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    Ppp1r16b protein phosphatase 1, regulatory subunit 16B [ Mus musculus (house mouse) ]

    Gene ID: 228852, updated on 5-Mar-2024

    Summary

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    Official Symbol
    Ppp1r16bprovided by MGI
    Official Full Name
    protein phosphatase 1, regulatory subunit 16Bprovided by MGI
    Primary source
    MGI:MGI:2151841
    See related
    Ensembl:ENSMUSG00000037754 AllianceGenome:MGI:2151841
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Wdt4; TIMAP; ANKRD4; C130078N17Rik
    Summary
    Predicted to enable myosin phosphatase regulator activity and protein phosphatase 1 binding activity. Acts upstream of or within establishment of endothelial barrier. Predicted to be located in cell projection; nuclear speck; and perinuclear region of cytoplasm. Predicted to be active in plasma membrane. Is expressed in several structures, including brain; eye; heart; hemolymphoid system; and spinal cord. Orthologous to human PPP1R16B (protein phosphatase 1 regulatory subunit 16B). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Broad expression in thymus adult (RPKM 16.6), lung adult (RPKM 14.9) and 17 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    2 H1; 2 78.72 cM
    Exon count:
    13
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 2 NC_000068.8 (158508653..158608254)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 2 NC_000068.7 (158666733..158766334)

    Chromosome 2 - NC_000068.8Genomic Context describing neighboring genes Neighboring gene predicted gene 14204 Neighboring gene STARR-positive B cell enhancer ABC_E4515 Neighboring gene solute carrier family 32 (GABA vesicular transporter), member 1 Neighboring gene ARP5 actin-related protein 5 Neighboring gene STARR-positive B cell enhancer ABC_E10158 Neighboring gene microRNA 3474 Neighboring gene STARR-positive B cell enhancer ABC_E7879 Neighboring gene STARR-positive B cell enhancer mm9_chr2:158499319-158499620 Neighboring gene STARR-positive B cell enhancer ABC_E2650 Neighboring gene STARR-positive B cell enhancer ABC_E7880 Neighboring gene CapStarr-seq enhancer MGSCv37_chr2:158548828-158549026 Neighboring gene STARR-positive B cell enhancer ABC_E2056 Neighboring gene STARR-positive B cell enhancer ABC_E7881 Neighboring gene STARR-positive B cell enhancer ABC_E416 Neighboring gene family with sequence similarity 83, member D Neighboring gene STARR-seq mESC enhancer starr_06234 Neighboring gene DEAH-box helicase 35 Neighboring gene STARR-seq mESC enhancer starr_06235

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. mPPP1R16B is a novel mouse protein phosphatase 1 targeting subunit whose mRNA is located in cell bodies and dendrites of neurons in four distinct regions of the brain. Magdaleno S, et al. Brain Res Gene Expr Patterns, 2002 Oct. PMID 12638124
    2. TIMAP repression by TGFβ and HDAC3-associated Smad signaling regulates macrophage M2 phenotypic phagocytosis. Yang J, et al. J Mol Med (Berl), 2017 Mar. PMID 27709267
    3. TIMAP inhibits endothelial myosin light chain phosphatase by competing with MYPT1 for the catalytic protein phosphatase 1 subunit PP1cβ. Wang X, et al. J Biol Chem, 2019 Sep 6. PMID 31315927, Free PMC Article
    4. TIMAP protects endothelial barrier from LPS-induced vascular leakage and is down-regulated by LPS. Poirier C, et al. Respir Physiol Neurobiol, 2011 Dec 15. PMID 21907835, Free PMC Article
    5. TIMAP, a novel CAAX box protein regulated by TGF-beta1 and expressed in endothelial cells. Cao W, et al. Am J Physiol Cell Physiol, 2002 Jul. PMID 12055102

    See all (23) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. TIMAP is suppressed by TGFbeta via HDAC3-associated Smad signaling.
      Title: TIMAP repression by TGFβ and HDAC3-associated Smad signaling regulates macrophage M2 phenotypic phagocytosis.
    2. These data confirmed the barrier-protective role of TIMAP
      Title: TIMAP protects endothelial barrier from LPS-induced vascular leakage and is down-regulated by LPS.
    3. mRNA is located in cell bodies and dendrites of neurons in four distinct regions of the brain.
      Title: mPPP1R16B is a novel mouse protein phosphatase 1 targeting subunit whose mRNA is located in cell bodies and dendrites of neurons in four distinct regions of the brain.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Targeted (1)  1 citation
    • Endonuclease-mediated (1) 

