Prkca protein kinase C, alpha [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Prkcaprovided by MGI
Official Full Name: protein kinase C, alphaprovided by MGI
Primary source: MGI:MGI:97595
See related: Ensembl:ENSMUSG00000050965 AllianceGenome:MGI:97595
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Pkca
Summary: Enables calcium-dependent protein kinase C activity and integrin binding activity. Involved in several processes, including modulation of chemical synaptic transmission; positive regulation of bone resorption; and positive regulation of dense core granule biogenesis. Acts upstream of or within several processes, including induction of positive chemotaxis; negative regulation of signal transduction; and regulation of protein phosphorylation. Located in several cellular components, including calyx of Held; intercalated disc; and photoreceptor outer segment. Part of alphav-beta3 integrin-PKCalpha complex. Is active in presynaptic cytosol. Is expressed in several structures, including alimentary system; brain; early embryo; genitourinary system; and sensory organ. Human ortholog(s) of this gene implicated in dilated cardiomyopathy; high grade glioma (multiple); large cell carcinoma; and reproductive organ cancer (multiple). Orthologous to human PRKCA (protein kinase C alpha). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in frontal lobe adult (RPKM 19.9), cortex adult (RPKM 11.1) and 22 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 E1; 11 70.8 cM

Exon count:: 18

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (107824213..108237360, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (107933387..108343888, complement)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Cav-1 regulates the bile salt export pump on the canalicular membrane of hepatocytes by PKCalpha-associated signalling under cholesterol stimulation.
Title: Cav-1 regulates the bile salt export pump on the canalicular membrane of hepatocytes by PKCα-associated signalling under cholesterol stimulation.
Inhibition of protein kinase C-alpha and activation of ezrin by Lactobacillus acidophilus restore Na[+]/H[+] exchange activity and fluid absorption in db/db mice.
Title: Inhibition of protein kinase C-α and activation of ezrin by Lactobacillus acidophilus restore Na(+)/H(+) exchange activity and fluid absorption in db/db mice.
Tectorigenin targets PKACalpha to promote GLUT4 expression in skeletal muscle and improve insulin resistance in vitro and in vivo.
Title: Tectorigenin targets PKACα to promote GLUT4 expression in skeletal muscle and improve insulin resistance in vitro and in vivo.
Extracellular Histone-Induced Protein Kinase C Alpha Activation and Troponin Phosphorylation Is a Potential Mechanism of Cardiac Contractility Depression in Sepsis.
Title: Extracellular Histone-Induced Protein Kinase C Alpha Activation and Troponin Phosphorylation Is a Potential Mechanism of Cardiac Contractility Depression in Sepsis.
PRKCA Promotes Mitophagy through the miR-15a-5p/PDK4 Axis to Relieve Sepsis-Induced Acute Lung Injury.
Title: PRKCA Promotes Mitophagy through the miR-15a-5p/PDK4 Axis to Relieve Sepsis-Induced Acute Lung Injury.
Enhanced activity of Alzheimer disease-associated variant of protein kinase Calpha drives cognitive decline in a mouse model.
Title: Enhanced activity of Alzheimer disease-associated variant of protein kinase Cα drives cognitive decline in a mouse model.
Lipid-independent activation of a muscle-specific PKCalpha splicing variant.
Title: Lipid-independent activation of a muscle-specific PKCα splicing variant.
Evidence that PKCalpha inhibition in Dalton's Lymphoma cells augments cell cycle arrest and mitochondrial-dependent apoptosis.
Title: Evidence that PKCα inhibition in Dalton's Lymphoma cells augments cell cycle arrest and mitochondrial-dependent apoptosis.
All-trans retinoic acid and protein kinase C alpha/beta1 inhibitor combined treatment targets cancer stem cells and impairs breast tumor progression.
Title: All-trans retinoic acid and protein kinase C α/β1 inhibitor combined treatment targets cancer stem cells and impairs breast tumor progression.
Extracellular Matrix Stiffness Regulates DNA Methylation by PKCalpha-Dependent Nuclear Transport of DNMT3L.
Title: Extracellular Matrix Stiffness Regulates DNA Methylation by PKCα-Dependent Nuclear Transport of DNMT3L.

Submit: New GeneRIF Correction See all GeneRIFs (215)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (3) 1 citation
Endonuclease-mediated (5)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Prkca (e-PCR), detects polymorphism
- UniSTS:507177

