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    Prkra protein kinase, interferon inducible double stranded RNA dependent activator [ Mus musculus (house mouse) ]

    Gene ID: 23992, updated on 21-Apr-2024

    Summary

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    Official Symbol
    Prkraprovided by MGI
    Official Full Name
    protein kinase, interferon inducible double stranded RNA dependent activatorprovided by MGI
    Primary source
    MGI:MGI:1344375
    See related
    Ensembl:ENSMUSG00000002731 AllianceGenome:MGI:1344375
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    PRK; RAX; Pact; lear
    Summary
    Predicted to enable RNA binding activity; protein homodimerization activity; and protein kinase binding activity. Acts upstream of or within several processes, including cellular response to oxidative stress; ear morphogenesis; and positive regulation of intrinsic apoptotic signaling pathway. Predicted to be located in cytosol and nucleoplasm. Predicted to be part of RISC-loading complex. Is expressed in several structures, including 4-cell stage embryo; central nervous system; olfactory epithelium; and retina. Human ortholog(s) of this gene implicated in dystonia 16. Orthologous to human PRKRA (protein activator of interferon induced protein kinase EIF2AK2). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Ubiquitous expression in CNS E18 (RPKM 16.2), CNS E14 (RPKM 14.3) and 28 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    2 C3; 2 45.07 cM
    Exon count:
    11
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 2 NC_000068.8 (76436385..76478614, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 2 NC_000068.7 (76609215..76648220, complement)

    Chromosome 2 - NC_000068.8Genomic Context describing neighboring genes Neighboring gene RNA binding motif protein 45 Neighboring gene oxysterol binding protein-like 6 Neighboring gene STARR-seq mESC enhancer starr_04893 Neighboring gene STARR-positive B cell enhancer ABC_E2624 Neighboring gene pejvakin Neighboring gene FK506 binding protein 7

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Prkra Mutation Alters mRNA Expression During Embryonic External Ear Development. Liu W, et al. J Craniofac Surg, 2023 Jun 1. PMID 37185168
    2. Prkra Mutation Alters Long Noncoding RNA Expression During Embryonic External Ear Development. Liu W, et al. J Craniofac Surg, 2023 Mar-Apr 01. PMID 35968982
    3. Point Mutation in Prkra Alters miRNA Expression During Embryonic External Ear Development. Liu W, et al. J Craniofac Surg, 2023 Mar-Apr 01. PMID 35968958
    4. PKR deficiency alters E. coli-induced sickness behaviors but does not exacerbate neuroimmune responses or bacterial load. Poon DC, et al. J Neuroinflammation, 2015 Nov 19. PMID 26585788, Free PMC Article
    5. PACT/RAX regulates the migration of cerebellar granule neurons in the developing cerebellum. Yong Y, et al. Sci Rep, 2015 Jan 22. PMID 25609658, Free PMC Article

    See all (55) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Cell-type-specific need of Ddx3 and PACT for interferon induction by RNA viruses.
      Title: Cell-type-specific need of Ddx3 and PACT for interferon induction by RNA viruses.
    2. Prkra Mutation Alters mRNA Expression During Embryonic External Ear Development.
      Title: Prkra Mutation Alters mRNA Expression During Embryonic External Ear Development.
    3. Point Mutation in Prkra Alters miRNA Expression During Embryonic External Ear Development.
      Title: Point Mutation in Prkra Alters miRNA Expression During Embryonic External Ear Development.
    4. Prkra Mutation Alters Long Noncoding RNA Expression During Embryonic External Ear Development.
      Title: Prkra Mutation Alters Long Noncoding RNA Expression During Embryonic External Ear Development.
    5. PACT establishes a posttranscriptional brake on mitochondrial biogenesis by promoting the maturation of miR-181c.
      Title: PACT establishes a posttranscriptional brake on mitochondrial biogenesis by promoting the maturation of miR-181c.
    6. Dicer represses the interferon response and the double-stranded RNA-activated protein kinase pathway in mouse embryonic stem cells.
      Title: Dicer represses the interferon response and the double-stranded RNA-activated protein kinase pathway in mouse embryonic stem cells.
    7. Opposite actions of two dsRNA-binding proteins PACT and TRBP on RIG-I mediated signaling.
      Title: Opposite actions of two dsRNA-binding proteins PACT and TRBP on RIG-I mediated signaling.
    8. A genome-scale CRISPR screen reveals factors regulating Wnt-dependent renewal of mouse gastric epithelial cells.
      Title: A genome-scale CRISPR screen reveals factors regulating Wnt-dependent renewal of mouse gastric epithelial cells.
    9. PACT enhances mammalian embryonic neural stem cell properties as assessed in vitro and in vivo. PACT augments the Notch signaling pathway, a master regulator of neural stem cell fate, at the transcriptional level.
      Title: PACT increases mammalian embryonic neural stem cell properties by facilitating activation of the notch signaling pathway.
    10. TARBP2, but not PRKRA, functions in miRNA biogenesis of a subclass of miRNAs, and suggesting that functional redundancy between TARBP2 and PRKRA does not involve miRNA biogenesis.
      Title: Functional Redundancy of DICER Cofactors TARBP2 and PRKRA During Murine Embryogenesis Does Not Involve miRNA Biogenesis.
    Submit: New GeneRIF Correction See all GeneRIFs (23)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables double-stranded RNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables double-stranded RNA binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables enzyme binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables identical protein binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables pre-miRNA binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein homodimerization activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables protein kinase activator activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables protein kinase binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables siRNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Process Evidence Code Pubs
    involved_in RISC complex assembly ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in antiviral innate immune response ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within cellular response to oxidative stress IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within ear development IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in miRNA processing ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within middle ear morphogenesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within outer ear morphogenesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of cell population proliferation ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within positive regulation of intrinsic apoptotic signaling pathway IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in pre-miRNA processing ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within protein phosphorylation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein stabilization ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in regulation of regulatory ncRNA processing IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within regulatory ncRNA-mediated gene silencing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in siRNA processing IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in siRNA processing ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within skeletal system morphogenesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of RISC complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of RISC-loading complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of RISC-loading complex ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in cytoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in intracellular anatomical structure IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    interferon-inducible double-stranded RNA-dependent protein kinase activator A
    Names
    PKR-associated protein X
    PKR-associating protein X
    protein activator of the interferon-induced protein kinase