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables enzyme inhibitor activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables myosin phosphatase regulator activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables protein phosphatase regulator activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    involved_in establishment of endothelial barrier IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within establishment of endothelial barrier IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in establishment of endothelial barrier ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in negative regulation of peptidyl-serine dephosphorylation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in negative regulation of protein dephosphorylation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in positive regulation of endothelial cell proliferation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in positive regulation of protein dephosphorylation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in regulation of protein dephosphorylation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in regulation of sprouting angiogenesis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    located_in cell projection ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nuclear speck ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in nucleus ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in perinuclear region of cytoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in plasma membrane ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    protein phosphatase 1 regulatory inhibitor subunit 16B
    Names
    CAAX box protein TIMAP
    TGF-beta-inhibited membrane-associated protein
    protein phosphatase 1, regulatory (inhibitor) subunit 16B
    whn-dependent transcript 4

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001159662.1 → NP_001153134.1  protein phosphatase 1 regulatory inhibitor subunit 16B

      See identical proteins and their annotated locations for NP_001153134.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) represents the longer transcript. Both variants encode the same protein.
      Source sequence(s)
      AK037627, AL663077, BY718617
      Consensus CDS
      CCDS16991.1
      UniProtKB/Swiss-Prot
      Q8VHQ3
      UniProtKB/TrEMBL
      A2AC90, Q544H9
      Related
      ENSMUSP00000039540.5, ENSMUST00000045503.11
      Conserved Domains (3) summary
      PHA03095
      Location:71 → 301
      PHA03095; ankyrin-like protein; Provisional
      sd00045
      Location:71 → 98
      ANK; ANK repeat [structural motif]
      pfam12796
      Location:72 → 164
      Ank_2; Ankyrin repeats (3 copies)

    2. NM_153089.4 → NP_694729.1  protein phosphatase 1 regulatory inhibitor subunit 16B

      See identical proteins and their annotated locations for NP_694729.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants encode the same protein.
      Source sequence(s)
      AK037627, AL663077
      Consensus CDS
      CCDS16991.1
      UniProtKB/Swiss-Prot
      Q8VHQ3
      UniProtKB/TrEMBL
      A2AC90, Q544H9
      Related
      ENSMUSP00000099405.4, ENSMUST00000103116.10
      Conserved Domains (3) summary
      PHA03095
      Location:71 → 301
      PHA03095; ankyrin-like protein; Provisional
      sd00045
      Location:71 → 98
      ANK; ANK repeat [structural motif]
      pfam12796
      Location:72 → 164
      Ank_2; Ankyrin repeats (3 copies)

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000068.8 Reference GRCm39 C57BL/6J

      Range
      158508653..158608254
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_011239493.3 → XP_011237795.1  protein phosphatase 1 regulatory inhibitor subunit 16B isoform X2

      Conserved Domains (3) summary
      PHA03095
      Location:71 → 301
      PHA03095; ankyrin-like protein; Provisional
      sd00045
      Location:71 → 98
      ANK; ANK repeat [structural motif]
      pfam12796
      Location:72 → 164
      Ank_2; Ankyrin repeats (3 copies)

    2. XM_011239492.4 → XP_011237794.1  protein phosphatase 1 regulatory inhibitor subunit 16B isoform X1

      See identical proteins and their annotated locations for XP_011237794.1

      UniProtKB/Swiss-Prot
      Q8VHQ3
      UniProtKB/TrEMBL
      A2AC90, Q544H9
      Related
      ENSMUSP00000117310.2, ENSMUST00000145073.2
      Conserved Domains (3) summary
      PHA03095
      Location:71 → 301
      PHA03095; ankyrin-like protein; Provisional
      sd00045
      Location:71 → 98
      ANK; ANK repeat [structural motif]
      pfam12796
      Location:72 → 164
      Ank_2; Ankyrin repeats (3 copies)
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q8VHQ3.1 GenPept UniProtKB/Swiss-Prot:Q8VHQ3

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