Usp8 ubiquitin specific peptidase 8 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Usp8provided by MGI
Official Full Name: ubiquitin specific peptidase 8provided by MGI
Primary source: MGI:MGI:1934029
See related: Ensembl:ENSMUSG00000027363 AllianceGenome:MGI:1934029
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Ubpy; mKIAA0055
Summary: Enables thiol-dependent deubiquitinase. Involved in endosome organization and protein deubiquitination. Acts upstream of or within Ras protein signal transduction. Located in acrosomal membrane; cytosol; and midbody. Is extrinsic component of endosome membrane. Is expressed in several structures, including brain; genitourinary system; gut; hemolymphoid system gland; and sensory organ. Human ortholog(s) of this gene implicated in ACTH-secreting pituitary adenoma. Orthologous to human USP8 (ubiquitin specific peptidase 8). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in testis adult (RPKM 45.2), bladder adult (RPKM 14.4) and 25 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 F1; 2 61.76 cM

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (126549280..126601234)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (126707201..126759314)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

USP8-governed GPX4 homeostasis orchestrates ferroptosis and cancer immunotherapy.
Title: USP8-governed GPX4 homeostasis orchestrates ferroptosis and cancer immunotherapy.
Brucella targets the host ubiquitin-specific protease, Usp8, through the effector protein, TcpB, for facilitating infection of macrophages.
Title: Brucella targets the host ubiquitin-specific protease, Usp8, through the effector protein, TcpB, for facilitating infection of macrophages.
Inhibition of ubiquitin specific peptidase 8 is effective against 5-fluorouracil resistance in colon cancer via suppressing EGFR and EGFR-mediated signaling pathways.
Title: Inhibition of ubiquitin specific peptidase 8 is effective against 5-fluorouracil resistance in colon cancer via suppressing EGFR and EGFR-mediated signaling pathways.
Central role of ubiquitin-specific protease 8 in leptin signaling pathway in pulmonary arterial hypertension.
Title: Central role of ubiquitin-specific protease 8 in leptin signaling pathway in pulmonary arterial hypertension.
USP8 mitigates cognitive dysfunction of mice with sepsis-associated encephalopathy.
Title: USP8 mitigates cognitive dysfunction of mice with sepsis-associated encephalopathy.
Ubiquitin-Specific Peptidase 8 Is Critical for the Onset of Infectious Osteomyelitis by Targeting RIPK2 Ubiquitination.
Title: Ubiquitin-Specific Peptidase 8 Is Critical for the Onset of Infectious Osteomyelitis by Targeting RIPK2 Ubiquitination.
Ubiquitinspecific protease 8 ameliorates lipopolysaccharideinduced spleen injury via suppression of NFkappaB and MAPK signaling pathways.
Title: Ubiquitin‑specific protease 8 ameliorates lipopolysaccharide‑induced spleen injury via suppression of NF‑κB and MAPK signaling pathways.
USP8 inhibition reshapes an inflamed tumor microenvironment that potentiates the immunotherapy.
Title: USP8 inhibition reshapes an inflamed tumor microenvironment that potentiates the immunotherapy.
Deubiquitinating Enzyme USP8 Is Essential for Skeletogenesis by Regulating Wnt Signaling.
Title: Deubiquitinating Enzyme USP8 Is Essential for Skeletogenesis by Regulating Wnt Signaling.
Deubiquitination of EPG5 by USP8 guards the autophagic flux in embryonic stem cells (ESCs) to maintain their stemness. This work uncovers a novel crosstalk pathway between ubiquitination and autophagy through USP8-EPG5 interaction to regulate the stemness of ESCs.
Title: USP8 maintains embryonic stem cell stemness via deubiquitination of EPG5.

Submit: New GeneRIF Correction See all GeneRIFs (27)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Endonuclease-mediated (3)
Targeted (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AW557536 (e-PCR)
- UniSTS:178698

