TEM1 Ras family GTPase TEM1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: TEM1
Gene description: Ras family GTPase TEM1
Primary source: FungiDB:YML064C
Locus tag: YML064C
See related: SGD:S000004529; AllianceGenome:SGD:S000004529; VEuPathDB:YML064C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables GTPase activity. Involved in several processes, including establishment of mitotic spindle localization; negative regulation of protein localization to nucleolus; and regulation of exit from mitosis. Located in spindle pole body. Human ortholog(s) of this gene implicated in Smith-McCort dysplasia and cone-rod dystrophy 18. Orthologous to several human genes including RAB26 (RAB26, member RAS oncogene family); RAB28 (RAB28, member RAS oncogene family); and RAB33A (RAB33A, member RAS oncogene family). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See TEM1 in Genome Data Viewer

Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (145139..145876, complement)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Soluble trivalent engagers redirect cytolytic T cell activity toward tumor endothelial marker 1.
Title: Soluble trivalent engagers redirect cytolytic T cell activity toward tumor endothelial marker 1.
Downregulation of the Tem1 GTPase by Amn1 after cytokinesis involves both nuclear import and SCF-mediated degradation.
Title: Downregulation of the Tem1 GTPase by Amn1 after cytokinesis involves both nuclear import and SCF-mediated degradation.
Spindle pole bodies function as signal amplifiers in the Mitotic Exit Network.
Title: Spindle pole bodies function as signal amplifiers in the Mitotic Exit Network.
Tem1 residence at SPBs depends on its nucleotide state and, importantly, asymmetry of the Bub2-Bfa1-Tem1 complex does not promote mitotic exit but rather controls spindle positioning.
Title: Coupling spindle position with mitotic exit in budding yeast: The multifaceted role of the small GTPase Tem1.
asymmetry of the Bub2/Bfa1/Tem1 complex is crucial to control Kar9 distribution and spindle positioning during mitosis.
Title: Asymmetry of the budding yeast Tem1 GTPase at spindle poles is required for spindle positioning but not for mitotic exit.
Bfa1 localization at spindle pole bodies (SPBs) is independent of Tem1; TEM1 neither alters Bfa1 accumulation at daughter-directed SPB in normal cells nor prevents the localization of Bfa1 at both SPBs in cells with misaligned spindles.
Title: Lte1 contributes to Bfa1 localization rather than stimulating nucleotide exchange by Tem1.
there is a GAP-independent surveillance mechanism of Bfa1/Bub2, which, together with the GTP/GDP switch of Tem1, may be required for the genomic stability of cells with misaligned spindles
Title: Different levels of Bfa1/Bub2 GAP activity are required to prevent mitotic exit of budding yeast depending on the type of perturbations.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables GTP binding	IBA Inferred from Biological aspect of Ancestor more info
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTPase activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activity	IDA Inferred from Direct Assay more info	PubMed
enables GTPase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in establishment of mitotic spindle localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic spindle orientation checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of protein localization to nucleolus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of exit from mitosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of mitotic division septum assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of signaling	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of protein localization to mitotic spindle pole body	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in spindle pole body	IBA Inferred from Biological aspect of Ancestor more info
located_in spindle pole body	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Ras family GTPase TEM1

NP_013647.1

GTPase involved in initiation of Mitotic Exit Network (MEN); GTP-binding protein of the Ras superfamily; accumulates at daughter spindle pole body and activates MEN kinase cascade; controls actomyosin and septin dynamics during cytokinesis