Tssk2 testis-specific serine kinase 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Tssk2provided by MGI
Official Full Name: testis-specific serine kinase 2provided by MGI
Primary source: MGI:MGI:1347559
See related: Ensembl:ENSMUSG00000045521 AllianceGenome:MGI:1347559
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Tsk2; DGS-G; SPOGA2; Stk22b
Summary: Enables magnesium ion binding activity and protein serine/threonine kinase activity. Involved in peptidyl-serine phosphorylation and spermatid development. Located in acrosomal vesicle and centriole. Is expressed in embryo; seminiferous tubule; spermatid; and testis. Orthologous to human TSSK2 (testis specific serine kinase 2). [provided by Alliance of Genome Resources, Apr 2022]
Orthologs: human all
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Genomic context

Location:: 16 A3; 16 11.09 cM

Exon count:: 1

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	16	NC_000082.7 (17716501..17717888)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	16	NC_000082.6 (17898637..17900024)

Chromosome 16 - NC_000082.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HSP90 function abolished the catalytic activities of TSSK4 and -6 but did not significantly alter the specific activities of TSSK1 and -2. Inhibition of HSP90 resulted in increased TSSK ubiquitination and proteasomal degradation
Title: Heat shock protein 90 functions to stabilize and activate the testis-specific serine/threonine kinases, a family of kinases essential for male fertility.
Tssk1 and Tssk2 play an important role in the regulation of sperm function.
Title: [Cloning and expression of Tssk1 & Tssk2 in mice and the presence & localization of them in mature sperm].
Results reveal TSSK1- and TSSK2-dependent functions of a transformed chromatoid body in post-meiotic cytodifferentiation of spermatids.
Title: Functional transformation of the chromatoid body in mouse spermatids requires testis-specific serine/threonine kinases.
SPAG16L can be phosphorylated by TSSK2 in vitro.
Title: Phosphorylation of mouse sperm axoneme central apparatus protein SPAG16L by a testis-specific kinase, TSSK2.
Deletion of Tssk2 kinase/substrate pair is predicted to play an indispensable role during spermiogenesis.
Title: TSKS concentrates in spermatid centrioles during flagellogenesis.
TSSK1 and TSKS persist in sperm, define a critical role of TSKS' N-terminus in enzyme interaction, identify Ser 281 as a TSKS phosphorylation site and indicate an indispensable role for TSSK 1 and 2 in spermiogenesis.
Title: Targeted deletion of Tssk1 and 2 causes male infertility due to haploinsufficiency.
The expression patterns of Tsk2 is documented throughout spermiogenesis showing a late association of Tsk2 with tail-like structures in the lumen of the seminiferous tubule.
Title: Immunohistochemical analysis of the expression of two serine-threonine kinases in the maturing mouse testis.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (1) 1 citation

General gene information

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Markers

Tssk2 (e-PCR), detects polymorphism
- UniSTS:265751

UniSTS:260486 (e-PCR)
- UniSTS:260486

Dgsh (e-PCR), detects polymorphism
- UniSTS:260486

Dgsh (e-PCR), detects polymorphism
- UniSTS:260487

UniSTS:260495 (e-PCR)
- UniSTS:260495

Tssk2 (e-PCR), detects polymorphism
- UniSTS:260495

Stk22b (e-PCR), detects polymorphism
- UniSTS:260496

BE110023 (e-PCR)
- UniSTS:245164

AF019926 (e-PCR)
- UniSTS:158939

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables magnesium ion binding	IDA Inferred from Direct Assay more info	PubMed
enables magnesium ion binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables protein-containing complex binding	IPI Inferred from Physical Interaction more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within cell differentiation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within multicellular organism development	IEA Inferred from Electronic Annotation more info
involved_in peptidyl-serine phosphorylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in protein autophosphorylation	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in spermatid development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within spermatogenesis	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in acrosomal vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in centriole	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: testis-specific serine/threonine-protein kinase 2

Names: TSK-2; TSSK-2; serine/threonine kinase 22B (spermiogenesis associated); serine/threonine-protein kinase 22B; testis specific serine/ threonine kinase 2; testis-specific kinase 2

NP_033462.2

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_009436.2 → NP_033462.2 testis-specific serine/threonine-protein kinase 2

See identical proteins and their annotated locations for NP_033462.2

Status: VALIDATED

Source sequence(s)

BC049618, BC061175, BY714760

Consensus CDS

CCDS28012.1

UniProtKB/Swiss-Prot

O54863, Q6P8M9

Related

ENSMUSP00000051035.7, ENSMUST00000055374.8

Conserved Domains (2) summary

smart00220
Location:12 → 272

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14165
Location:10 → 272

STKc_TSSK1_2-like; Catalytic domain of testis-specific serine/threonine kinase 1, TSSK2, and similar proteins