terminal RhoGAP domain and a C-terminal proline-rich region, and it is active on both Cdc42 and Rac1 but not RhoA. CdGAP is recruited to ...
XM_030244425.2 → XP_030100285.1 rho GTPase-activating protein 32 isoform X4

UniProtKB/TrEMBL

I7HFI3

Conserved Domains (4) summary

cd07298
Location:87 → 201

PX_RICS; The phosphoinositide binding Phox Homology domain of PX-RICS

cd11835
Location:223 → 276

SH3_ARHGAP32_33; Src homology 3 domain of Rho GTPase-activating proteins 32 and 33, and similar proteins

PHA03247
Location:1197 → 1579

PHA03247; large tegument protein UL36; Provisional

cd04384
Location:328 → 522

RhoGAP_CdGAP; RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of CdGAP-like proteins; CdGAP contains an N-terminal RhoGAP domain and a C-terminal proline-rich region, and it is active on both Cdc42 and Rac1 but not RhoA. CdGAP is recruited to ...
XM_036155080.1 → XP_036010973.1 rho GTPase-activating protein 32 isoform X5

UniProtKB/TrEMBL

I7HFI3

Conserved Domains (2) summary

PHA03247
Location:912 → 1294

PHA03247; large tegument protein UL36; Provisional

cd04384
Location:43 → 237

RhoGAP_CdGAP; RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of CdGAP-like proteins; CdGAP contains an N-terminal RhoGAP domain and a C-terminal proline-rich region, and it is active on both Cdc42 and Rac1 but not RhoA. CdGAP is recruited to ...
XM_030244426.2 → XP_030100286.1 rho GTPase-activating protein 32 isoform X5

UniProtKB/TrEMBL

I7HFI3

Conserved Domains (2) summary

PHA03247
Location:912 → 1294

PHA03247; large tegument protein UL36; Provisional

cd04384
Location:43 → 237

RhoGAP_CdGAP; RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of CdGAP-like proteins; CdGAP contains an N-terminal RhoGAP domain and a C-terminal proline-rich region, and it is active on both Cdc42 and Rac1 but not RhoA. CdGAP is recruited to ...
XM_036155079.1 → XP_036010972.1 rho GTPase-activating protein 32 isoform X2

UniProtKB/TrEMBL

I7HFI3

Conserved Domains (4) summary

cd07298
Location:129 → 243

PX_RICS; The phosphoinositide binding Phox Homology domain of PX-RICS

cd11835
Location:265 → 318

SH3_ARHGAP32_33; Src homology 3 domain of Rho GTPase-activating proteins 32 and 33, and similar proteins

PHA03247
Location:1239 → 1621

PHA03247; large tegument protein UL36; Provisional

cd04384
Location:370 → 564

RhoGAP_CdGAP; RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of CdGAP-like proteins; CdGAP contains an N-terminal RhoGAP domain and a C-terminal proline-rich region, and it is active on both Cdc42 and Rac1 but not RhoA. CdGAP is recruited to ...
XM_006510442.4 → XP_006510505.1 rho GTPase-activating protein 32 isoform X1

UniProtKB/TrEMBL

I7HFI3

Conserved Domains (4) summary

cd07298
Location:141 → 255

PX_RICS; The phosphoinositide binding Phox Homology domain of PX-RICS

cd11835
Location:277 → 330

SH3_ARHGAP32_33; Src homology 3 domain of Rho GTPase-activating proteins 32 and 33, and similar proteins

PHA03247
Location:1251 → 1633

PHA03247; large tegument protein UL36; Provisional

cd04384
Location:382 → 576

RhoGAP_CdGAP; RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of CdGAP-like proteins; CdGAP contains an N-terminal RhoGAP domain and a C-terminal proline-rich region, and it is active on both Cdc42 and Rac1 but not RhoA. CdGAP is recruited to ...

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

NG_020962.1: Suppressed sequence

Description

NG_020962.1: This RefSeq was permanently suppressed because it is now thought that this gene does encode a protein.