Pnoc prepronociceptin [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Pnocprovided by MGI
Official Full Name: prepronociceptinprovided by MGI
Primary source: MGI:MGI:105308
See related: Ensembl:ENSMUSG00000045731 AllianceGenome:MGI:105308
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: N/OFQ; Npnc1; OFQ/N
Summary: This gene encodes the precursor for neuropeptides that have been implicated in a wide range of physiological roles such as transmission and sensitivity to pain, learning, memory, anxiety and depression, in the central nervous system. The encoded protein is a precursor that is proteolytically processed to generate multiple biologically active peptides including nociceptin and nocistatin which have opposite functions in pain transmission. Mice lacking the encoded protein display increased anxiety, elevated basal pain threshold and impaired adaptation to repeated stress. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2015]
Expression: Biased expression in whole brain E14.5 (RPKM 9.2), CNS E14 (RPKM 8.2) and 10 other tissues See more
Orthologs: human all
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Genomic context

Location:: 14 D1; 14 34.33 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	14	NC_000080.7 (65638931..65662908, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	14	NC_000080.6 (65400673..65425564, complement)

Chromosome 14 - NC_000080.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Both Pnoc and FoxP1 were expressed at high levels in the rostromedial tegmental nucleus and colocalized strongly with previously established RMTg markers.
Title: Gene expression and neurochemical characterization of the rostromedial tegmental nucleus (RMTg) in rats and mice.
Study demonstrates that nociceptin/orphanin FQ (N/OFQ) activated JNK through the PLC-IP3 signaling pathway, which is coupled to Gi/o, in astrocytes of the spinal dorsal horn. The study also demonstrated that N/OFQ induced monocyte chemoattractant protein-1 release from astrocytes of the spinal cord via JNK activation.
Title: Intrathecal administration of low-dose nociceptin/orphanin FQ induces allodynia via c-Jun N-terminal kinase and monocyte chemoattractant protein-1.
the present results suggest that the endogenous OFQ/N/NOP receptor system may modulate the development of cocaine-induced locomotor sensitization.
Title: The role of NOP receptors in psychomotor stimulation and locomotor sensitization induced by cocaine and amphetamine in mice.
Data suggest that nociceptin/nociceptin receptor signaling plays a role in the disease pathogenesis of inflammatory bowel diseases.
Title: Nociceptin/orphanin FQ inhibition with SB612111 ameliorates dextran sodium sulfate-induced colitis.
these findings may identify nociceptin as a novel paracrine mediator of the FSH effects in the regulation of spermatogenesis.
Title: Nociceptin is upregulated by FSH signaling in Sertoli cells in murine testes.
MPTP increased by about 5-fold the number of neurons expressing ppN/OFQ mRNA in the pars reticulata of the substantia nigra by 24 h after treatment and the elevation remained significant for at least 7 days.
Title: MPTP treatment increases expression of pre-pro-nociceptin/orphanin FQ mRNA in a subset of substantia nigra reticulata neurons.
Endogenous nociceptin/orphanin FQ contributes to reserpine-induced parkinsonism.
Title: Further evidence for an involvement of nociceptin/orphanin FQ in the pathophysiology of Parkinson's disease: a behavioral and neurochemical study in reserpinized mice.
N/OFQ and NOP receptors have an important role in hippocampus-dependent spatial learning and memory, probably by modulation of glutamatergic functions
Title: The nociceptin system and hippocampal cognition in mice: a pharmacological and genetic analysis.
reduction in endogenous N/OFQ, or the lack of its receptor, causes an increase in capsaicin-induced bronchoconstriction, implying a role for the N/OFQ-NOP receptor system in the modulation of capsaicin effects
Title: Nociceptin modulates bronchoconstriction induced by sensory nerve activation in mouse lung.
These results demonstrate the interaction between MrgC and N/OFQ-NOP receptor system in pain transmission.
Title: Involvement of NMDA receptor in nociceptive effects elicited by intrathecal [Tyr6] gamma2-MSH(6-12), and the interaction with nociceptin/orphanin FQ in pain modulation in mice.

Submit: New GeneRIF Correction See all GeneRIFs (20)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (4) 1 citation
Endonuclease-mediated (2)

General gene information

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Markers

NoName (e-PCR)
- UniSTS:491346

Pnoc (e-PCR), detects polymorphism
- UniSTS:144054

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables opioid peptide activity	IEA Inferred from Electronic Annotation more info
enables opioid receptor binding	IBA Inferred from Biological aspect of Ancestor more info
enables receptor ligand activity	IGI Inferred from Genetic Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in chemical synaptic transmission	IBA Inferred from Biological aspect of Ancestor more info
involved_in chemical synaptic transmission	ISO Inferred from Sequence Orthology more info
involved_in neuron-neuron synaptic transmission	IGI Inferred from Genetic Interaction more info	PubMed
involved_in neuropeptide signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in neuropeptide signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in sensory perception	IBA Inferred from Biological aspect of Ancestor more info
involved_in sensory perception of pain	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within signal transduction	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
is_active_in axon terminus	IBA Inferred from Biological aspect of Ancestor more info
is_active_in dendrite	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular region	IEA Inferred from Electronic Annotation more info
is_active_in neuronal cell body	IBA Inferred from Biological aspect of Ancestor more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
is_active_in synaptic membrane	IGI Inferred from Genetic Interaction more info	PubMed

General protein information

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Preferred Names: prepronociceptin

Names: neuropeptide nociceptin 1; orphanin FQ; proorphanin

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001205075.2 → NP_001192004.1 prepronociceptin isoform 2 preproprotein

See identical proteins and their annotated locations for NP_001192004.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) uses an alternate donor splice site at the penultimate exon compared to variant 1. This results in a frameshift, and a longer isoform (2, also known as N27K) with a distinct C-terminus, compared to isoform 1.

Source sequence(s)

AC158983

Consensus CDS

CCDS88690.1

UniProtKB/TrEMBL

B7ZMX9

Related

ENSMUSP00000153589.2, ENSMUST00000224594.2

Conserved Domains (1) summary

pfam01160
Location:21 → 65

Opiods_neuropep; Vertebrate endogenous opioids neuropeptide
NM_001424488.1 → NP_001411417.1 prepronociceptin isoform 1 preproprotein

Status: REVIEWED

Source sequence(s)

AC158983

UniProtKB/Swiss-Prot

Q61105, Q61938, Q64387

UniProtKB/TrEMBL

Q543U6
NM_001424489.1 → NP_001411418.1 prepronociceptin isoform 2 preproprotein

Status: REVIEWED

Source sequence(s)

AC158983

UniProtKB/TrEMBL

B7ZMX9
NM_010932.3 → NP_035062.1 prepronociceptin isoform 1 preproprotein

See identical proteins and their annotated locations for NP_035062.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the shorter protein (isoform 1, also known as N23K).

Source sequence(s)

AC158983

Consensus CDS

CCDS27213.1

UniProtKB/Swiss-Prot

Q61105, Q61938, Q64387

UniProtKB/TrEMBL

Q543U6

Related

ENSMUSP00000054210.6, ENSMUST00000059339.6

Conserved Domains (1) summary

pfam01160
Location:21 → 65

Opiods_neuropep; Vertebrate endogenous opioids neuropeptide