Pde5a phosphodiesterase 5A [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Pde5aprovided by RGD
Official Full Name: phosphodiesterase 5Aprovided by RGD
Primary source: RGD:620995
See related: Ensembl:ENSRNOG00000014443 AllianceGenome:RGD:620995
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: PDE5A2
Summary: Enables 3',5'-cyclic-GMP phosphodiesterase activity. Involved in several processes, including blood vessel diameter maintenance; response to testosterone; and short-term memory. Located in cytosol. Used to study artery disease (multiple); impotence (multiple); and primary biliary cholangitis. Human ortholog(s) of this gene implicated in IgA glomerulonephritis. Orthologous to human PDE5A (phosphodiesterase 5A). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Lung (RPKM 466.0), Brain (RPKM 88.6) and 6 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 2q42

Exon count:: 22

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	2	NC_086020.1 (213542822..213687638)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	2	NC_051337.1 (210858515..211003480)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	2	NC_005101.4 (226899604..227044916)

Chromosome 2 - NC_086020.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Changes in the expression and function of the PDE5 pathway in the obstructed urinary bladder.
Title: Changes in the expression and function of the PDE5 pathway in the obstructed urinary bladder.
Differential expression of caveolin-1 during pathogenesis of combined pulmonary fibrosis and emphysema: Effect of phosphodiesterase-5 inhibitor.
Title: Differential expression of caveolin-1 during pathogenesis of combined pulmonary fibrosis and emphysema: Effect of phosphodiesterase-5 inhibitor.
Our data showed a significant and previously undocumented upregulation of PDE5 in both rat and human BPH.
Title: Upregulation of Phosphodiesterase type 5 in the Hyperplastic Prostate.
The effects of vasodilation on plasma volume expansionhemodilution, and PDE5A expression via the renin-angiotensin-aldosterone system are reported.
Title: Chronic vasodilation increases renal medullary PDE5A and α-ENaC through independent renin-angiotensin-aldosterone system pathways.
The phosphodiesterase 5A inhibitors decrease NHE-1 phosphorylation and activity through a mechanism that involves PP2A activation.
Title: Phosphodiesterase 5A inhibition decreases NHE-1 activity without altering steady state pH(i): role of phosphatases.
increased expressions of PKG I and StAR complex, accompanied by decreased PDE5 activity, suggest that NO-cGMP signalling pathway and consequent activation of StAR protein regulate the adaptive response of Leydig cells to repeated immobilization stress.
Title: Structural complexity of the testis and PKG I / StAR interaction regulate the Leydig cell adaptive response to repeated immobilization stress.
PDE5-inhibition blocks TRPC6 channel activation and associated Cn/NFAT activation signaling by PKG-dependent channel phosphorylation
Title: Cyclic GMP/PKG-dependent inhibition of TRPC6 channel activity and expression negatively regulates cardiomyocyte NFAT activation Novel mechanism of cardiac stress modulation by PDE5 inhibition.
phosphorylation and functional suppression of TRPC6 underlie prevention of pathological hypertrophy by PDE5 inhibition.
Title: Phosphorylation of TRPC6 channels at Thr69 is required for anti-hypertrophic effects of phosphodiesterase 5 inhibition.
PDE5 expression was not under direct androgen regulation.
Title: Lack of direct androgen regulation of PDE5 expression.
Phosphodiesterase 5A expression confirms the activity targeted inhibition by sildenafil in cardiomyocytes and hypertrophy modulation.
Title: Expression, activity, and pro-hypertrophic effects of PDE5A in cardiac myocytes.

