Znrf1 zinc and ring finger 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Znrf1provided by MGI
Official Full Name: zinc and ring finger 1provided by MGI
Primary source: MGI:MGI:2177308
See related: Ensembl:ENSMUSG00000033545 AllianceGenome:MGI:2177308
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Rnf42; Zrfp1; nin283; B830022L21Rik
Summary: Enables ubiquitin protein ligase activity. Involved in proteasome-mediated ubiquitin-dependent protein catabolic process and protein K48-linked ubiquitination. Predicted to be located in cytosol; endosome; and membrane. Is expressed in central nervous system; genitourinary system; and retina nuclear layer. Orthologous to human ZNRF1 (zinc and ring finger 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in thymus adult (RPKM 159.2), whole brain E14.5 (RPKM 40.2) and 20 other tissues See more
Orthologs: human all
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Genomic context

Location:: 8 E1; 8 57.98 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	8	NC_000074.7 (112262652..112352352)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	8	NC_000074.6 (111536484..111625720)

Chromosome 8 - NC_000074.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

This study defines ZNRF1 as a regulator of TLR4-induced inflammatory responses and reveals another mechanism for the regulation of TLR4 signalling through CAV1.
Title: The ubiquitin ligase ZNRF1 promotes caveolin-1 ubiquitination and degradation to modulate inflammation.
up-regulation of ZNRF1 activity by oxidative stress leads to neuronal apoptosis and Wallerian degeneration.
Title: Oxidative stress-dependent phosphorylation activates ZNRF1 to induce neuronal/axonal degeneration.
The ZNRF1-AKT-GSK3B-CRMP2 pathway plays an important role in controlling Wallerian degeneration.
Title: ZNRF1 promotes Wallerian degeneration by degrading AKT to induce GSK3B-dependent CRMP2 phosphorylation.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (1) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AA690163 (e-PCR)
- UniSTS:159820

RH143381 (e-PCR)
- UniSTS:232628

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin protein ligase activity	ISO Inferred from Sequence Orthology more info	PubMed
enables ubiquitin-protein transferase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in cytoskeleton organization	TAS Traceable Author Statement more info	PubMed
involved_in positive regulation of toll-like receptor 4 signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in protein K48-linked ubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein K48-linked ubiquitination	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info	PubMed
located_in endosome	ISO Inferred from Sequence Orthology more info
is_active_in intracellular membrane-bounded organelle	IBA Inferred from Biological aspect of Ancestor more info
located_in lysosome	IEA Inferred from Electronic Annotation more info
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	ISO Inferred from Sequence Orthology more info	PubMed
located_in synapse	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: E3 ubiquitin-protein ligase ZNRF1

Names: RING-type E3 ubiquitin transferase ZNRF1; nerve injury-induced gene 283 protein; peripheral nerve injury protein nin283; ring finger protein 42; zinc ring finger protein 1; zinc/RING finger protein 1

NP_001162092.1

EC 2.3.2.27

NP_001162093.1

EC 2.3.2.27

NP_001162094.1

EC 2.3.2.27

NP_001350417.1

EC 2.3.2.27

NP_001350418.1

EC 2.3.2.27

NP_573469.1

EC 2.3.2.27

XP_036009665.1

EC 2.3.2.27

XP_036009666.1

EC 2.3.2.27

XP_036009667.1

EC 2.3.2.27

XP_036009668.1

EC 2.3.2.27

XP_036009669.1

EC 2.3.2.27

XP_036009670.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001168621.3 → NP_001162092.1 E3 ubiquitin-protein ligase ZNRF1 isoform a

See identical proteins and their annotated locations for NP_001162092.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1. Variants 1, 2, and 5 all encode the same isoform (a).

Source sequence(s)

AC122364, AC127356

Consensus CDS

CCDS22676.1

UniProtKB/Swiss-Prot

Q8C5D7, Q91V17

Related

ENSMUSP00000126684.2, ENSMUST00000168428.8

Conserved Domains (1) summary

cd16694
Location:182 → 227

mRING-CH-C4HC2H_ZNRF1; Modified RING-CH finger, H2 subclass (C4HC2H-type), found in zinc/RING finger protein 1 (ZNRF1) and similar proteins
NM_001168622.3 → NP_001162093.1 E3 ubiquitin-protein ligase ZNRF1 isoform b

Status: VALIDATED

Description

Transcript Variant: This variant (3) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.

Source sequence(s)

AC122364, AC127356

Consensus CDS

CCDS52673.1

UniProtKB/TrEMBL

E9PZB7

Related

ENSMUSP00000127956.2, ENSMUST00000171182.8

Conserved Domains (1) summary

cd16694
Location:150 → 195

mRING-CH-C4HC2H_ZNRF1; Modified RING-CH finger, H2 subclass (C4HC2H-type), found in zinc/RING finger protein 1 (ZNRF1) and similar proteins
NM_001168623.2 → NP_001162094.1 E3 ubiquitin-protein ligase ZNRF1 isoform c

Status: VALIDATED

Description

Transcript Variant: This variant (4) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (c) has a shorter and distinct N-terminus compared to isoform a. Variants 4 and 6 both encode the same isoform (c).

Source sequence(s)

AC122364, AC127356

Consensus CDS

CCDS52674.1

UniProtKB/TrEMBL

E9PWL2

Related

ENSMUSP00000132939.2, ENSMUST00000166859.8

Conserved Domains (1) summary

cd16694
Location:82 → 127

mRING-CH-C4HC2H_ZNRF1; Modified RING-CH finger, H2 subclass (C4HC2H-type), found in zinc/RING finger protein 1 (ZNRF1) and similar proteins
NM_001363488.2 → NP_001350417.1 E3 ubiquitin-protein ligase ZNRF1 isoform a

Status: VALIDATED

Description

Transcript Variant: This variant (5) differs in the 3' UTR compared to variant 1. Variants 1, 2, and 5 all encode the same isoform (a).

Source sequence(s)

AC122364, AC127356

Consensus CDS

CCDS22676.1

UniProtKB/Swiss-Prot

Q8C5D7, Q91V17

Related

ENSMUSP00000133993.2, ENSMUST00000173506.8

Conserved Domains (1) summary

cd16694
Location:182 → 227

mRING-CH-C4HC2H_ZNRF1; Modified RING-CH finger, H2 subclass (C4HC2H-type), found in zinc/RING finger protein 1 (ZNRF1) and similar proteins
NM_001363489.1 → NP_001350418.1 E3 ubiquitin-protein ligase ZNRF1 isoform c

Status: VALIDATED

Description

Transcript Variant: This variant (6), as well as variant 4, encodes isoform c.

Source sequence(s)

AC122364, AC127356

Consensus CDS

CCDS52674.1

UniProtKB/TrEMBL

E9PWL2

Related

ENSMUSP00000134634.2, ENSMUST00000174333.8

Conserved Domains (1) summary

cd16694
Location:82 → 127

mRING-CH-C4HC2H_ZNRF1; Modified RING-CH finger, H2 subclass (C4HC2H-type), found in zinc/RING finger protein 1 (ZNRF1) and similar proteins
NM_133206.5 → NP_573469.1 E3 ubiquitin-protein ligase ZNRF1 isoform a

See identical proteins and their annotated locations for NP_573469.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Variants 1, 2, and 5 all encode the same isoform (a).

Source sequence(s)

AC122364, AC127356

Consensus CDS

CCDS22676.1

UniProtKB/Swiss-Prot

Q8C5D7, Q91V17

Related

ENSMUSP00000092799.6, ENSMUST00000095176.12

Conserved Domains (1) summary

cd16694
Location:182 → 227

mRING-CH-C4HC2H_ZNRF1; Modified RING-CH finger, H2 subclass (C4HC2H-type), found in zinc/RING finger protein 1 (ZNRF1) and similar proteins

RNA

NR_156703.2 RNA Sequence

Status: VALIDATED

Description

Transcript Variant: This variant (7) differs in the 3' end compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AC122364, AC127356
NR_156704.2 RNA Sequence

Status: VALIDATED

Description

Transcript Variant: This variant (8) contains an alternate exon and differs in the 3' end compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AC122364, AC127356

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000074.7 Reference GRCm39 C57BL/6J

Range

112262652..112352352

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036153772.1 → XP_036009665.1 E3 ubiquitin-protein ligase ZNRF1 isoform X1

UniProtKB/Swiss-Prot

Q8C5D7, Q91V17

Conserved Domains (1) summary

cd16694
Location:182 → 227

mRING-CH-C4HC2H_ZNRF1; Modified RING-CH finger, H2 subclass (C4HC2H-type), found in zinc/RING finger protein 1 (ZNRF1) and similar proteins
XM_036153773.1 → XP_036009666.1 E3 ubiquitin-protein ligase ZNRF1 isoform X1

UniProtKB/Swiss-Prot

Q8C5D7, Q91V17

Conserved Domains (1) summary

cd16694
Location:182 → 227

mRING-CH-C4HC2H_ZNRF1; Modified RING-CH finger, H2 subclass (C4HC2H-type), found in zinc/RING finger protein 1 (ZNRF1) and similar proteins
XM_036153774.1 → XP_036009667.1 E3 ubiquitin-protein ligase ZNRF1 isoform X1

UniProtKB/Swiss-Prot

Q8C5D7, Q91V17

Related

ENSMUSP00000133309.2, ENSMUST00000172856.8

Conserved Domains (1) summary

cd16694
Location:182 → 227

mRING-CH-C4HC2H_ZNRF1; Modified RING-CH finger, H2 subclass (C4HC2H-type), found in zinc/RING finger protein 1 (ZNRF1) and similar proteins
XM_036153775.1 → XP_036009668.1 E3 ubiquitin-protein ligase ZNRF1 isoform X2

UniProtKB/TrEMBL

E9PZB7

Conserved Domains (1) summary

cd16694
Location:150 → 195

mRING-CH-C4HC2H_ZNRF1; Modified RING-CH finger, H2 subclass (C4HC2H-type), found in zinc/RING finger protein 1 (ZNRF1) and similar proteins
XM_036153776.1 → XP_036009669.1 E3 ubiquitin-protein ligase ZNRF1 isoform X2

UniProtKB/TrEMBL

E9PZB7

Conserved Domains (1) summary

cd16694
Location:150 → 195

mRING-CH-C4HC2H_ZNRF1; Modified RING-CH finger, H2 subclass (C4HC2H-type), found in zinc/RING finger protein 1 (ZNRF1) and similar proteins
XM_036153777.1 → XP_036009670.1 E3 ubiquitin-protein ligase ZNRF1 isoform X2

UniProtKB/TrEMBL

E9PZB7

Conserved Domains (1) summary

cd16694
Location:150 → 195

mRING-CH-C4HC2H_ZNRF1; Modified RING-CH finger, H2 subclass (C4HC2H-type), found in zinc/RING finger protein 1 (ZNRF1) and similar proteins