Apob apolipoprotein B [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Apobprovided by MGI
Official Full Name: apolipoprotein Bprovided by MGI
Primary source: MGI:MGI:88052
See related: Ensembl:ENSMUSG00000020609 AllianceGenome:MGI:88052
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: apob-48; apob-100; Apo B-100
Summary: This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100. Unlike the apoB-48 and apoB-100 structural equivalents in human, which are synthesized exclusively in the gut and liver, respectively, the mouse apoB-48 isoform is also found in mouse liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2179 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. [provided by RefSeq, Jul 2008]
Expression: Biased expression in placenta adult (RPKM 182.2), liver adult (RPKM 82.4) and 5 other tissues See more
Orthologs: human all
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Genomic context

Location:: 12 A1.1; 12 3.53 cM

Exon count:: 30

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	12	NC_000078.7 (8027629..8066839)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	12	NC_000078.6 (7977629..8016839)

Chromosome 12 - NC_000078.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Conditional hepatocyte ablation of PDIA1 uncovers indispensable roles in both APOB and MTTP folding to support VLDL secretion.
Title: Conditional hepatocyte ablation of PDIA1 uncovers indispensable roles in both APOB and MTTP folding to support VLDL secretion.
PCSK7: A novel regulator of apolipoprotein B and a potential target against non-alcoholic fatty liver disease.
Title: PCSK7: A novel regulator of apolipoprotein B and a potential target against non-alcoholic fatty liver disease.
Menaquinone 4 increases plasma lipid levels in hypercholesterolemic mice.
Title: Menaquinone 4 increases plasma lipid levels in hypercholesterolemic mice.
Pathogenic Autoimmunity in Atherosclerosis Evolves From Initially Protective Apolipoprotein B100-Reactive CD4(+) T-Regulatory Cells.
Title: Pathogenic Autoimmunity in Atherosclerosis Evolves From Initially Protective Apolipoprotein B(100)-Reactive CD4(+) T-Regulatory Cells.
Sex-differences in echocardiographic assessment of aortic valve in young adult LDLr(-/-)/ApoB(100/100)/IGF-II(+/-) mice.
Title: Sex-differences in echocardiographic assessment of aortic valve in young adult LDLr(-/-)/ApoB(100/100)/IGF-II(+/-) mice.
Major histocompatibility complex class II molecule-restricted peptide epitopes of ApoB function as Treg epitopes.
Title: Regulatory CD4(+) T Cells Recognize Major Histocompatibility Complex Class II Molecule-Restricted Peptide Epitopes of Apolipoprotein B.
Type 2 diabetic, hyperlipidemic LDLr(-/-)ApoB(100/100) mice show increased calcific aortic valve disease.
Title: Increased Calcific Aortic Valve Disease in response to a diabetogenic, procalcific diet in the LDLr(-/-)ApoB(100/100) mouse model.
This study demonstrates that prevention of renal apoB accumulation is a mechanism by which TGF-beta inhibition is nephroprotective.
Title: Prevention of renal apoB retention is protective against diabetic nephropathy: role of TGF-β inhibition.
Atorvastatin therapy did not show cholesterol-independent effects on inflammation in atherosclerotic lesions in Ldlr(-/-)Apob(100/100) mice, whereas a cholesterol-lowering diet intervention was effective.
Title: Effects of atorvastatin and diet interventions on atherosclerotic plaque inflammation and [(18)F]FDG uptake in Ldlr(-/-)Apob(100/100) mice.
A peptide fragment of apoB-100 altered the immune-dominant epitope of CD8+ T cells and reduced atherosclerosis.
Title: Identification of apoB-100 Peptide-Specific CD8+ T Cells in Atherosclerosis.

Submit: New GeneRIF Correction See all GeneRIFs (64)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (11) 1 citation
Endonuclease-mediated (4)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:236804

