Nisch nischarin [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Nischprovided by MGI
Official Full Name: nischarinprovided by MGI
Primary source: MGI:MGI:1928323
See related: Ensembl:ENSMUSG00000021910 AllianceGenome:MGI:1928323
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: I-1; edsn; 1200007D05Rik; 3202002H23Rik
Summary: Enables integrin binding activity. Acts upstream of or within Rac protein signal transduction; actin cytoskeleton organization; and negative regulation of cell migration. Located in cytosol. Is expressed in several structures, including alimentary system; brain; cardiovascular system; genitourinary system; and integumental system. Used to study otitis media. Human ortholog(s) of this gene implicated in congestive heart failure. Orthologous to human NISCH (nischarin). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in ovary adult (RPKM 82.3), adrenal adult (RPKM 79.3) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 14 B; 14 19.09 cM

Exon count:: 23

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	14	NC_000080.7 (30892885..30928783, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	14	NC_000080.6 (31170928..31206826, complement)

Chromosome 14 - NC_000080.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Contribution of Nischarin/IRAS in CNS development, injury and diseases.
Title: Contribution of Nischarin/IRAS in CNS development, injury and diseases.
Identification of a psychiatric risk gene NISCH at 3p21.1 GWAS locus mediating dendritic spine morphogenesis and cognitive function.
Title: Identification of a psychiatric risk gene NISCH at 3p21.1 GWAS locus mediating dendritic spine morphogenesis and cognitive function.
Nischarin Deletion Reduces Oxidative Metabolism and Overall ATP: A Study Using a Novel NISCH(Delta5-6) Knockout Mouse Model.
Title: Nischarin Deletion Reduces Oxidative Metabolism and Overall ATP: A Study Using a Novel NISCH(Δ5-6) Knockout Mouse Model.
Expression of nischarin, an imidazoline 1 receptor candidate protein, in the ventrolateral medulla of newborn rats.
Title: Expression of nischarin, an imidazoline 1 receptor candidate protein, in the ventrolateral medulla of newborn rats.
These data suggest that IRAS/Nischarin may play an important role in the development of morphine tolerance and physical dependence in vivo through modulating MOR expression, as well as AMPA GluR1-S845 phosphorylation, which might be one of the mechanisms underlying the development of opiate addiction.
Title: The role of IRAS/Nischarin involved in the development of morphine tolerance and physical dependence.
results indicate that Nisch is an important AMPK inhibitor and a critical regulator of energy homeostasis, including lipid and glucose metabolism.
Title: Nischarin inhibition alters energy metabolism by activating AMP-activated protein kinase.
We identified a significant genetic interaction between Nisch and Itga5; mice heterozygous for Itga5-null and homozygous for edison mutations display a significantly increased penetrance and severity of chronic otitis media.
Title: A mutation in Nischarin causes otitis media via LIMK1 and NF-κB pathways.
Nischarin inhibits neurite outgrowth by blocking PAK1 activation in neurons.
Title: Inhibition of Nischarin Expression Promotes Neurite Outgrowth through Regulation of PAK Activity.
Data from studies using various receptor antagonists/agonists suggest that imidazoline receptors (Nisch I-1), alpha2-adrenoceptors, and endothelin-A receptors are involved in body temperature regulation in mice.
Title: Involvement of α₂-adrenoceptors, imidazoline, and endothelin-A receptors in the effect of agmatine on morphine and oxycodone-induced hypothermia in mice.
Activation of I1-imidazoline receptor ameliorated energy intake and epididymal white adipose tissue accumulation.
Title: Improvement of obesity by activation of I1-imidazoline receptors in high fat diet-fed mice.

Submit: New GeneRIF Correction See all GeneRIFs (18)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (7) 1 citation
Chemically induced (ENU) (2) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D14Mit13 (e-PCR), detects polymorphism
- UniSTS:127235

NoName (e-PCR)
- UniSTS:237151

NoName (e-PCR)
- UniSTS:237153

NoName (e-PCR)
- UniSTS:237154

AW494485 (e-PCR)
- UniSTS:180623

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables alpha-tubulin binding	IBA Inferred from Biological aspect of Ancestor more info
enables dynein heavy chain binding	IBA Inferred from Biological aspect of Ancestor more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables integrin binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within Rac protein signal transduction	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within actin cytoskeleton organization	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in glucose metabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of cell migration	IDA Inferred from Direct Assay more info	PubMed
involved_in norepinephrine secretion	ISO Inferred from Sequence Orthology more info
involved_in outer dynein arm assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of blood pressure	ISO Inferred from Sequence Orthology more info
involved_in regulation of synaptic transmission, GABAergic	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in endosome	IEA Inferred from Electronic Annotation more info
located_in intercellular bridge	ISO Inferred from Sequence Orthology more info
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in microtubule cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: nischarin

Names: I1R; IR1; imidazoline receptor 1; imidazoline receptor I-1-like protein; imidazoline-1 receptor

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001347583.1 → NP_001334512.1 nischarin isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks multiple exons and includes an alternate 3' terminal exon, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1.

Source sequence(s)

AC154446, AK004628, AK160990, BY602009, CJ106892

Consensus CDS

CCDS84107.1

UniProtKB/TrEMBL

Q8CF63

Related

ENSMUSP00000132413.2, ENSMUST00000172142.8
NM_022656.2 → NP_073147.2 nischarin isoform 1

See identical proteins and their annotated locations for NP_073147.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AC154446, AK122412, BC089329, BP761152, CJ106892

Consensus CDS

CCDS36853.1

UniProtKB/Swiss-Prot

Q2YDV7, Q80TM9, Q8C354, Q8C4X9, Q8CBH0, Q91XW6, Q99LG6, Q9EPW8, Q9WUM6

UniProtKB/TrEMBL

B7ZN33

Related

ENSMUSP00000022469.7, ENSMUST00000022469.13

Conserved Domains (5) summary

cd06875
Location:15 → 130

PX_IRAS; The phosphoinositide binding Phox Homology domain of the Imidazoline Receptor Antisera-Selected

cd00116
Location:271 → 417

LRR_RI; Leucine-rich repeats (LRRs), ribonuclease inhibitor (RI)-like subfamily. LRRs are 20-29 residue sequence motifs present in many proteins that participate in protein-protein interactions and have different functions and cellular locations. LRRs correspond ...

sd00033
Location:291 → 313

LRR_RI; leucine-rich repeat [structural motif]

pfam12799
Location:312 → 352

LRR_4; Leucine Rich repeats (2 copies)

pfam13855
Location:291 → 346

LRR_8; Leucine rich repeat