Limk1 LIM domain kinase 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Limk1provided by MGI
Official Full Name: LIM domain kinase 1provided by MGI
Primary source: MGI:MGI:104572
See related: Ensembl:ENSMUSG00000029674 AllianceGenome:MGI:104572
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Limk; KIZ-1; LIMK-1
Summary: This gene encodes a member of the LIM kinase family of proteins. This protein is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein also stimulates axon growth and may play a role in brain development. Homozygous knockout mice for this gene exhibit reduced bone mass, abnormal neuronal morphology and altered synaptic function. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
Expression: Ubiquitous expression in cerebellum adult (RPKM 15.2), whole brain E14.5 (RPKM 14.2) and 27 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 74.73 cM; 5 G2

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (134684893..134718713, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (134656039..134689859, complement)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

RhoA-LIMK Signaling Axis Reveals Rostral-Caudal Plane and Spatial Dysregulation in the Brain of Alzheimer's Disease Mouse Models.
Title: RhoA-LIMK Signaling Axis Reveals Rostral-Caudal Plane and Spatial Dysregulation in the Brain of Alzheimer's Disease Mouse Models.
NGPF2 triggers synaptic scaling up through ALK-LIMK-cofilin-mediated mechanisms.
Title: NGPF2 triggers synaptic scaling up through ALK-LIMK-cofilin-mediated mechanisms.
Overexpression of LIMK1 in hippocampal excitatory neurons improves synaptic plasticity and social recognition memory in APP/PS1 mice.
Title: Overexpression of LIMK1 in hippocampal excitatory neurons improves synaptic plasticity and social recognition memory in APP/PS1 mice.
LncRNA Rian ameliorates sevoflurane anesthesia-induced cognitive dysfunction through regulation of miR-143-3p/LIMK1 axis.
Title: LncRNA Rian ameliorates sevoflurane anesthesia-induced cognitive dysfunction through regulation of miR-143-3p/LIMK1 axis.
Spine impairment in mice high-expressing neuregulin 1 due to LIMK1 activation.
Title: Spine impairment in mice high-expressing neuregulin 1 due to LIMK1 activation.
Lim Kinase1 regulates seizure activity via modulating actin dynamics.
Title: Lim Kinase1 regulates seizure activity via modulating actin dynamics.
Up-regulation of miR-106a targets LIMK1 and contributes to cognitive impairment induced by isoflurane anesthesia in mice.
Title: Up-regulation of miR-106a targets LIMK1 and contributes to cognitive impairment induced by isoflurane anesthesia in mice.
Deletion of Limk1 and Limk2 in mice does not alter cochlear development or auditory function.
Title: Deletion of Limk1 and Limk2 in mice does not alter cochlear development or auditory function.
Elevated cofilin activity, through the loss of Limk1, results in faster sciatic nerve growth following sciatic nerve injury.
Title: The Cofilin/Limk1 Pathway Controls the Growth Rate of Both Developing and Regenerating Motor Axons.
LIMK1 KO and LIMK2 KO mice were used to investigate the role of LIMK signaling in the cerebral cortical development.
Title: LIMK1 and LIMK2 regulate cortical development through affecting neural progenitor cell proliferation and migration.

Submit: New GeneRIF Correction See all GeneRIFs (44)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (3) 1 citation
Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

G48154 (e-PCR)
- UniSTS:95259

Limk1 (e-PCR), detects polymorphism
- UniSTS:527404

X86569 (e-PCR)
- UniSTS:179285

UniSTS:230793 (e-PCR)
- UniSTS:230793

Limk1 (e-PCR), detects polymorphism
- UniSTS:230793

Limk1 (e-PCR), detects polymorphism
- UniSTS:143779

G48114 (e-PCR)
- UniSTS:95224

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables heat shock protein binding	ISO Inferred from Sequence Orthology more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in actin cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of axon extension	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of ubiquitin-protein transferase activity	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of actin filament bundle assembly	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of axon extension	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of stress fiber assembly	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within positive regulation of stress fiber assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of stress fiber assembly	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in axonal growth cone	ISO Inferred from Sequence Orthology more info
located_in cell projection	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in focal adhesion	IDA Inferred from Direct Assay more info	PubMed
located_in lamellipodium	IDA Inferred from Direct Assay more info	PubMed
located_in male germ cell nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in neuron projection	IBA Inferred from Biological aspect of Ancestor more info
located_in neuron projection	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear speck	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: LIM domain kinase 1