Apob (e-PCR), detects polymorphism
- UniSTS:141110

PMC316377P5 (e-PCR)
- UniSTS:272972

Apob (e-PCR), detects polymorphism
- UniSTS:259467

AI315052 (e-PCR)
- UniSTS:160183

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables cholesterol transfer activity	IBA Inferred from Biological aspect of Ancestor more info
enables cholesterol transfer activity	ISO Inferred from Sequence Orthology more info
enables heparin binding	ISO Inferred from Sequence Orthology more info
enables lipase binding	ISO Inferred from Sequence Orthology more info
enables lipid transporter activity	IEA Inferred from Electronic Annotation more info
enables low-density lipoprotein particle receptor binding	IBA Inferred from Biological aspect of Ancestor more info
enables low-density lipoprotein particle receptor binding	ISO Inferred from Sequence Orthology more info
enables phospholipid binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within artery morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within cholesterol efflux	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cholesterol homeostasis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within cholesterol homeostasis	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within cholesterol homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cholesterol homeostasis	ISO Inferred from Sequence Orthology more info
involved_in cholesterol metabolic process	ISO Inferred from Sequence Orthology more info
involved_in cholesterol transport	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within cholesterol transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cholesterol transport	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within establishment of localization in cell	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within fertilization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within flagellated sperm motility	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within in utero embryonic development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within lipid catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within lipid metabolic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within lipid metabolic process	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within lipid metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within lipid transport	IEA Inferred from Electronic Annotation more info
involved_in lipid transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within lipoprotein biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within lipoprotein catabolic process	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within lipoprotein metabolic process	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within lipoprotein metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lipoprotein transport	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within lipoprotein transport	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within lipoprotein transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in low-density lipoprotein particle clearance	ISO Inferred from Sequence Orthology more info
involved_in low-density lipoprotein particle remodeling	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within nervous system development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cholesterol storage	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of lipid storage	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of macrophage derived foam cell differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within post-embryonic development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of cholesterol biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to carbohydrate	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within steroid metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within triglyceride catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in triglyceride catabolic process	ISO Inferred from Sequence Orthology more info
involved_in triglyceride mobilization	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within triglyceride mobilization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of chylomicron	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum exit site	ISO Inferred from Sequence Orthology more info
located_in extracellular region	IEA Inferred from Electronic Annotation more info
located_in extracellular region	TAS Traceable Author Statement more info
located_in extracellular space	ISO Inferred from Sequence Orthology more info
part_of high-density lipoprotein particle	ISO Inferred from Sequence Orthology more info
part_of intermediate-density lipoprotein particle	ISO Inferred from Sequence Orthology more info
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
located_in lipid droplet	IEA Inferred from Electronic Annotation more info
part_of low-density lipoprotein particle	IBA Inferred from Biological aspect of Ancestor more info
part_of low-density lipoprotein particle	ISO Inferred from Sequence Orthology more info
part_of mature chylomicron	IBA Inferred from Biological aspect of Ancestor more info
part_of mature chylomicron	ISO Inferred from Sequence Orthology more info
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info	PubMed
part_of very-low-density lipoprotein particle	IBA Inferred from Biological aspect of Ancestor more info
part_of very-low-density lipoprotein particle	ISO Inferred from Sequence Orthology more info
located_in vesicle lumen	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: apolipoprotein B-100

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_009693.2 → NP_033823.2 apolipoprotein B-100 precursor

See identical proteins and their annotated locations for NP_033823.2

Status: REVIEWED

Source sequence(s)

AC163900, AI324120, AK147540

Consensus CDS

CCDS49022.1

UniProtKB/Swiss-Prot

E9Q414, Q3UH74, Q497D8, Q61314, Q61318, Q8CGG8

UniProtKB/TrEMBL

E9Q1Y3

Related

ENSMUSP00000036044.7, ENSMUST00000037811.13

Conserved Domains (4) summary

pfam01347
Location:46 → 597

Vitellogenin_N; Lipoprotein amino terminal region

pfam06448
Location:961 → 1062

DUF1081; Domain of Unknown Function (DUF1081)

pfam09172
Location:632 → 944

DUF1943; Domain of unknown function (DUF1943)

pfam12491
Location:4434 → 4490

ApoB100_C; Apolipoprotein B100 C terminal

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000078.7 Reference GRCm39 C57BL/6J

Range

8027629..8066839

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006515078.3 → XP_006515141.1 apolipoprotein B-100 isoform X1

UniProtKB/TrEMBL

E9Q1Y3

Conserved Domains (4) summary

pfam06448
Location:960 → 1062

DUF1081; Domain of Unknown Function (DUF1081)

pfam09172
Location:632 → 937

DUF1943; Domain of unknown function (DUF1943)

pfam06160
Location:2099 → 2486

EzrA; Septation ring formation regulator, EzrA

cl26969
Location:46 → 597

Vitellogenin_N; Lipoprotein amino terminal region