ORM2 sphingolipid homeostasis protein ORM2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: ORM2
Gene description: sphingolipid homeostasis protein ORM2
Primary source: FungiDB:YLR350W
Locus tag: YLR350W
See related: SGD:S000004342; AllianceGenome:SGD:S000004342; VEuPathDB:YLR350W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Involved in negative regulation of sphingolipid biosynthetic process and response to unfolded protein. Located in endoplasmic reticulum. Part of SPOTS complex. Used to study Loeys-Dietz syndrome and asthma. Human ortholog(s) of this gene implicated in asthma. Orthologous to human ORMDL1 (ORMDL sphingolipid biosynthesis regulator 1); ORMDL2 (ORMDL sphingolipid biosynthesis regulator 2); and ORMDL3 (ORMDL sphingolipid biosynthesis regulator 3). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See ORM2 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (828730..829380)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Deletion of ORM2 Causes Oleic Acid-Induced Growth Defects in Saccharomyces cerevisiae.
Title: Deletion of ORM2 Causes Oleic Acid-Induced Growth Defects in Saccharomyces cerevisiae.
Orm2 degradation is initiated by phosphorylation, which triggers its ER export. Once on Golgi and endosomes, Orm2 is poly-ubiquitinated by the membrane-embedded Dsc ubiquitin ligase complex. Cdc48/VCP then extracts ubiquitinated Orm2 from membranes, which is tightly coupled to the proteasomal degradation of Orm2.
Title: Endosome and Golgi-associated degradation (EGAD) of membrane proteins regulates sphingolipid metabolism.
Data indicate that Orm1 and Orm2 proteins respond to feedback signals from the sphingolipid synthesis pathway.
Title: Orm proteins integrate multiple signals to maintain sphingolipid homeostasis.
Npr1-phosphorylated Orm1 and Orm2 stimulate de novo synthesis of complex sphingolipids downstream of serine palmitoyltransferase.
Title: TORC1-regulated protein kinase Npr1 phosphorylates Orm to stimulate complex sphingolipid synthesis.
Orm1 and Orm2 regulated the synthesis of sphingolipid.
Title: Regulation of sphingolipid synthesis through Orm1 and Orm2 in yeast.
Data show a mechanism through which the conserved Pkh-Ypk kinase cascade and Cdc55-PP2A facilitate rapid, transient sphingolipid production in response to heat stress through Orm protein phosphoregulation.
Title: Orm protein phosphoregulation mediates transient sphingolipid biosynthesis response to heat stress via the Pkh-Ypk and Cdc55-PP2A pathways.
Physical interaction between Orm1 and Orm2 and serine palmitoyltransferase, responsible for the first committed step in sphingolipid synthesis, further supports a role for the Orm proteins in regulating sphingolipid synthesis.
Title: Orm1 and Orm2 are conserved endoplasmic reticulum membrane proteins regulating lipid homeostasis and protein quality control.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables molecular_function	ND No biological Data available more info

Process	Evidence Code	Pubs
involved_in ceramide metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in intracellular sphingolipid homeostasis	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of sphingolipid biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of sphingolipid biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to unfolded protein	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sphingolipid biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of SPOTS complex	IBA Inferred from Biological aspect of Ancestor more info
part_of SPOTS complex	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	HDA more info	PubMed
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: sphingolipid homeostasis protein ORM2

NP_013454.1

Protein that mediates sphingolipid homeostasis; evolutionarily conserved, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress; ORM2 has a paralog, ORM1, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

828730..829380

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182239.1 → NP_013454.1 TPA: sphingolipid homeostasis protein ORM2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013454.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VYY8, Q06144

UniProtKB/TrEMBL

A0A6L0ZQC3, A7A1M8, B3RHL9, B5VNP0, C7GRX5, C8ZDV8, G2WJG8, N1NYN7

Conserved Domains (1) summary

COG5081
Location:37 → 216

COG5081; Predicted membrane protein [Function unknown]