NoName (e-PCR)
- UniSTS:548152

Prkca (e-PCR), detects polymorphism
- UniSTS:144191

Prkca (e-PCR), detects polymorphism
- UniSTS:144192

Prkca (e-PCR), detects polymorphism
- UniSTS:144193

Prkca (e-PCR), detects polymorphism
- UniSTS:239205

Prkca (e-PCR), detects polymorphism
- UniSTS:239206

Prkca (e-PCR), detects polymorphism
- UniSTS:239207

AI875142 (e-PCR)
- UniSTS:165806

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables calcium,diacylglycerol-dependent serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables calcium,diacylglycerol-dependent serine/threonine kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables calcium,diacylglycerol-dependent serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables diacylglycerol-dependent serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables diacylglycerol-dependent serine/threonine kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables diacylglycerol-dependent serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables histone H3T6 kinase activity	ISO Inferred from Sequence Orthology more info
enables integrin binding	IPI Inferred from Physical Interaction more info	PubMed
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables lipid binding	EXP Inferred from Experiment more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within angiogenesis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within apoptotic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cell adhesion	IEA Inferred from Electronic Annotation more info
acts_upstream_of cell population proliferation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within cellular response to carbohydrate stimulus	IDA Inferred from Direct Assay more info	PubMed
involved_in central nervous system neuron axonogenesis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within chondrocyte differentiation	IDA Inferred from Direct Assay more info	PubMed
involved_in desmosome assembly	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within induction of positive chemotaxis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within intracellular calcium ion homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in intracellular signal transduction	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within intrinsic apoptotic signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in learning or memory	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within muscle cell cellular homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of MAPK cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of anion channel activity	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of cell population proliferation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of glial cell apoptotic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of glucose import	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of insulin receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of protein kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of protein phosphorylation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within negative regulation of protein phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of translation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within neutrophil chemotaxis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within peptidyl-serine autophosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in peptidyl-serine phosphorylation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of ERK1 and ERK2 cascade	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of angiogenesis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of angiotensin-activated signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of blood vessel endothelial cell migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of bone resorption	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cardiac muscle hypertrophy	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell adhesion	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of dense core granule biogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of endothelial cell migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of endothelial cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of exocytosis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of inflammatory response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of lipopolysaccharide-mediated signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of macrophage differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of mitotic cell cycle	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of protein phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of smooth muscle cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of synapse assembly	ISO Inferred from Sequence Orthology more info
involved_in post-translational protein modification	ISO Inferred from Sequence Orthology more info
involved_in presynaptic modulation of chemical synaptic transmission	IDA Inferred from Direct Assay more info	PubMed
involved_in presynaptic modulation of chemical synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of protein phosphorylation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of protein phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of muscle contraction	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of peptidyl-tyrosine phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of platelet aggregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of platelet aggregation	ISO Inferred from Sequence Orthology more info
involved_in regulation of receptor-mediated endocytosis	ISO Inferred from Sequence Orthology more info
involved_in regulation of response to osmotic stress	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of synaptic vesicle exocytosis	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of synaptic vesicle exocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of the force of heart contraction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to estradiol	ISO Inferred from Sequence Orthology more info
involved_in response to ethanol	ISO Inferred from Sequence Orthology more info
involved_in response to interleukin-1	ISO Inferred from Sequence Orthology more info
involved_in response to reactive oxygen species	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within stem cell differentiation	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of alphav-beta3 integrin-PKCalpha complex	IDA Inferred from Direct Assay more info	PubMed
located_in apical part of cell	IDA Inferred from Direct Assay more info	PubMed
located_in axon	IDA Inferred from Direct Assay more info	PubMed
is_active_in calyx of Held	IDA Inferred from Direct Assay more info	PubMed
is_active_in calyx of Held	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in ciliary basal body	ISO Inferred from Sequence Orthology more info
located_in cone photoreceptor outer segment	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	TAS Traceable Author Statement more info
located_in dendrite	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in intercalated disc	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in ooplasm	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in presynapse	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in presynaptic cytosol	IDA Inferred from Direct Assay more info	PubMed
part_of protein-containing complex	IDA Inferred from Direct Assay more info	PubMed
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: protein kinase C alpha type

Names: PKC-A; PKC-alpha; protein kinase c-alpha

NP_035231.2

EC 2.7.11.13

XP_036012317.1

EC 2.7.11.13

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_011101.3 → NP_035231.2 protein kinase C alpha type

See identical proteins and their annotated locations for NP_035231.2

Status: VALIDATED

Source sequence(s)

AK147084, AL645535, BF455952

Consensus CDS

CCDS25573.1

UniProtKB/Swiss-Prot

P20444

UniProtKB/TrEMBL

Q4VA93

Related

ENSMUSP00000062392.5, ENSMUST00000059595.11

Conserved Domains (4) summary

cd04026
Location:159 → 289

C2_PKC_alpha_gamma; C2 domain in Protein Kinase C (PKC) alpha and gamma

cd05615
Location:328 → 668

STKc_cPKC_alpha; Catalytic domain of the Serine/Threonine Kinase, Classical Protein Kinase C alpha

cd20833
Location:35 → 92

C1_cPKC_rpt1; first protein kinase C conserved region 1 (C1 domain) found in the classical (or conventional) protein kinase C (cPKC) family

cd20836
Location:102 → 155

C1_cPKC_rpt2; second protein kinase C conserved region 1 (C1 domain) found in the classical (or conventional) protein kinase C (cPKC) family

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000077.7 Reference GRCm39 C57BL/6J

Range

107824213..108237360 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036156424.1 → XP_036012317.1 protein kinase C alpha type isoform X1

UniProtKB/Swiss-Prot

P20444

Conserved Domains (4) summary

cd04026
Location:159 → 289

C2_PKC_alpha_gamma; C2 domain in Protein Kinase C (PKC) alpha and gamma

cd05615
Location:328 → 684

STKc_cPKC_alpha; Catalytic domain of the Serine/Threonine Kinase, Classical Protein Kinase C alpha

cd20833
Location:35 → 92

C1_cPKC_rpt1; first protein kinase C conserved region 1 (C1 domain) found in the classical (or conventional) protein kinase C (cPKC) family

cd20836
Location:102 → 155

C1_cPKC_rpt2; second protein kinase C conserved region 1 (C1 domain) found in the classical (or conventional) protein kinase C (cPKC) family