Tex14 testis expressed gene 14 intercellular bridge forming factor [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Tex14provided by MGI
Official Full Name: testis expressed gene 14 intercellular bridge forming factorprovided by MGI
Primary source: MGI:MGI:1933227
See related: Ensembl:ENSMUSG00000010342 AllianceGenome:MGI:1933227
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Summary: Predicted to enable protein kinase binding activity. Involved in several processes, including intercellular bridge organization; mitotic spindle assembly checkpoint signaling; and nuclear division. Acts upstream of or within cellular response to leukemia inhibitory factor; negative regulation of cytokinesis; and negative regulation of protein binding activity. Located in intercellular bridge; kinetochore; and midbody. Is expressed in germ cell of ovary; germ cell of testis; ovary; and primordial germ cell of gonad. Human ortholog(s) of this gene implicated in spermatogenic failure 23. Orthologous to human TEX14 (testis expressed 14, intercellular bridge forming factor). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in testis adult (RPKM 6.6) and placenta adult (RPKM 0.2) See more
Orthologs: human all
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Genomic context

Location:: 11 C; 11 52.09 cM

Exon count:: 35

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (87295839..87446649)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (87405013..87555823)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Altered germline cyst formation and oogenesis in Tex14 mutant mice.
Title: Altered germline cyst formation and oogenesis in Tex14 mutant mice.
Data indicate that testis-expressed gene 14 (TEX14) is expressed in germ cells.
Title: Structural and biochemical insights into the role of testis-expressed gene 14 (TEX14) in forming the stable intercellular bridges of germ cells.
RBM44 interacts with itself and TEX14 using yeast two-hybrid, mammalian two-hybrid, and immunoprecipitation.
Title: Identification and characterization of RBM44 as a novel intercellular bridge protein.
TEX14 prevents the completion of cytokinesis by altering the destiny of CEP55 from a nidus for abscission to an integral component of the intercellular bridge.
Title: TEX14 interacts with CEP55 to block cell abscission.
Despite the absence of embryonic intercellular bridges in the Tex14-null mice, male mice initiate spermatogenesis, and female mice are fertile. But fewer oocytes were present in Tex14-null neonatal ovaries.
Title: Mouse TEX14 is required for embryonic germ cell intercellular bridges but not female fertility.
Predicted to be localized to the nucleus, suggesting a key role in regulating gene expression during mammalian spermatogenesis.
Title: Sequence and expression of testis-expressed gene 14 (Tex14): a gene encoding a protein kinase preferentially expressed during spermatogenesis.
TEX14 is required for intercellular bridges in vertebrate germ cells, and these studies provide evidence that the intercellular bridge is essential for spermatogenesis and fertility
Title: TEX14 is essential for intercellular bridges and fertility in male mice.
Tex14 is expressed throughout spermatogenesis
Title: Differential expression of sex-linked and autosomal germ-cell-specific genes during spermatogenesis in the mouse.
Tex14 is specifically expressed in testis in a cDNA subtraction screen
Title: An abundance of X-linked genes expressed in spermatogonia.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (1) 1 citation
Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

C85585 (e-PCR)
- UniSTS:180285

D11Mit323 (e-PCR), detects polymorphism
- UniSTS:126536

G65216 (e-PCR)
- UniSTS:225885

G65790 (e-PCR)
- UniSTS:225926

D11Mit56 (e-PCR), detects polymorphism
- UniSTS:126602

D11Mit158 (e-PCR), detects polymorphism
- UniSTS:126350

Rnu1a1 (e-PCR), detects polymorphism
- UniSTS:144352

D11Fpm103 (e-PCR)
- UniSTS:510460

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein kinase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in attachment of spindle microtubules to kinetochore	IBA Inferred from Biological aspect of Ancestor more info
involved_in attachment of spindle microtubules to kinetochore	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cell cycle	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cell division	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cellular response to leukemia inhibitory factor	IEP Inferred from Expression Pattern more info	PubMed
involved_in intercellular bridge organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in intercellular bridge organization	IDA Inferred from Direct Assay more info	PubMed
involved_in male meiotic nuclear division	IBA Inferred from Biological aspect of Ancestor more info
involved_in male meiotic nuclear division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic sister chromatid separation	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic sister chromatid separation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic spindle assembly checkpoint signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic spindle assembly checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of cytokinesis	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of protein binding	IDA Inferred from Direct Assay more info	PubMed
involved_in protein phosphorylation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in chromosome, centromeric region	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in intercellular bridge	IBA Inferred from Biological aspect of Ancestor more info
located_in intercellular bridge	IDA Inferred from Direct Assay more info	PubMed
is_active_in kinetochore	IBA Inferred from Biological aspect of Ancestor more info
located_in kinetochore	IDA Inferred from Direct Assay more info	PubMed
is_active_in midbody	IBA Inferred from Biological aspect of Ancestor more info
located_in midbody	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: inactive serine/threonine-protein kinase TEX14

Names: testis-expressed protein 14; testis-expressed sequence 14 protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001199293.1 → NP_001186222.1 inactive serine/threonine-protein kinase TEX14 isoform a

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform a).