Names: LIM-domain containing, protein kinase

NP_001292804.1

EC 2.7.11.1

NP_034847.1

EC 2.7.11.1

XP_036020724.1

EC 2.7.11.1

XP_036020725.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001305875.1 → NP_001292804.1 LIM domain kinase 1 isoform 2

See identical proteins and their annotated locations for NP_001292804.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) lacks two alternate exons and contains an alternate 5' terminal exon, resulting in the use of an alternate translation start site, compared to variant 1. The encoded isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1.

Source sequence(s)

AC166938, AK141813, BQ828670, BX527775, BY230595, CV563459

Consensus CDS

CCDS84969.1

UniProtKB/TrEMBL

E9PVB6, Q3TA53

Related

ENSMUSP00000106864.2, ENSMUST00000111233.8

Conserved Domains (5) summary

cd09464
Location:76 → 130

LIM2_LIMK1; The second LIM domain of LIMK1 (LIM domain Kinase 1)

smart00219
Location:334 → 596

TyrKc; Tyrosine kinase, catalytic domain

cd14221
Location:337 → 603

STKc_LIMK1; Catalytic domain of the Serine/Threonine Kinase, LIM domain kinase 1

pfam00595
Location:157 → 247

PDZ; PDZ domain (Also known as DHR or GLGF)

cl02475
Location:43 → 69

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers
NM_010717.3 → NP_034847.1 LIM domain kinase 1 isoform 1

See identical proteins and their annotated locations for NP_034847.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) encodes the longer isoform (1).

Source sequence(s)

AK141813, BQ828670, BX527775, CJ066818, CV563459

Consensus CDS

CCDS19724.1

UniProtKB/Swiss-Prot

P53668

UniProtKB/TrEMBL

Q3UR47

Related

ENSMUSP00000015137.4, ENSMUST00000015137.10

Conserved Domains (5) summary

cd09462
Location:5 → 77

LIM1_LIMK1; The first LIM domain of LIMK1 (LIM domain Kinase 1)

cd09464
Location:84 → 138

LIM2_LIMK1; The second LIM domain of LIMK1 (LIM domain Kinase 1)

smart00219
Location:342 → 604

TyrKc; Tyrosine kinase, catalytic domain

cd14221
Location:345 → 611

STKc_LIMK1; Catalytic domain of the Serine/Threonine Kinase, LIM domain kinase 1

pfam00595
Location:165 → 255

PDZ; PDZ domain (Also known as DHR or GLGF)

RNA

NR_131247.1 RNA Sequence

Status: REVIEWED

Description

Transcript Variant: This variant (3) has multiple differences, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AC166938, AK141813, BQ828670, BX527775, BY230595, CV563459

Related

ENSMUST00000134093.2

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000071.7 Reference GRCm39 C57BL/6J

Range

134684893..134718713 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036164831.1 → XP_036020724.1 LIM domain kinase 1 isoform X1

UniProtKB/TrEMBL

Q3UR47

Conserved Domains (4) summary

cd09462
Location:5 → 77

LIM1_LIMK1; The first LIM domain of LIMK1 (LIM domain Kinase 1)

cd09464
Location:84 → 138

LIM2_LIMK1; The second LIM domain of LIMK1 (LIM domain Kinase 1)

cd14221
Location:345 → 666

STKc_LIMK1; Catalytic domain of the Serine/Threonine Kinase, LIM domain kinase 1

pfam00595
Location:165 → 255

PDZ; PDZ domain (Also known as DHR or GLGF)
XM_036164832.1 → XP_036020725.1 LIM domain kinase 1 isoform X2

UniProtKB/TrEMBL

Q3TA53

Conserved Domains (4) summary

cd09464
Location:76 → 130

LIM2_LIMK1; The second LIM domain of LIMK1 (LIM domain Kinase 1)

cd14221
Location:337 → 658

STKc_LIMK1; Catalytic domain of the Serine/Threonine Kinase, LIM domain kinase 1

pfam00595
Location:157 → 247

PDZ; PDZ domain (Also known as DHR or GLGF)

cl02475
Location:43 → 69

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers