Eprs1 glutamyl-prolyl-tRNA synthetase 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Eprs1provided by MGI
Official Full Name: glutamyl-prolyl-tRNA synthetase 1provided by MGI
Primary source: MGI:MGI:97838
See related: Ensembl:ENSMUSG00000026615 AllianceGenome:MGI:97838
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Eprs; Qprs; 2410081F06Rik; 3010002K18Rik
Summary: Enables proline-tRNA ligase activity. Involved in several processes, including cellular response to insulin stimulus; prolyl-tRNA aminoacylation; and regulation of long-chain fatty acid import into cell. Acts upstream of or within cellular response to interferon-gamma. Located in plasma membrane. Part of GAIT complex and aminoacyl-tRNA synthetase multienzyme complex. Is expressed in several structures, including alimentary system; axial skeleton; craniocervical region bone; face; and liver. Human ortholog(s) of this gene implicated in hypomyelinating leukodystrophy. Orthologous to human EPRS1 (glutamyl-prolyl-tRNA synthetase 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in CNS E11.5 (RPKM 17.5), liver E14 (RPKM 12.9) and 25 other tissues See more
Orthologs: human all
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Genomic context

Location:: 1 H5; 1 89.5 cM

Exon count:: 33

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	1	NC_000067.7 (185093614..185160557)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	1	NC_000067.6 (185361417..185428360)

Chromosome 1 - NC_000067.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Cardiomyocyte-Specific Loss of Glutamyl-prolyl-tRNA Synthetase Leads to Disturbed Protein Homeostasis and Dilated Cardiomyopathy.
Title: Cardiomyocyte-Specific Loss of Glutamyl-prolyl-tRNA Synthetase Leads to Disturbed Protein Homeostasis and Dilated Cardiomyopathy.
Glutamyl-Prolyl-tRNA Synthetase Regulates Proline-Rich Pro-Fibrotic Protein Synthesis During Cardiac Fibrosis.
Title: Glutamyl-Prolyl-tRNA Synthetase Regulates Proline-Rich Pro-Fibrotic Protein Synthesis During Cardiac Fibrosis.
The rules presented here for the regulated ribosome bypass of noncanonical initiation codons in the EPRS 5'-leader add complexity into the nature of uORF-mediated translation control mechanisms during eIF2alpha-P and additionally illustrate the roles that previously unexamined uORFs with noncanonical initiation codons can play in modulating gene expression.
Title: Translation Regulation of the Glutamyl-prolyl-tRNA Synthetase Gene EPRS through Bypass of Upstream Open Reading Frames with Noncanonical Initiation Codons.
IFN-gamma activates the macrophage GAIT system via induced phosphorylation of EPRS and L13a. L13a is phosphorylated at Ser(77) by the DAPK-ZIPK cascade, but EPRS is phosphorylated only at Ser(999).
Title: Heterotrimeric GAIT complex drives transcript-selective translation inhibition in murine macrophages.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (5) 1 citation
Endonuclease-mediated (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D9Mit34.1 (e-PCR), detects polymorphism
- UniSTS:131903

D10Chm206 (e-PCR)
- UniSTS:518213

C79379 (e-PCR)
- UniSTS:178541

Eprs (e-PCR), detects polymorphism
- UniSTS:546759

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables GTPase binding	ISO Inferred from Sequence Orthology more info
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables RNA stem-loop binding	ISO Inferred from Sequence Orthology more info
enables aminoacyl-tRNA ligase activity	IEA Inferred from Electronic Annotation more info
enables catalytic activity	IEA Inferred from Electronic Annotation more info
enables glutamate-tRNA ligase activity	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables ligase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables proline-tRNA ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables proline-tRNA ligase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables proline-tRNA ligase activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in cellular response to insulin stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to type II interferon	ISO Inferred from Sequence Orthology more info
involved_in glutamyl-tRNA aminoacylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within metabolic process	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of translation	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of translation	ISO Inferred from Sequence Orthology more info
involved_in prolyl-tRNA aminoacylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in prolyl-tRNA aminoacylation	IDA Inferred from Direct Assay more info	PubMed
involved_in prolyl-tRNA aminoacylation	ISO Inferred from Sequence Orthology more info
involved_in regulation of long-chain fatty acid import into cell	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of long-chain fatty acid import into cell	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of translation	IEA Inferred from Electronic Annotation more info
involved_in tRNA aminoacylation	IEA Inferred from Electronic Annotation more info
involved_in tRNA aminoacylation for protein translation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within translation	IEA Inferred from Electronic Annotation more info
involved_in translation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of aminoacyl-tRNA synthetase multienzyme complex	IBA Inferred from Biological aspect of Ancestor more info
part_of aminoacyl-tRNA synthetase multienzyme complex	IDA Inferred from Direct Assay more info	PubMed
part_of aminoacyl-tRNA synthetase multienzyme complex	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in cytosol	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
part_of ribonucleoprotein complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: bifunctional glutamate/proline--tRNA ligase

Names: bifunctional aminoacyl-tRNA synthetase; glutamine-proline-tRNA synthetase

NP_001344403.1

EC 6.1.1.15

EC 6.1.1.17

NP_084011.1

EC 6.1.1.15

EC 6.1.1.17

XP_017176142.1

EC 6.1.1.15

EC 6.1.1.17

XP_030110720.1

EC 6.1.1.15

EC 6.1.1.17

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001357474.1 → NP_001344403.1 bifunctional glutamate/proline--tRNA ligase isoform 2

Status: VALIDATED

Source sequence(s)

AC129195, AC131980

UniProtKB/TrEMBL

B9EIU1

Conserved Domains (3) summary

PRK08661
Location:1026 → 1519

PRK08661; prolyl-tRNA synthetase; Provisional

cd00936
Location:760 → 809

WEPRS_RNA; WEPRS_RNA binding domain. This short RNA-binding domain is found in several higher eukaryote aminoacyl-tRNA synthetases (aaRSs). It is found in multiple copies in eukaryotic bifunctional glutamyl-prolyl-tRNA synthetases (EPRS) in a region that separates ...

cl27212
Location:6 → 718

GlnS; Glutamyl- or glutaminyl-tRNA synthetase [Translation, ribosomal structure and biogenesis]
NM_029735.2 → NP_084011.1 bifunctional glutamate/proline--tRNA ligase isoform 1

See identical proteins and their annotated locations for NP_084011.1

Status: VALIDATED

Source sequence(s)

AI845980, AK141072, BC058337, BC094679, CB520692, CF537682, CK635189

Consensus CDS

CCDS35818.1

UniProtKB/Swiss-Prot

E9QKC4, Q3UFJ2, Q4VC16, Q8CGC7

UniProtKB/TrEMBL

B9EIU1

Related

ENSMUSP00000045841.8, ENSMUST00000046514.13

Conserved Domains (8) summary

cd10309
Location:74 → 157

GST_C_GluProRS_N; Glutathione S-transferase C-terminal-like, alpha helical domain of bifunctional Glutamyl-Prolyl-tRNA synthetase

PLN02907
Location:6 → 711

PLN02907; glutamate-tRNA ligase

PRK08661
Location:1019 → 1512

PRK08661; prolyl-tRNA synthetase; Provisional

cd00778
Location:1023 → 1286

ProRS_core_arch_euk; Prolyl-tRNA synthetase (ProRS) class II core catalytic domain. ProRS is a homodimer. It is responsible for the attachment of proline to the 3' OH group of ribose of the appropriate tRNA. This domain is primarily responsible for ATP-dependent formation of ...

cd00862
Location:1292 → 1512

ProRS_anticodon_zinc; ProRS Prolyl-anticodon binding domain, long version found predominantly in eukaryotes and archaea. ProRS belongs to class II aminoacyl-tRNA synthetases (aaRS). This alignment contains the anticodon binding domain, which is responsible for specificity in ...

pfam00458
Location:753 → 805

WHEP-TRS; WHEP-TRS domain

pfam00749
Location:197 → 502

tRNA-synt_1c; tRNA synthetases class I (E and Q), catalytic domain

pfam03950
Location:504 → 681

tRNA-synt_1c_C; tRNA synthetases class I (E and Q), anti-codon binding domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000067.7 Reference GRCm39 C57BL/6J

Range

185093614..185160557

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017320653.2 → XP_017176142.1 bifunctional glutamate/proline--tRNA ligase isoform X2

UniProtKB/TrEMBL

B9EIU1

Conserved Domains (4) summary

PLN02907
Location:2 → 454

PLN02907; glutamate-tRNA ligase

PRK08661
Location:762 → 1255

PRK08661; prolyl-tRNA synthetase; Provisional

cd00936
Location:496 → 545

WEPRS_RNA; WEPRS_RNA binding domain. This short RNA-binding domain is found in several higher eukaryote aminoacyl-tRNA synthetases (aaRSs). It is found in multiple copies in eukaryotic bifunctional glutamyl-prolyl-tRNA synthetases (EPRS) in a region that separates ...

pfam00458
Location:569 → 621

WHEP-TRS; WHEP-TRS domain
XM_030254860.2 → XP_030110720.1 bifunctional glutamate/proline--tRNA ligase isoform X1

UniProtKB/TrEMBL

B9EIU1

Conserved Domains (4) summary

PLN02907
Location:2 → 461

PLN02907; glutamate-tRNA ligase

PRK08661
Location:769 → 1262

PRK08661; prolyl-tRNA synthetase; Provisional

cd00936
Location:503 → 552

WEPRS_RNA; WEPRS_RNA binding domain. This short RNA-binding domain is found in several higher eukaryote aminoacyl-tRNA synthetases (aaRSs). It is found in multiple copies in eukaryotic bifunctional glutamyl-prolyl-tRNA synthetases (EPRS) in a region that separates ...

pfam00458
Location:576 → 628

WHEP-TRS; WHEP-TRS domain