Source sequence(s)

AL669902, BK000967

UniProtKB/Swiss-Prot

Q7M6U3

Conserved Domains (5) summary

COG0515
Location:269 → 632

SPS1; Serine/threonine protein kinase [Signal transduction mechanisms]

cd00204
Location:39 → 137

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam12796
Location:39 → 131

Ank_2; Ankyrin repeats (3 copies)

sd00045
Location:67 → 98

ANK; ANK repeat [structural motif]

cl21453
Location:282 → 519

PKc_like; Protein Kinases, catalytic domain
NM_031386.2 → NP_113563.2 inactive serine/threonine-protein kinase TEX14 isoform b

See identical proteins and their annotated locations for NP_113563.2

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses a different first exon which results in the use of a downstream start codon, compared to variant 1. The resulting protein (isoform b) has a shorter N-terminus when it is compared to isoform a.

Source sequence(s)

AL669902, BK000966

Consensus CDS

CCDS48877.1

UniProtKB/Swiss-Prot

B2KGL0, Q3UT36, Q5NC10, Q5NC11, Q7M6U3, Q8CGK1, Q8CGK2, Q99MV8

Related

ENSMUSP00000054444.6, ENSMUST00000060835.12

Conserved Domains (5) summary

COG0515
Location:257 → 620

SPS1; Serine/threonine protein kinase [Signal transduction mechanisms]

cd00204
Location:27 → 125

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam12796
Location:27 → 119

Ank_2; Ankyrin repeats (3 copies)

sd00045
Location:55 → 86

ANK; ANK repeat [structural motif]

cl21453
Location:270 → 507

PKc_like; Protein Kinases, catalytic domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000077.7 Reference GRCm39 C57BL/6J

Range

87295839..87446649

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011249327.3 → XP_011247629.1 inactive serine/threonine-protein kinase TEX14 isoform X1

See identical proteins and their annotated locations for XP_011247629.1

Conserved Domains (5) summary

PTZ00322
Location:72 → 179

PTZ00322; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase; Provisional

COG5214
Location:458 → 658

POL12; DNA polymerase alpha-primase complex, polymerase-associated subunit B [DNA replication, recombination, and repair]

sd00045
Location:55 → 86

ANK; ANK repeat [structural motif]

pfam12796
Location:27 → 119

Ank_2; Ankyrin repeats (3 copies)

cl21453
Location:270 → 507

PKc_like; Protein Kinases, catalytic domain
XM_017314856.2 → XP_017170345.1 inactive serine/threonine-protein kinase TEX14 isoform X3

Conserved Domains (5) summary

PTZ00322
Location:72 → 179

PTZ00322; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase; Provisional

COG5214
Location:458 → 658

POL12; DNA polymerase alpha-primase complex, polymerase-associated subunit B [DNA replication, recombination, and repair]

sd00045
Location:55 → 86

ANK; ANK repeat [structural motif]

pfam12796
Location:27 → 119

Ank_2; Ankyrin repeats (3 copies)

cl21453
Location:270 → 507

PKc_like; Protein Kinases, catalytic domain
XM_017314855.2 → XP_017170344.1 inactive serine/threonine-protein kinase TEX14 isoform X2

Conserved Domains (5) summary

PTZ00322
Location:72 → 179

PTZ00322; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase; Provisional

COG5214
Location:458 → 658

POL12; DNA polymerase alpha-primase complex, polymerase-associated subunit B [DNA replication, recombination, and repair]

sd00045
Location:55 → 86

ANK; ANK repeat [structural motif]

pfam12796
Location:27 → 119

Ank_2; Ankyrin repeats (3 copies)

cl21453
Location:270 → 507

PKc_like; Protein Kinases, catalytic domain
XM_017314857.2 → XP_017170346.1 inactive serine/threonine-protein kinase TEX14 isoform X4

Conserved Domains (5) summary

PTZ00322
Location:72 → 179

PTZ00322; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase; Provisional

COG5214
Location:458 → 658

POL12; DNA polymerase alpha-primase complex, polymerase-associated subunit B [DNA replication, recombination, and repair]

sd00045
Location:55 → 86

ANK; ANK repeat [structural motif]

pfam12796
Location:27 → 119

Ank_2; Ankyrin repeats (3 copies)

cl21453
Location:270 → 507

PKc_like; Protein Kinases, catalytic domain
XM_017314858.2 → XP_017170347.1 inactive serine/threonine-protein kinase TEX14 isoform X7

Conserved Domains (5) summary

PTZ00322
Location:72 → 179

PTZ00322; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase; Provisional

COG5214
Location:458 → 658

POL12; DNA polymerase alpha-primase complex, polymerase-associated subunit B [DNA replication, recombination, and repair]

sd00045
Location:55 → 86

ANK; ANK repeat [structural motif]

pfam12796
Location:27 → 119

Ank_2; Ankyrin repeats (3 copies)

cl21453
Location:270 → 507

PKc_like; Protein Kinases, catalytic domain
XM_011249328.3 → XP_011247630.1 inactive serine/threonine-protein kinase TEX14 isoform X5

See identical proteins and their annotated locations for XP_011247630.1

Conserved Domains (2) summary

COG5214
Location:240 → 440

POL12; DNA polymerase alpha-primase complex, polymerase-associated subunit B [DNA replication, recombination, and repair]

cl21453
Location:52 → 289

PKc_like; Protein Kinases, catalytic domain
XM_011249329.1 → XP_011247631.1 inactive serine/threonine-protein kinase TEX14 isoform X6

See identical proteins and their annotated locations for XP_011247631.1

Conserved Domains (2) summary

COG5214
Location:169 → 369

POL12; DNA polymerase alpha-primase complex, polymerase-associated subunit B [DNA replication, recombination, and repair]

cl21453
Location:7 → 218

PKc_like; Protein Kinases, catalytic domain