DYNLL1 dynein light chain LC8-type 1 [Homo sapiens (human)] - Gene

Summary

Official Symbol: DYNLL1provided by HGNC
Official Full Name: dynein light chain LC8-type 1provided by HGNC
Primary source: HGNC:HGNC:15476
See related: Ensembl:ENSG00000088986 MIM:601562; AllianceGenome:HGNC:15476
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: LC8; PIN; DLC1; DLC8; LC8a; DNCL1; hdlc1; DNCLC1
Summary: Cytoplasmic dyneins are large enzyme complexes with a molecular mass of about 1,200 kD. They contain two force-producing heads formed primarily from dynein heavy chains, and stalks linking the heads to a basal domain, which contains a varying number of accessory intermediate chains. The complex is involved in intracellular transport and motility. The protein described in this record is a light chain and exists as part of this complex but also physically interacts with and inhibits the activity of neuronal nitric oxide synthase. Binding of this protein destabilizes the neuronal nitric oxide synthase dimer, a conformation necessary for activity, and it may regulate numerous biologic processes through its effects on nitric oxide synthase activity. Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]
Annotation information: Note: DLC1 (Gene ID: 10395) and DYNLL1 (Gene ID: 8655) share the DLC1 symbol/alias in common. DLC1 is sometimes used as an alternative name for dynein light chain LC8-type 1 (DYNLL1), which can be confused with the official symbol for DLC1 Rho GTPase activating protein (DLC1). [05 Jul 2018]
Expression: Ubiquitous expression in brain (RPKM 141.7), esophagus (RPKM 133.4) and 25 other tissues See more
Orthologs: mouse all
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Location:: 12q24.31

Exon count:: 4

Annotation release	Status	Assembly	Chr	Location
RS_2023_10	current	GRCh38.p14 (GCF_000001405.40)	12	NC_000012.12 (120469842..120498493)
RS_2023_10	current	T2T-CHM13v2.0 (GCF_009914755.1)	12	NC_060936.1 (120459024..120487665)
105.20220307	previous assembly	GRCh37.p13 (GCF_000001405.25)	12	NC_000012.11 (120907645..120936296)

Chromosome 12 - NC_000012.12 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help

See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Dynein light chain LC8 alleviates nonalcoholic steatohepatitis by inhibiting NF-kappaB signaling and reducing oxidative stress.
Title: Dynein light chain LC8 alleviates nonalcoholic steatohepatitis by inhibiting NF-κB signaling and reducing oxidative stress.
DYNLL1 is hypomethylated and upregulated in a tumor stage- and grade-dependent manner and associated with increased mortality in hepatocellular carcinoma.
Title: DYNLL1 is hypomethylated and upregulated in a tumor stage- and grade-dependent manner and associated with increased mortality in hepatocellular carcinoma.
The multiple associations of the regulatory proteins of the microtubule network could ensure fine tuning in the regulation of the intracellular trafficking process either by the complexation of DYNLL/LC8 with new partners or indirectly by the modulation of the acetylation level of the microtubule network.
Title: Interactions between two regulatory proteins of microtubule dynamics, HDAC6, TPPP/p25, and the hub protein, DYNLL/LC8.
The data establish LC8 as an important mediator of a subset of 53BP1-dependent DNA double-strand breaks responses.
Title: LC8/DYNLL1 is a 53BP1 effector and regulates checkpoint activation.
identification of recognition motifs for binding partners for the hub protein LC8.
Title: Systematic identification of recognition motifs for the hub protein LC8.
DYNLL1 binds directly to MRE11 to limit its end-resection activity; it is inferred that DYNLL1 is an important anti-resection factor that influences genomic stability and responses to DNA-damaging chemotherapy
Title: DYNLL1 binds to MRE11 to limit DNA end resection in BRCA1-deficient cells.
It has been found that APC2 localizes as distinct clusters along microtubule bundles in dendrites, and that this localization is driven by LC8-binding and two separate microtubule-interacting domains.
Title: APC2 controls dendrite development by promoting microtubule dynamics.
High PIN expression is associated with Dilated Hearts.
Title: Protein Inhibitor of NOS1 Plays a Central Role in the Regulation of NOS1 Activity in Human Dilated Hearts.
An expanded list of LC8 binding partners revealed the evolutionary plasticity of binding partners despite the highly conserved binding interface. In addition, it also highlighted a novel, conserved function of LC8 in the upstream regulation of the Hippo signaling pathway.
Title: Novel linear motif filtering protocol reveals the role of the LC8 dynein light chain in the Hippo pathway.
DLC1 binding to nNOS-calmodulin complex does not affect the electron transport from the reductase to the oxygenase domain.
Title: The protein inhibitor of nNOS (PIN/DLC1/LC8) binding does not inhibit the NADPH-dependent heme reduction in nNOS, a key step in NO synthesis.

