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    Pfkfb3 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 [ Rattus norvegicus (Norway rat) ]

    Gene ID: 117276, updated on 11-Apr-2024

    Summary

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    Official Symbol
    Pfkfb3provided by RGD
    Official Full Name
    6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3provided by RGD
    Primary source
    RGD:619776
    See related
    Ensembl:ENSRNOG00000018911 AllianceGenome:RGD:619776
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Rattus norvegicus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
    Summary
    Predicted to enable 6-phosphofructo-2-kinase activity and fructose-2,6-bisphosphate 2-phosphatase activity. Involved in brain development and fructose 2,6-bisphosphate metabolic process. Predicted to be located in nucleoplasm. Predicted to be active in cytosol. Orthologous to human PFKFB3 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Biased expression in Muscle (RPKM 207.9), Adrenal (RPKM 100.3) and 8 other tissues See more
    Orthologs
    human mouse all
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    Genomic context

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    Location:
    17q12.2
    Exon count:
    19
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCr8 (GCF_036323735.1) 17 NC_086035.1 (71893320..71974526)
    RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 17 NC_051352.1 (66983629..67064702)
    106 previous assembly Rnor_6.0 (GCF_000001895.5) 17 NC_005116.4 (70632778..70713736)

    Chromosome 17 - NC_086035.1Genomic Context describing neighboring genes Neighboring gene interleukin 2 receptor subunit alpha Neighboring gene proteasome 26S subunit, ATPase 6, pseudogene 1 Neighboring gene RNA binding motif protein 17 Neighboring gene U6 spliceosomal RNA Neighboring gene uncharacterized LOC120097756 Neighboring gene uncharacterized LOC134482599 Neighboring gene protein kinase C, theta Neighboring gene uncharacterized LOC134482595

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
    • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
    • BioProject: PRJNA238328
    • Publication: PMID 24510058
    • Analysis date: Mon Jun 6 17:44:12 2016

    Bibliography

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    Related articles in PubMed

    1. [Sleep deprivation aggravates cognitive impairment in septic rats by activating neuronal glycolytic isoenzyme PFKFB3]. Liu Y, et al. Zhonghua Wei Zhong Bing Ji Jiu Yi Xue, 2023 Mar. PMID 36916342
    2. Silencing of PFKFB3 protects podocytes against high glucose‑induced injury by inducing autophagy. Zhu Z, et al. Mol Med Rep, 2021 Nov. PMID 34490476, Free PMC Article
    3. Excitotoxic stimulus stabilizes PFKFB3 causing pentose-phosphate pathway to glycolysis switch and neurodegeneration. Rodriguez-Rodriguez P, et al. Cell Death Differ, 2012 Oct. PMID 22421967, Free PMC Article
    4. Characterization of a new liver- and kidney-specific pfkfb3 isozyme that is downregulated by cell proliferation and dedifferentiation. Duran J, et al. Biochem Biophys Res Commun, 2008 Mar 21. PMID 18191036
    5. 6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase expression in rat brain during development. Goren N, et al. Brain Res Mol Brain Res, 2000 Jan 10. PMID 10648897

    See all (24) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. [Sleep deprivation aggravates cognitive impairment in septic rats by activating neuronal glycolytic isoenzyme PFKFB3].
      Title: [Sleep deprivation aggravates cognitive impairment in septic rats by activating neuronal glycolytic isoenzyme PFKFB3].
    2. Silencing of PFKFB3 protects podocytes against high glucoseinduced injury by inducing autophagy.
      Title: Silencing of PFKFB3 protects podocytes against high glucose‑induced injury by inducing autophagy.
    3. kappa-opioid receptor stimulation alleviates rat vascular smooth muscle cell calcification via PFKFB3-lactate signaling.
      Title: κ-opioid receptor stimulation alleviates rat vascular smooth muscle cell calcification via PFKFB3-lactate signaling.
    4. 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase Suppresses Neuronal Apoptosis by Increasing Glycolysis and ""cyclin-dependent kinase 1-Mediated Phosphorylation of p27 After Traumatic Spinal Cord Injury in Rats.
      Title: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase Suppresses Neuronal Apoptosis by Increasing Glycolysis and "cyclin-dependent kinase 1-Mediated Phosphorylation of p27 After Traumatic Spinal Cord Injury in Rats.
    5. Overexpression of the miR-26a or si-PFKFB3 provides an elevation in cell proliferation. Our study suggests that the miR-26a RNA alleviates lower extremity IRI-induced VEC injury in rats through the activation of the AMPK(AMPK alpha1) pathway by inhibiting PFKFB3.
      Title: Effect of microRNA-26a on vascular endothelial cell injury caused by lower extremity ischemia-reperfusion injury through the AMPK pathway by targeting PFKFB3.
    6. Increased PFKFB3 expression, but not increased PFKFB3 Ser461 phosphorylation, plays a role in increasing glycolysis in mitogen-stimulated thymocytes.
      Title: Role of Akt/PKB and PFKFB isoenzymes in the control of glycolysis, cell proliferation and protein synthesis in mitogen-stimulated thymocytes.
    7. astrocyte proliferation can be effectively suppressed by down-regulation of PFKFB3 through control of glycolytic flux, which is downstream of Cdh1
      Title: PFKFB3-mediated glycolysis is involved in reactive astrocyte proliferation after oxygen-glucose deprivation/reperfusion and is regulated by Cdh1.
    8. The PFK2 expression along with LDH-4 were observed to be increased ~2-fold (P < 0.001) in 0.5 mM ammonia treated brain slices.
      Title: Moderate grade hyperammonemia activates lactate dehydrogenase-4 and 6-phosphofructo-2-kinase to support increased lactate turnover in the brain slices.
    9. The expression of PFKFB3 lacking the APC(Cdh1)-targeting KEN motif, promoted accumulation of PFKFB3 and its release from the nucleus to the cytosol through an excess Cdh1-inhibitable process.
      Title: Excitotoxic stimulus stabilizes PFKFB3 causing pentose-phosphate pathway to glycolysis switch and neurodegeneration.
    10. The maximum expression of Pfkfb3 was detected before puberty, while that of Pfkfb4 was observed in adulthood during rat sperm maturation.
      Title: Switches in 6-phosphofructo-2-kinase isoenzyme expression during rat sperm maturation.
    Submit: New GeneRIF Correction See all GeneRIFs (13)

