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    Spop speckle-type BTB/POZ protein [ Mus musculus (house mouse) ]

    Gene ID: 20747, updated on 21-Apr-2024

    Summary

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    Official Symbol
    Spopprovided by MGI
    Official Full Name
    speckle-type BTB/POZ proteinprovided by MGI
    Primary source
    MGI:MGI:1343085
    See related
    Ensembl:ENSMUSG00000057522 AllianceGenome:MGI:1343085
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    TEF2; Pcif1
    Summary
    Enables RNA polymerase II-specific DNA-binding transcription factor binding activity. Acts upstream of or within several processes, including glucose homeostasis; positive regulation of apoptotic process; and regulation of transcription, DNA-templated. Located in nuclear speck. Is expressed in lung; metanephros; and pancreas. Orthologous to human SPOP (speckle type BTB/POZ protein). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Ubiquitous expression in cerebellum adult (RPKM 32.0), bladder adult (RPKM 30.6) and 28 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    11 D; 11 59.01 cM
    Exon count:
    10
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 11 NC_000077.7 (95304909..95384236)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 11 NC_000077.6 (95414083..95493410)

    Chromosome 11 - NC_000077.7Genomic Context describing neighboring genes Neighboring gene family with sequence similarity 117, member A Neighboring gene STARR-positive B cell enhancer ABC_E3009 Neighboring gene CapStarr-seq enhancer MGSCv37_chr11:95253828-95254029 Neighboring gene STARR-seq mESC enhancer starr_30505 Neighboring gene solute carrier family 35, member B1 Neighboring gene STARR-positive B cell enhancer ABC_E3010 Neighboring gene STARR-positive B cell enhancer ABC_E10708 Neighboring gene predicted gene, 53646 Neighboring gene neurexophilin 3

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. The diverse roles of SPOP in prostate cancer and kidney cancer. Wang Z, et al. Nat Rev Urol, 2020 Jun. PMID 32355326
    2. SPOP and cancer: a systematic review. Clark A, et al. Am J Cancer Res, 2020. PMID 32266086, Free PMC Article
    3. The emerging role of SPOP protein in tumorigenesis and cancer therapy. Song Y, et al. Mol Cancer, 2020 Jan 4. PMID 31901237, Free PMC Article
    4. The ubiquitin ligase adaptor SPOP in cancer. Cuneo MJ, et al. FEBS J, 2019 Oct. PMID 31495053, Free PMC Article
    5. The E3 ubiquitin ligase SPOP controls resolution of systemic inflammation by triggering MYD88 degradation. Guillamot M, et al. Nat Immunol, 2019 Sep. PMID 31406379, Free PMC Article

    See all (63) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Substrate-dependent interaction of SPOP and RACK1 aggravates cardiac fibrosis following myocardial infarction.
      Title: Substrate-dependent interaction of SPOP and RACK1 aggravates cardiac fibrosis following myocardial infarction.
    2. Spop ameliorates diabetic nephropathy through restraining NLRP3 inflammasome.
      Title: Spop ameliorates diabetic nephropathy through restraining NLRP3 inflammasome.
    3. CHD1 and SPOP synergistically protect prostate epithelial cells from DNA damage.
      Title: CHD1 and SPOP synergistically protect prostate epithelial cells from DNA damage.
    4. Dual functions of SPOP and ERG dictate androgen therapy responses in prostate cancer.
      Title: Dual functions of SPOP and ERG dictate androgen therapy responses in prostate cancer.
    5. SPOP is a key regulator of emergency hematopoiesis and inflammation sensing in the bone marrow.
      Title: The E3 ubiquitin ligase SPOP controls resolution of systemic inflammation by triggering MYD88 degradation.
    6. SPOP is required for embryo implantation, endometrial decidualization, and uterine health in the mouse
      Title: Uterine function in the mouse requires speckle-type poz protein.
    7. no evidence that ERG is an effector of SPOP mutation in human prostate cancer or mouse models.
      Title: SPOP mutation drives prostate neoplasia without stabilizing oncogenic transcription factor ERG.
    8. loss of Spop, but not Spopl, disrupts chondrocyte hypertrophy and osteoblast differentiation in the mouse, suggesting the requirement for Spop-mediated protein degradation in mouse skeletal development; overexpressed Spop targets both Gli3FL and Gli3R for ubiquitination and degradation and Spop is an important positive regulator of Ihh signaling and skeletal development
      Title: Spop promotes skeletal development and homeostasis by positively regulating Ihh signaling.
    9. Results demonstrate a negative role of Spop in the level and activity of Gli3, Shh signaling and ventral spinal cord patterning.
      Title: Spop regulates Gli3 activity and Shh signaling in dorsoventral patterning of the mouse spinal cord.
    10. Speckle-type pox virus and zinc finger (POZ) protein (SPOP) regulates endometrial stromal cell decidualization in mice and that hormones regulate the expression of SPOP. This study suggests that ubiquitination may be involved in embryonic implantation.
      Title: SPOP Regulates Endometrial Stromal Cell Decidualization in Mice.
    Submit: New GeneRIF Correction See all GeneRIFs (16)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Gene trapped (1) 
    • Targeted (6)  1 citation
    • Endonuclease-mediated (1) 