Submit: New GeneRIF Correction See all GeneRIFs (48)

Phenotypes

Go to the top of the page Help

Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

Go to the top of the page Help

See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

Go to the top of the page Help

Replication interactions

Interaction	Pubs
Knockdown of dynein light chain 1 (DYNLL1) by shRNA inhibits HIV-1 replication in C8166T cells	PubMed

Protein interactions

Protein	Gene	Interaction	Pubs
Tat	tat	Interaction of HIV-1 Tat with DNCL1 in T-cells is identified by a proteomic strategy based on affinity chromatography	PubMed
capsid	gag	Knockdown of DYNLL1 by shRNA leads to reduced levels of particulate CA in HIV-1-infected cells, suggesting that DYNLL1 plays an important role in CA uncoating	PubMed
integrase	gag-pol	HIV-1 IN mutant Q53A/Q252A is greatly impaired in its ability to interact with PL-DYNLL1 compared to the wild-type	PubMed
	gag-pol	Amino acid residues 50 to 117 and 230 to 288 in HIV-1 IN are essential for interaction with DYNLL1, and two motifs 52GQVD56 and 250VIQD253 in IN closely resemble the consensus sequence for DYNLL1 interaction	PubMed
	gag-pol	The requirement of DYNLL1 for HIV-1 reverse transcription is HIV-1 IN dependent in cells	PubMed
	gag-pol	Co-immunoprecipitation assay demonstrates the direct interaction of HIV-1 IN with DYNLL1 in HIV-1-infected C8166 CD4+ T cells	PubMed
reverse transcriptase	gag-pol	The requirement of DYNLL1 for HIV-1 reverse transcription is HIV-1 IN dependent in cells	PubMed
	gag-pol	Knockdown of DYNLL1 by shRNA results in reduced levels of total HIV-1 DNA and 2-LTR circle DNA compared to control cells, suggesting that DYNLL1 is specifically involved in efficient HIV-1 reverse transcription at early times postinfection	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Markers

NoName (e-PCR)
- UniSTS:489754

Dynll1 (e-PCR), detects polymorphism
- UniSTS:472905

G34959 (e-PCR)
- UniSTS:117669

RH67198 (e-PCR)
- UniSTS:90322

RH80827 (e-PCR)
- UniSTS:84446

G29076 (e-PCR)
- UniSTS:61674

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables dynein intermediate chain binding	IBA Inferred from Biological aspect of Ancestor more info
enables enzyme binding	IEA Inferred from Electronic Annotation more info
enables enzyme inhibitor activity	IDA Inferred from Direct Assay more info	PubMed
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables nitric-oxide synthase inhibitor activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-containing complex binding	IEA Inferred from Electronic Annotation more info
enables scaffold protein binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in intraciliary retrograde transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in motile cilium assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of DNA strand resection involved in replication fork processing	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of nitric oxide biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of insulin secretion involved in cellular response to glucose stimulus	IEA Inferred from Electronic Annotation more info
involved_in spermatid development	IEA Inferred from Electronic Annotation more info
involved_in substantia nigra development	HEP more info	PubMed

