Dars1 aspartyl-tRNA synthetase 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Dars1provided by MGI
Official Full Name: aspartyl-tRNA synthetase 1provided by MGI
Primary source: MGI:MGI:2442544
See related: Ensembl:ENSMUSG00000026356 AllianceGenome:MGI:2442544
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Dars; 5730439G15Rik
Summary: Predicted to enable RNA binding activity and aspartate-tRNA ligase activity. Predicted to be involved in aspartyl-tRNA aminoacylation. Predicted to act upstream of or within translation. Located in cytoplasm and synapse. Part of aminoacyl-tRNA synthetase multienzyme complex. Is expressed in several structures, including alimentary system; brain; genitourinary system; respiratory system; and sensory organ. Orthologous to human DARS1 (aspartyl-tRNA synthetase 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in CNS E11.5 (RPKM 27.3), placenta adult (RPKM 26.4) and 25 other tissues See more
Orthologs: human all
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Genomic context

Location:: 1 E3; 1 55.8 cM

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	1	NC_000067.7 (128291444..128345153, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	1	NC_000067.6 (128363707..128417416, complement)

Chromosome 1 - NC_000067.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Study provides an in-depth characterization of the DARS tissue distribution in mice revealing surprising heterogeneity across brain regions and neural cell types, addresses the paucity of knowledge around Dars gene expression, cell and systems physiology and associated pathophysiology related to the leukodystrophy Hypomyelination with Brain stem and Spinal cord involvement and Leg spasticity.
Title: In vivocharacterization of the aspartyl-tRNA synthetase DARS: Homing in on the leukodystrophy HBSL.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (2) 1 citation
Endonuclease-mediated (3)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Dars (e-PCR), detects polymorphism
- UniSTS:506835

NoName (e-PCR)
- UniSTS:234207

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables RNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables aminoacyl-tRNA ligase activity	IEA Inferred from Electronic Annotation more info
enables aspartate-tRNA ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables aspartate-tRNA ligase activity	ISO Inferred from Sequence Orthology more info
enables ligase activity	IEA Inferred from Electronic Annotation more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in aspartyl-tRNA aminoacylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in aspartyl-tRNA aminoacylation	ISO Inferred from Sequence Orthology more info
involved_in tRNA aminoacylation for protein translation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within translation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of aminoacyl-tRNA synthetase multienzyme complex	IBA Inferred from Biological aspect of Ancestor more info
part_of aminoacyl-tRNA synthetase multienzyme complex	IDA Inferred from Direct Assay more info	PubMed
part_of aminoacyl-tRNA synthetase multienzyme complex	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in synapse	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: aspartate--tRNA ligase, cytoplasmic

Names: aspRS; aspartyl-tRNA synthetase, cytoplasmic

NP_663482.2

EC 6.1.1.12

NP_803228.2

EC 6.1.1.12

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_145507.2 → NP_663482.2 aspartate--tRNA ligase, cytoplasmic isoform 2

See identical proteins and their annotated locations for NP_663482.2

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 3' UTR and includes an alternate 3' exon, but it lacks several exons in the 3' coding region compared to variant 1. The resulting isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1.

Source sequence(s)

AK077820, BB576692, BY308833

Consensus CDS

CCDS48348.1

UniProtKB/TrEMBL

Q8BJY7

Related

ENSMUSP00000066866.8, ENSMUST00000064309.9

Conserved Domains (1) summary

cl09930
Location:60 → 72

RPA_2b-aaRSs_OBF_like; Replication protein A, class 2b aminoacyl-tRNA synthetases, and related proteins with oligonucleotide/oligosaccharide (OB) fold.
NM_177445.5 → NP_803228.2 aspartate--tRNA ligase, cytoplasmic isoform 1

See identical proteins and their annotated locations for NP_803228.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AK151027, BY276340, CO043663

Consensus CDS

CCDS15253.1

UniProtKB/Swiss-Prot

Q3U987, Q922B2

UniProtKB/TrEMBL

Q3TF87, Q3TMC5, Q8BK18

Related

ENSMUSP00000027602.9, ENSMUST00000027602.15

Conserved Domains (3) summary

PLN02850
Location:20 → 501

PLN02850; aspartate-tRNA ligase

cd00776
Location:174 → 497

AsxRS_core; Asx tRNA synthetase (AspRS/AsnRS) class II core domain. Assignment to class II aminoacyl-tRNA synthetases (aaRS) based upon its structure and the presence of three characteristic sequence motifs in the core domain. This family includes AsnRS as well as ...

cd04320
Location:60 → 158

AspRS_cyto_N; AspRS_cyto_N: N-terminal, anticodon recognition domain of the type found in Saccharomyces cerevisiae and human cytoplasmic aspartyl-tRNA synthetase (AspRS). This domain is a beta-barrel domain (OB fold) involved in binding the tRNA anticodon stem-loop. ...