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables RNA polymerase II-specific DNA-binding transcription factor binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables molecular function inhibitor activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables ubiquitin protein ligase binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ubiquitin protein ligase binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within glucose homeostasis IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in localization ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within negative regulation of DNA-binding transcription factor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of type B pancreatic cell apoptotic process IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in proteasome-mediated ubiquitin-dependent protein catabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within proteasome-mediated ubiquitin-dependent protein catabolic process IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in proteasome-mediated ubiquitin-dependent protein catabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein polyubiquitination ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in regulation of proteolysis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of type B pancreatic cell apoptotic process IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within ubiquitin-dependent protein catabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of Cul3-RING ubiquitin ligase complex ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nuclear speck IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    speckle-type POZ protein
    Names
    HIB homolog 1
    PDX-1 C-terminal-interacting factor 1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001359107.1 → NP_001346036.1  speckle-type POZ protein

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) uses alternate splice sites in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.
      Source sequence(s)
      AK132383, BF662657, BY172312
      Consensus CDS
      CCDS36286.1
      UniProtKB/Swiss-Prot
      Q3TLC2, Q5ST07, Q6ZWS8, Q76LV9
      UniProtKB/TrEMBL
      Q3THQ1
      Related
      ENSMUSP00000103350.2, ENSMUST00000107722.8
      Conserved Domains (3) summary
      cd03774
      Location:28 → 166
      MATH_SPOP; Speckle-type POZ protein (SPOP) family, MATH domain; composed of proteins with similarity to human SPOP. SPOP was isolated as a novel antigen recognized by serum from a scleroderma patient, whose overexpression in COS cells results in a discrete speckled ...
      cd18279
      Location:182 → 301
      BTB_POZ_SPOP-like; BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in speckle-type POZ protein (SPOP) and similar proteins
      cd18518
      Location:297 → 367
      BACK_SPOP; BACK (BTB and C-terminal Kelch) domain found in speckle-type POZ protein (SPOP)

    2. NM_025287.2 → NP_079563.2  speckle-type POZ protein

      See identical proteins and their annotated locations for NP_079563.2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.
      Source sequence(s)
      AB071989, AK004669, BY303667, BY726358, CJ207806
      Consensus CDS
      CCDS36286.1
      UniProtKB/Swiss-Prot
      Q3TLC2, Q5ST07, Q6ZWS8, Q76LV9
      UniProtKB/TrEMBL
      Q3THQ1
      Related
      ENSMUSP00000103352.3, ENSMUST00000107724.9
      Conserved Domains (3) summary
      cd03774
      Location:28 → 166
      MATH_SPOP; Speckle-type POZ protein (SPOP) family, MATH domain; composed of proteins with similarity to human SPOP. SPOP was isolated as a novel antigen recognized by serum from a scleroderma patient, whose overexpression in COS cells results in a discrete speckled ...
      cd18279
      Location:182 → 301
      BTB_POZ_SPOP-like; BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in speckle-type POZ protein (SPOP) and similar proteins
      cd18518
      Location:297 → 367
      BACK_SPOP; BACK (BTB and C-terminal Kelch) domain found in speckle-type POZ protein (SPOP)

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000077.7 Reference GRCm39 C57BL/6J

      Range
      95304909..95384236
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q6ZWS8.1 GenPept UniProtKB/Swiss-Prot:Q6ZWS8

    Gene LinkOut

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