AI574262 (e-PCR)
- UniSTS:164177

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables K48-linked deubiquitinase activity	ISO Inferred from Sequence Orthology more info
enables K63-linked deubiquitinase activity	ISO Inferred from Sequence Orthology more info
enables SH3 domain binding	IEA Inferred from Electronic Annotation more info
enables cysteine-type deubiquitinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables cysteine-type deubiquitinase activity	IDA Inferred from Direct Assay more info	PubMed
enables cysteine-type deubiquitinase activity	ISO Inferred from Sequence Orthology more info
enables cysteine-type peptidase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables peptidase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in Ras protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within Ras protein signal transduction	IPI Inferred from Physical Interaction more info	PubMed
acts_upstream_of_or_within cell cycle	IEA Inferred from Electronic Annotation more info
involved_in cellular response to nerve growth factor stimulus	ISO Inferred from Sequence Orthology more info
involved_in endosome organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in endosome organization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within mitotic cytokinesis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of lysosomal protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of amyloid fibril formation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of canonical Wnt signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in protein K48-linked deubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein K48-linked deubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein K63-linked deubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein K63-linked deubiquitination	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein deubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein deubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein deubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within protein deubiquitination	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within proteolysis	IEA Inferred from Electronic Annotation more info
involved_in regulation of protein catabolic process at postsynapse, modulating synaptic transmission	ISO Inferred from Sequence Orthology more info
involved_in regulation of protein localization	ISO Inferred from Sequence Orthology more info
involved_in regulation of protein stability	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in acrosomal membrane	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in dendritic spine	IBA Inferred from Biological aspect of Ancestor more info
located_in dendritic spine	ISO Inferred from Sequence Orthology more info
colocalizes_with early endosome	ISO Inferred from Sequence Orthology more info
located_in early endosome	ISO Inferred from Sequence Orthology more info
located_in endosome	IEA Inferred from Electronic Annotation more info
located_in endosome membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in intracellular anatomical structure	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in midbody	IBA Inferred from Biological aspect of Ancestor more info
located_in midbody	IDA Inferred from Direct Assay more info	PubMed
located_in midbody	ISO Inferred from Sequence Orthology more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
is_active_in postsynaptic density	IBA Inferred from Biological aspect of Ancestor more info
is_active_in postsynaptic density	ISO Inferred from Sequence Orthology more info
located_in postsynaptic density	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: ubiquitin carboxyl-terminal hydrolase 8

Names: deubiquitinating enzyme 8; putative deubiquitinating enzyme; ubiquitin isopeptidase Y; ubiquitin thioesterase 8; ubiquitin thiolesterase 8; ubiquitin-specific-processing protease 8

NP_001239509.1

EC 3.4.19.12

NP_001409160.1

EC 3.4.19.12

NP_001409161.1

EC 3.4.19.12

NP_001409162.1

EC 3.4.19.12

NP_001409163.1

EC 3.4.19.12

NP_062703.2

EC 3.4.19.12

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001252580.2 → NP_001239509.1 ubiquitin carboxyl-terminal hydrolase 8 isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

Source sequence(s)

AL732330

Consensus CDS

CCDS57185.1

UniProtKB/TrEMBL

A2AI52, Q3UZ62

Related

ENSMUSP00000106046.3, ENSMUST00000110416.3

Conserved Domains (4) summary

smart00450
Location:210 → 321

RHOD; Rhodanese Homology Domain

cd02674
Location:751 → 1080

Peptidase_C19R; A subfamily of peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...

pfam00443
Location:749 → 1079

UCH; Ubiquitin carboxyl-terminal hydrolase

pfam08969
Location:6 → 126

USP8_dimer; USP8 dimerization domain
NM_001422231.1 → NP_001409160.1 ubiquitin carboxyl-terminal hydrolase 8 isoform 3

Status: VALIDATED

Source sequence(s)

AL732330
NM_001422232.1 → NP_001409161.1 ubiquitin carboxyl-terminal hydrolase 8 isoform 4

Status: VALIDATED

Source sequence(s)

AL732330
NM_001422233.1 → NP_001409162.1 ubiquitin carboxyl-terminal hydrolase 8 isoform 5

Status: VALIDATED

Source sequence(s)

AL732330
NM_001422234.1 → NP_001409163.1 ubiquitin carboxyl-terminal hydrolase 8 isoform 6

Status: VALIDATED

Source sequence(s)

AL732330
NM_019729.4 → NP_062703.2 ubiquitin carboxyl-terminal hydrolase 8 isoform 2

See identical proteins and their annotated locations for NP_062703.2

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

Source sequence(s)

AL732330

Consensus CDS

CCDS16687.1

UniProtKB/Swiss-Prot

A2AI53, Q80U87, Q80YP2, Q8R0D3, Q9EQU1, Q9WVP5

UniProtKB/TrEMBL

Q3UZ62

Related

ENSMUSP00000028841.8, ENSMUST00000028841.14

Conserved Domains (4) summary

smart00450
Location:199 → 310

RHOD; Rhodanese Homology Domain

cd02674
Location:740 → 1069

Peptidase_C19R; A subfamily of peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...

pfam00443
Location:738 → 1068

UCH; Ubiquitin carboxyl-terminal hydrolase

pfam08969
Location:6 → 115

USP8_dimer; USP8 dimerization domain