Submit: New GeneRIF Correction See all GeneRIFs (19)

General gene information

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Markers

Pde5a (e-PCR), detects polymorphism
- UniSTS:546924

Pde5a (e-PCR), detects polymorphism
- UniSTS:507126

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables 3',5'-cyclic-AMP phosphodiesterase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 3',5'-cyclic-GMP phosphodiesterase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 3',5'-cyclic-GMP phosphodiesterase activity	IDA Inferred from Direct Assay more info	PubMed
enables 3',5'-cyclic-GMP phosphodiesterase activity	ISO Inferred from Sequence Orthology more info
enables 3',5'-cyclic-GMP phosphodiesterase activity	TAS Traceable Author Statement more info	PubMed
enables 3',5'-cyclic-nucleotide phosphodiesterase activity	IEA Inferred from Electronic Annotation more info
enables 3',5'-cyclic-nucleotide phosphodiesterase activity	ISO Inferred from Sequence Orthology more info
enables cGMP binding	IEA Inferred from Electronic Annotation more info
enables cGMP binding	ISO Inferred from Sequence Orthology more info
enables cyclic-nucleotide phosphodiesterase activity	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in T cell proliferation	IEA Inferred from Electronic Annotation more info
acts_upstream_of T cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in cAMP-mediated signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in cGMP catabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cGMP catabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cGMP metabolic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of T cell proliferation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within negative regulation of T cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cardiac muscle contraction	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of cardiac muscle contraction	ISO Inferred from Sequence Orthology more info
involved_in nervous system development	IEP Inferred from Expression Pattern more info	PubMed
involved_in oocyte development	IEA Inferred from Electronic Annotation more info
acts_upstream_of oocyte development	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cardiac muscle hypertrophy	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of cardiac muscle hypertrophy	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of chronic inflammatory response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of oocyte development	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of oocyte development	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of vasoconstriction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of nitric oxide mediated signal transduction	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of nitric oxide mediated signal transduction	ISO Inferred from Sequence Orthology more info
involved_in regulation of the force of heart contraction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in relaxation of cardiac muscle	IEA Inferred from Electronic Annotation more info
involved_in relaxation of cardiac muscle	ISO Inferred from Sequence Orthology more info
involved_in response to hypoxia	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to lipopolysaccharide	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to testosterone	IEP Inferred from Expression Pattern more info	PubMed
involved_in short-term memory	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in signal transduction	IEA Inferred from Electronic Annotation more info
involved_in vasodilation	IEP Inferred from Expression Pattern more info	PubMed

General protein information

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Preferred Names: cGMP-specific 3',5'-cyclic phosphodiesterase

Names: CGB-PDE; cGMP-binding cGMP specific phosphodiesterase 5A2; cGMP-binding cGMP-specific phosphodiesterase; phosphodiesterase 5A, cGMP-specific; phosphodiesterase type 5

NP_598268.1

EC 3.1.4.35

XP_006233322.1

EC 3.1.4.35

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_133584.1 → NP_598268.1 cGMP-specific 3',5'-cyclic phosphodiesterase

See identical proteins and their annotated locations for NP_598268.1

Status: PROVISIONAL

Source sequence(s)

D89093

UniProtKB/Swiss-Prot

O54735

UniProtKB/TrEMBL

A6HVH1, G3V7V8

Related

ENSRNOP00000019637.4, ENSRNOT00000019638.5

Conserved Domains (2) summary

smart00065
Location:122 → 279

GAF; Domain present in phytochromes and cGMP-specific phosphodiesterases

pfam00233
Location:570 → 805

PDEase_I; 3'5'-cyclic nucleotide phosphodiesterase

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086020.1 Reference GRCr8

Range

213542822..213687638

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006233260.5 → XP_006233322.1 cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1

See identical proteins and their annotated locations for XP_006233322.1

UniProtKB/TrEMBL

A0A8I6GLL2, A6HVG9, A6HVH0

Related

ENSRNOP00000095374.1

Conserved Domains (2) summary

smart00065
Location:154 → 311

GAF; Domain present in phytochromes and cGMP-specific phosphodiesterases

pfam00233
Location:602 → 837

PDEase_I; 3'5'-cyclic nucleotide phosphodiesterase