Component	Evidence Code	Pubs
part_of COP9 signalosome	IDA Inferred from Direct Assay more info	PubMed
located_in axon cytoplasm	IEA Inferred from Electronic Annotation more info
located_in centrosome	IDA Inferred from Direct Assay more info	PubMed
located_in ciliary tip	TAS Traceable Author Statement more info
located_in cilium	TAS Traceable Author Statement more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
part_of cytoplasmic dynein complex	IBA Inferred from Biological aspect of Ancestor more info
part_of cytoplasmic dynein complex	IDA Inferred from Direct Assay more info	PubMed
part_of cytoplasmic dynein complex	ISS Inferred from Sequence or Structural Similarity more info
located_in cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	TAS Traceable Author Statement more info
part_of dynein complex	IPI Inferred from Physical Interaction more info	PubMed
located_in ficolin-1-rich granule membrane	TAS Traceable Author Statement more info
located_in kinetochore	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	HDA more info	PubMed
located_in microtubule	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
located_in mitotic spindle	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	TAS Traceable Author Statement more info
is_active_in site of double-strand break	IDA Inferred from Direct Assay more info	PubMed
located_in tertiary granule membrane	TAS Traceable Author Statement more info

General protein information

Go to the top of the page Help

Preferred Names: dynein light chain 1, cytoplasmic

Names: 8 kDa dynein light chain; cytoplasmic dynein light polypeptide; dynein, cytoplasmic, light polypeptide 1; protein inhibitor of neuronal nitric oxide synthase

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001037494.2 → NP_001032583.1 dynein light chain 1, cytoplasmic

See identical proteins and their annotated locations for NP_001032583.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longest transcript. . Variants 1, 2 and 3 encode the same protein.

Source sequence(s)

AL021546, BG474448, BI491360, CB992195, CD678447

Consensus CDS

CCDS9200.1

UniProtKB/Swiss-Prot

P63167, Q15701

UniProtKB/TrEMBL

Q6FGH9

Related

ENSP00000376297.2, ENST00000392509.6

Conserved Domains (1) summary

cd21452
Location:5 → 88

DLC-like_DYNLL1_DYNLL2; dynein light chain (DLC)-like domain found in the cytoplasmic dynein light chain 1 (DYNLL1) family
NM_001037495.2 → NP_001032584.1 dynein light chain 1, cytoplasmic

See identical proteins and their annotated locations for NP_001032584.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) contains a distinct 5' UTR including an alternate, downstream transcription initiation site. Variants 1, 2 and 3 encode the same protein.

Source sequence(s)

BC104244, BM817665, CD678447

Consensus CDS

CCDS9200.1

UniProtKB/Swiss-Prot

P63167, Q15701

UniProtKB/TrEMBL

Q6FGH9

Related

ENSP00000376296.2, ENST00000392508.2

Conserved Domains (1) summary

cd21452
Location:5 → 88

DLC-like_DYNLL1_DYNLL2; dynein light chain (DLC)-like domain found in the cytoplasmic dynein light chain 1 (DYNLL1) family
NM_003746.3 → NP_003737.1 dynein light chain 1, cytoplasmic

See identical proteins and their annotated locations for NP_003737.1

Status: REVIEWED

Description

Transcript Variant: This variant (3) contains a distinct 5' UTR, including an alternate, downstream transcription initiation site. Variants 1, 2 and 3 encode the same protein.

Source sequence(s)

AC063943, BU598428

Consensus CDS

CCDS9200.1

UniProtKB/Swiss-Prot

P63167, Q15701

UniProtKB/TrEMBL

Q6FGH9

Related

ENSP00000242577.6, ENST00000242577.11

Conserved Domains (1) summary

cd21452
Location:5 → 88

DLC-like_DYNLL1_DYNLL2; dynein light chain (DLC)-like domain found in the cytoplasmic dynein light chain 1 (DYNLL1) family