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001421199.1 → NP_001408128.1  interferon-inducible double-stranded RNA-dependent protein kinase activator A isoform 2

      Status: VALIDATED

      Source sequence(s)
      AL928867

    2. NM_001421200.1 → NP_001408129.1  interferon-inducible double-stranded RNA-dependent protein kinase activator A isoform 3

      Status: VALIDATED

      Source sequence(s)
      AL928867

    3. NM_001421201.1 → NP_001408130.1  interferon-inducible double-stranded RNA-dependent protein kinase activator A isoform 4

      Status: VALIDATED

      Source sequence(s)
      AL928867

    4. NM_001421202.1 → NP_001408131.1  interferon-inducible double-stranded RNA-dependent protein kinase activator A isoform 5

      Status: VALIDATED

      Source sequence(s)
      AL928867

    5. NM_001421203.1 → NP_001408132.1  interferon-inducible double-stranded RNA-dependent protein kinase activator A isoform 6

      Status: VALIDATED

      Source sequence(s)
      AL928867

    6. NM_001421204.1 → NP_001408133.1  interferon-inducible double-stranded RNA-dependent protein kinase activator A isoform 7

      Status: VALIDATED

      Source sequence(s)
      AL928867

    7. NM_001421205.1 → NP_001408134.1  interferon-inducible double-stranded RNA-dependent protein kinase activator A isoform 7

      Status: VALIDATED

      Source sequence(s)
      AL928867

    8. NM_001421206.1 → NP_001408135.1  interferon-inducible double-stranded RNA-dependent protein kinase activator A isoform 8

      Status: VALIDATED

      Source sequence(s)
      AL928867

    9. NM_001421207.1 → NP_001408136.1  interferon-inducible double-stranded RNA-dependent protein kinase activator A isoform 9

      Status: VALIDATED

      Source sequence(s)
      AL928867

    10. NM_011871.4 → NP_036001.1  interferon-inducible double-stranded RNA-dependent protein kinase activator A isoform 1

      See identical proteins and their annotated locations for NP_036001.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) encodes the longest isoform (1).
      Source sequence(s)
      AL928867
      Consensus CDS
      CCDS16159.1
      UniProtKB/Swiss-Prot
      Q9CZB7, Q9WTX2
      Related
      ENSMUSP00000002808.7, ENSMUST00000002808.7
      Conserved Domains (3) summary
      cd19889
      Location:32 → 102
      DSRM_PRKRA_rpt1; first double-stranded RNA binding motif of protein activator of the interferon-induced protein kinase (PRKRA) and similar proteins
      cd19891
      Location:126 → 192
      DSRM_PRKRA_rpt2; second double-stranded RNA binding motif of protein activator of the interferon-induced protein kinase (PRKRA) and similar proteins
      cd19892
      Location:239 → 310
      DSRM_PRKRA_rpt3; third double-stranded RNA binding motif of protein activator of the interferon-induced protein kinase (PRKRA) and similar proteins

    RNA

    1. NR_185269.1 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AL928867

    2. NR_185270.1 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AL928867

    3. NR_185271.1 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AL928867

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000068.8 Reference GRCm39 C57BL/6J

      Range
      76436385..76478614 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_030250935.2 → XP_030106795.1  interferon-inducible double-stranded RNA-dependent protein kinase activator A isoform X2

      Conserved Domains (3) summary
      cd19889
      Location:113 → 183
      DSRM_PRKRA_rpt1; first double-stranded RNA binding motif of protein activator of the interferon-induced protein kinase (PRKRA) and similar proteins
      cd19891
      Location:207 → 273
      DSRM_PRKRA_rpt2; second double-stranded RNA binding motif of protein activator of the interferon-induced protein kinase (PRKRA) and similar proteins
      cl00054
      Location:320 → 342
      DSRM_SF; double-stranded RNA binding motif (DSRM) superfamily
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9WTX2.1 GenPept UniProtKB/Swiss-Prot:Q9WTX2

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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