AFG3 AAA family ATPase AFG3 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: AFG3
Gene description: AAA family ATPase AFG3
Primary source: FungiDB:YER017C
Locus tag: YER017C
See related: SGD:S000000819; AllianceGenome:SGD:S000000819; VEuPathDB:YER017C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: YTA10
Summary: Enables ATP hydrolysis activity and metallopeptidase activity. Involved in cellular protein-containing complex assembly; protein import into mitochondrial matrix; and signal peptide processing. Located in mitochondrial inner boundary membrane. Part of m-AAA complex. Colocalizes with fungal-type vacuole membrane. Used to study hereditary spastic paraplegia and spinocerebellar ataxia type 28. Human ortholog(s) of this gene implicated in optic atrophy 12; spastic ataxia; spastic ataxia 5; and spinocerebellar ataxia type 28. Orthologous to human AFG3L2 (AFG3 like matrix AAA peptidase subunit 2). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See AFG3 in Genome Data Viewer

Location:: chromosome: V

Exon count:: 1

Sequence:: Chromosome: V; NC_001137.3 (189503..191788, complement)

Chromosome V - NC_001137.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001137.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

replacement of the Yta10 TM2 domain abolishes membrane dislocation for only a subset of substrates, whereas replacement of the Yta12 TM2 domain impairs membrane dislocation for all tested substrates, suggesting different roles of the TM domains in each m-AAA protease subunit
Title: Molecular insights into the m-AAA protease-mediated dislocation of transmembrane helices in the mitochondrial inner membrane.
Observations show a role for the mitochondrial protease Afg3 in modulating cytoplasmic mRNA translation and ER stress resistance, and suggest Afg3 modulates longevity in yeast by a mechanism similar to cytoplasmic ribosomal large subunit proteins.
Title: Stress profiling of longevity mutants identifies Afg3 as a mitochondrial determinant of cytoplasmic mRNA translation and aging.
Inactivation of either of the mitochondrial AAA proteases, Yme1 or Yta10/12, allows fermentative growth of hsc82 or hsp82 strains at 37 degrees C.
Title: Hsp90 and mitochondrial proteases Yme1 and Yta10/12 participate in ATP synthase assembly in Saccharomyces cerevisiae.
Results describe the localizations of the m-AAA protease, its subunits Yta10 and Yta12, and its substrate cytochrome c peroxidase (Ccp1) within yeast mitochondria.
Title: The m-AAA protease processes cytochrome c peroxidase preferentially at the inner boundary membrane of mitochondria.
transmembrane segments of m-AAA protease subunits have a direct role in the dislocation of membrane-embedded substrates
Title: Membrane protein turnover by the m-AAA protease in mitochondria depends on the transmembrane domains of its subunits.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables ATP-dependent peptidase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables metalloendopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables metalloendopeptidase activity	IEA Inferred from Electronic Annotation more info
enables metallopeptidase activity	IEA Inferred from Electronic Annotation more info
enables metallopeptidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in mitochondrial protein processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein import into mitochondrial matrix	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein-containing complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteolysis	IEA Inferred from Electronic Annotation more info
involved_in signal peptide processing	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of m-AAA complex	IBA Inferred from Biological aspect of Ancestor more info
part_of m-AAA complex	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrial inner boundary membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial inner membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial inner membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: AAA family ATPase AFG3

NP_010933.1

Mitochondrial inner membrane m-AAA protease component; mediates degradation of misfolded or unassembled proteins; also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging; expression of human homolog AFG3L2 can complement yeast yta12 afg3 double mutant

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001137.3 Reference assembly

Range

189503..191788 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178908.1 → NP_010933.1 TPA: AAA family ATPase AFG3 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010933.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DLR5, P39925

UniProtKB/TrEMBL

A0A6A5PWC2, B3LS29, G2WCP9

Conserved Domains (2) summary

TIGR01241
Location:223 → 730

FtsH_fam; ATP-dependent metalloprotease FtsH

pfam06480
Location:124 → 215

FtsH_ext; FtsH Extracellular