Pde5a phosphodiesterase 5A, cGMP-specific [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Pde5aprovided by MGI
Official Full Name: phosphodiesterase 5A, cGMP-specificprovided by MGI
Primary source: MGI:MGI:2651499
See related: Ensembl:ENSMUSG00000053965 AllianceGenome:MGI:2651499
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Cn5n; Pde5; Cgbpde; PDE5a2; Pde5a1
Summary: Enables 3',5'-cyclic-nucleotide phosphodiesterase activity and cGMP binding activity. Involved in positive regulation of MAP kinase activity; regulation of muscle system process; and relaxation of cardiac muscle. Acts upstream of or within several processes, including cGMP catabolic process; negative regulation of T cell proliferation; and positive regulation of oocyte development. Predicted to be located in cytosol. Is expressed in several structures, including alimentary system; brain; genitourinary system; respiratory system; and sensory organ. Human ortholog(s) of this gene implicated in IgA glomerulonephritis. Orthologous to human PDE5A (phosphodiesterase 5A). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in bladder adult (RPKM 49.4), lung adult (RPKM 12.8) and 6 other tissues See more
Orthologs: human all
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Genomic context

Location:: 3 G1; 3 53.69 cM

Exon count:: 22

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	3	NC_000069.7 (122522822..122653023)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	3	NC_000069.6 (122729158..122859374)

Chromosome 3 - NC_000069.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Structural Characterization of Murine Phosphodiesterase 5 Isoforms and Involvement of Cysteine Residues in Supramolecular Assembly.
Title: Structural Characterization of Murine Phosphodiesterase 5 Isoforms and Involvement of Cysteine Residues in Supramolecular Assembly.
Exogenous PDE5 Expression Rescues Photoreceptors in RD1 Mice.
Title: Exogenous PDE5 Expression Rescues Photoreceptors in RD1 Mice.
Phosphodiesterase-5a Knock-out Suppresses Inflammation by Down-Regulating Adhesion Molecules in Cardiac Rupture Following Myocardial Infarction.
Title: Phosphodiesterase-5a Knock-out Suppresses Inflammation by Down-Regulating Adhesion Molecules in Cardiac Rupture Following Myocardial Infarction.
Inhibition of Phosphodiesterase 5 Promotes the Aromatase-Mediated Estrogen Biosynthesis in Osteoblastic Cells by Activation of cGMP/PKG/SHP2 Pathway.
Title: Inhibition of Phosphodiesterase 5 Promotes the Aromatase-Mediated Estrogen Biosynthesis in Osteoblastic Cells by Activation of cGMP/PKG/SHP2 Pathway.
Low PDE% expression is associated with sickle cell disease.
Title: Dysregulated NO/PDE5 signaling in the sickle cell mouse lower urinary tract: Reversal by oral nitrate therapy.
The aim of this study is to investigate the distribution of adipose tissue (AT) and the composition of the stroma-vascular fraction (SVF) of subcutaneous AT (SAT) in type 2 diabetic mice after prolonged treatment with a PDE5 inhibitor, Sildenafil.
Title: Chronic phosphodiesterase type 5 inhibition has beneficial effects on subcutaneous adipose tissue plasticity in type 2 diabetic mice.
analysis of the mechanisms involved revealed the induction of bone morphogenetic protein-7 (BMP7) expression, a critical regulator of angiogenesis and kidney homeostasis, through a PDE5i-dependent downregulation of miR-22. In conclusion PDE5i slows the progression of Diabetic Nephropathy (DN) in mice, improving hemodynamic parameters and vessel integrity.
Title: Phosphodiesterase-5 inhibition preserves renal hemodynamics and function in mice with diabetic kidney disease by modulating miR-22 and BMP7.
the activation of PDE5 is among the mechanisms contributing to the cGMP decrease, these results suggest that another cGMP phosphodiesterase is also activated by LH signaling.
Title: Luteinizing hormone signaling phosphorylates and activates the cyclic GMP phosphodiesterase PDE5 in mouse ovarian follicles, contributing an additional component to the hormonally induced decrease in cyclic GMP that reinitiates meiosis.
Report expression of Pde5a1/2/3 in cardiac myocytes and suggest role in induction of cardiac hypertrophy.
Title: Identification of murine phosphodiesterase 5A isoforms and their functional characterization in HL-1 cardiac cell line.
Upon S-nitrosylation, PDE5 exhibits reduced activity and degradation via the ubiquitin-proteasome system.
Title: S-nitrosylation of PDE5 increases its ubiquitin-proteasomal degradation.

Submit: New GeneRIF Correction See all GeneRIFs (36)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (1)
Endonuclease-mediated (5) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Pde5a (e-PCR), detects polymorphism
- UniSTS:546924

Results_ER_9_1_95_3_11.seq (e-PCR)
- UniSTS:125591

BB387693 (e-PCR)
- UniSTS:207561

Pde5a (e-PCR), detects polymorphism
- UniSTS:507126

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 3',5'-cyclic-AMP phosphodiesterase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 3',5'-cyclic-GMP phosphodiesterase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 3',5'-cyclic-GMP phosphodiesterase activity	ISO Inferred from Sequence Orthology more info
enables 3',5'-cyclic-nucleotide phosphodiesterase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables cGMP binding	IDA Inferred from Direct Assay more info	PubMed
enables catalytic activity	IEA Inferred from Electronic Annotation more info
enables cyclic-nucleotide phosphodiesterase activity	IDA Inferred from Direct Assay more info	PubMed
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables phosphoric diester hydrolase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of T cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cAMP-mediated signaling	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within cGMP catabolic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within cGMP metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within negative regulation of T cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cardiac muscle contraction	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of oocyte development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of oocyte development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of G protein-coupled receptor signaling pathway	IC Inferred by Curator more info	PubMed
involved_in positive regulation of MAP kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cardiac muscle hypertrophy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of chronic inflammatory response	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of oocyte development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within positive regulation of oocyte development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of vasoconstriction	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of nitric oxide mediated signal transduction	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of the force of heart contraction	ISO Inferred from Sequence Orthology more info
involved_in relaxation of cardiac muscle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in short-term memory	ISO Inferred from Sequence Orthology more info
involved_in signal transduction	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: cGMP-specific 3',5'-cyclic phosphodiesterase

Names: cGMP-binding cGMP-specific phosphodiesterase

NP_001396722.1

EC 3.1.4.35

NP_700471.2

EC 3.1.4.35

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001409793.1 → NP_001396722.1 cGMP-specific 3',5'-cyclic phosphodiesterase isoform 2

Status: VALIDATED

Source sequence(s)

AC101922, AC155158

UniProtKB/TrEMBL

A0A0G2JF67

Related

ENSMUSP00000143042.2, ENSMUST00000200389.2
NM_153422.3 → NP_700471.2 cGMP-specific 3',5'-cyclic phosphodiesterase isoform 1

See identical proteins and their annotated locations for NP_700471.2

Status: VALIDATED

Source sequence(s)

AC101922, AC155158

Consensus CDS

CCDS17812.1

UniProtKB/Swiss-Prot

Q0VBW0, Q8CG03

Related

ENSMUSP00000069011.6, ENSMUST00000066728.10

Conserved Domains (2) summary

smart00065
Location:154 → 311

GAF; Domain present in phytochromes and cGMP-specific phosphodiesterases

pfam00233
Location:602 → 837

PDEase_I; 3'5'-cyclic nucleotide phosphodiesterase