    Pathways from PubChem

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    General gene information

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    Markers

    Homology

    Gene Ontology Provided by RGD

    Function Evidence Code Pubs
    enables 6-phosphofructo-2-kinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 6-phosphofructo-2-kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables 6-phosphofructo-2-kinase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables 6-phosphofructo-2-kinase activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables fructose-2,6-bisphosphate 2-phosphatase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables fructose-2,6-bisphosphate 2-phosphatase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables fructose-2,6-bisphosphate 2-phosphatase activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    enables fructose-2,6-bisphosphate 2-phosphatase activity TAS
    Traceable Author Statement
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in carbohydrate phosphorylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in fructose 2,6-bisphosphate metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in fructose 2,6-bisphosphate metabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in fructose 2,6-bisphosphate metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in fructose metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of glycolytic process TAS
    Traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
    Names
    6PF-2-K/Fru-2,6-P2ASE brain-type isozyme
    6PF-2-K/Fru-2,6-P2ase 3
    PFK/FBPase 3
    RB2K
    NP_476476.1
    XP_006254254.1
    XP_006254255.1
    XP_006254258.1
    XP_006254259.1
    XP_006254260.1
    XP_017455933.1
    XP_063132102.1
    XP_063132103.1
    XP_063132104.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_057135.1 → NP_476476.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3

      See identical proteins and their annotated locations for NP_476476.1

      Status: PROVISIONAL

      Source sequence(s)
      D87240
      UniProtKB/Swiss-Prot
      O35096, O35552, O35553, O35554, O35555, O35556, O35557, Q9QWQ5, Q9QWQ6
      UniProtKB/TrEMBL
      A6JLS9, A6JLT1
      Related
      ENSRNOP00000062965.1, ENSRNOT00000068354.4
      Conserved Domains (2) summary
      pfam00300
      Location:249 → 435
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      pfam01591
      Location:26 → 246
      6PF2K; 6-phosphofructo-2-kinase

    RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCr8

    Genomic

    1. NC_086035.1 Reference GRCr8

      Range
      71893320..71974526
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_017600444.3 → XP_017455933.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X9

      UniProtKB/TrEMBL
      A6JLT1

    2. XM_063276032.1 → XP_063132102.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X3

      UniProtKB/TrEMBL
      A0A8I6AUG9

    3. XM_063276034.1 → XP_063132104.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X8

    4. XM_063276033.1 → XP_063132103.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X7

    5. XM_006254193.4 → XP_006254255.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X2

      See identical proteins and their annotated locations for XP_006254255.1

      UniProtKB/TrEMBL
      A6JLS7, A6JLT1
      Related
      ENSRNOP00000025700.4
      Conserved Domains (2) summary
      pfam00300
      Location:249 → 435
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      pfam01591
      Location:26 → 246
      6PF2K; 6-phosphofructo-2-kinase

    6. XM_006254192.4 → XP_006254254.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X1

      See identical proteins and their annotated locations for XP_006254254.1

      UniProtKB/TrEMBL
      A6JLS4, A6JLT1
      Related
      ENSRNOP00000040928.4
      Conserved Domains (2) summary
      pfam00300
      Location:249 → 435
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      pfam01591
      Location:26 → 246
      6PF2K; 6-phosphofructo-2-kinase

    7. XM_006254198.4 → XP_006254260.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X6

      See identical proteins and their annotated locations for XP_006254260.1

      UniProtKB/TrEMBL
      A6JLS8, A6JLT2
      Conserved Domains (2) summary
      pfam00300
      Location:249 → 435
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      pfam01591
      Location:26 → 246
      6PF2K; 6-phosphofructo-2-kinase

    8. XM_006254197.4 → XP_006254259.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X5

      See identical proteins and their annotated locations for XP_006254259.1

      UniProtKB/TrEMBL
      A6JLS5, A6JLS6
      Related
      ENSRNOP00000096463.1
      Conserved Domains (2) summary
      pfam00300
      Location:249 → 435
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      pfam01591
      Location:26 → 246
      6PF2K; 6-phosphofructo-2-kinase

    9. XM_006254196.4 → XP_006254258.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X4

      See identical proteins and their annotated locations for XP_006254258.1

      UniProtKB/TrEMBL
      A6JLT0, A6JLT2
      Related
      ENSRNOP00000025731.5
      Conserved Domains (2) summary
      pfam00300
      Location:249 → 435
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      pfam01591
      Location:26 → 246
      6PF2K; 6-phosphofructo-2-kinase
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    O35552.1 GenPept UniProtKB/Swiss-Prot:O35552

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