ICE1 basic helix-loop-helix (bHLH) DNA-binding superfamily protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Gene symbol: ICE1
Gene description: basic helix-loop-helix (bHLH) DNA-binding superfamily protein
Primary source: Araport:AT3G26744
Locus tag: AT3G26744
See related: TAIR:AT3G26744
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: A. THALIANA INDUCER OF CBP EXPRESSION 1; ATICE1; INDUCER OF CBF EXPRESSION 1; SCREAM; SCRM
Summary: Encodes a MYC-like bHLH transcriptional activator that binds specifically to the MYC recognition sequences in the CBF3 promoter. Mutants are defective in cold-regulated gene expression. Cold stress triggers protein degradation of nuclear GFPICE1 protein, and the RING finger protein HOS1 is required. Sumoylation of ICE1 controls CBF3/DREB1A expression and freezing tolerance.
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Genomic context

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See ICE1 in Genome Data Viewer

Location:: chromosome: 3

Exon count:: 4

Sequence:: Chromosome: 3; NC_003074.8 (9832667..9835279, complement)

Chromosome 3 - NC_003074.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003074.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The transcription factor MYB43 antagonizes with ICE1 to regulate freezing tolerance in Arabidopsis.
Title: The transcription factor MYB43 antagonizes with ICE1 to regulate freezing tolerance in Arabidopsis.
ATBS1-INTERACTING FACTOR 2 negatively regulates dark- and brassinosteroid-induced leaf senescence through interactions with INDUCER OF CBF EXPRESSION 1.
Title: ATBS1-INTERACTING FACTOR 2 negatively regulates dark- and brassinosteroid-induced leaf senescence through interactions with INDUCER OF CBF EXPRESSION 1.
DREB1A/CBF3 Is Repressed by Transgene-Induced DNA Methylation in the Arabidopsis ice1 -1 Mutant.
Title: DREB1A/CBF3 Is Repressed by Transgene-Induced DNA Methylation in the Arabidopsis ice1 -1 Mutant.
Protein kinase BRASSINOSTEROID-INSENSITIVE2 (BIN2) interacts with and phosphorylates INDUCER OF CBF EXPRESSION1 (ICE1) in Arabidopsis (Arabidopsis thaliana) under prolonged cold stress, facilitating the interaction between ICE1 and the E3 ubiquitin ligase HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE1 and thereby promoting ICE1 degradation.
Title: BRASSINOSTEROID-INSENSITIVE2 Negatively Regulates the Stability of Transcription Factor ICE1 in Response to Cold Stress in Arabidopsis.
ICE1 antagonizes ABI5 and DELLA activity to maintain the appropriate level of ABA signaling during seed germination.
Title: The Transcription Factor INDUCER OF CBF EXPRESSION1 Interacts with ABSCISIC ACID INSENSITIVE5 and DELLA Proteins to Fine-Tune Abscisic Acid Signaling during Seed Germination in Arabidopsis.
SCREAM functions as a scaffold that recruits MPK3/6 to downregulate SPEECHLESS, a transcription factor that initiates stomatal cell lineages.
Title: Bipartite anchoring of SCREAM enforces stomatal initiation by coupling MAP kinases to SPEECHLESS.
Arabidopsis ICE1 and ZOU determine the depth of primary dormancy during maturation independently of their effect on endosperm development.
Title: ICE1 and ZOU determine the depth of primary seed dormancy in Arabidopsis independently of their role in endosperm development.
these results demonstrate that MYC67 and MYC70, ICE1 interactors, negatively regulate cold-responsive genes and cold tolerance.
Title: MYC-type transcription factors, MYC67 and MYC70, interact with ICE1 and negatively regulate cold tolerance in Arabidopsis.
AtICE1 methylation-regulated transcription of CBF pathway genes is responsible for the phenotypic variation in the freezing tolerance observed in A. thaliana.
Title: Variation in ICE1 Methylation Primarily Determines Phenotypic Variation in Freezing Tolerance in Arabidopsis thaliana.
Data indicate that light is directly linked with the inducer of CBF Expression (ICE) transcription factors (ICE)-directed signaling module, via the CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1)-mediated protein surveillance system, in the modulation of stomatal development.
Title: Light Inhibits COP1-Mediated Degradation of ICE Transcription Factors to Induce Stomatal Development in Arabidopsis.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_001035697.2

Supported by ESTs:

NM_001035699.2

Supported by ESTs:

NM_001338833.1

Supported by ESTs:

NM_001338834.1

Supported by ESTs:

NM_113586.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	TAS Traceable Author Statement more info	PubMed
enables DNA-binding transcription factor activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables cis-regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables core promoter sequence-specific DNA binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein dimerization activity	IEA Inferred from Electronic Annotation more info
enables transcription cis-regulatory region binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within abscisic acid-activated signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within endosperm development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within negative regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of abscisic acid biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to cold	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to freezing	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within seed dormancy process	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within seed dormancy process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within seed morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within seed morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within stomatal lineage progression	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISM Inferred from Sequence Model more info

General protein information

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Preferred Names: basic helix-loop-helix (bHLH) DNA-binding superfamily protein

NP_001030774.1

INDUCER OF CBF EXPRESSION 1 (ICE1); CONTAINS InterPro DOMAIN/s: Helix-loop-helix DNA-binding domain (InterPro:IPR001092), Helix-loop-helix DNA-binding (InterPro:IPR011598); BEST Arabidopsis thaliana protein match is: basic helix-loop-helix (bHLH) DNA-binding superfamily protein (TAIR:AT1G12860.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).

NP_001030776.2

INDUCER OF CBF EXPRESSION 1 (ICE1); CONTAINS InterPro DOMAIN/s: Helix-loop-helix DNA-binding domain (InterPro:IPR001092), Helix-loop-helix DNA-binding (InterPro:IPR011598); BEST Arabidopsis thaliana protein match is: basic helix-loop-helix (bHLH) DNA-binding superfamily protein (TAIR:AT1G12860.1); Has 2623 Blast hits to 2616 proteins in 144 species: Archae - 0; Bacteria - 0; Metazoa - 19; Fungi - 30; Plants - 2572; Viruses - 0; Other Eukaryotes - 2 (source: NCBI BLink).

NP_189309.2

INDUCER OF CBF EXPRESSION 1 (ICE1); CONTAINS InterPro DOMAIN/s: Helix-loop-helix DNA-binding domain (InterPro:IPR001092), Helix-loop-helix DNA-binding (InterPro:IPR011598); BEST Arabidopsis thaliana protein match is: basic helix-loop-helix (bHLH) DNA-binding superfamily protein (TAIR:AT1G12860.1); Has 2623 Blast hits to 2616 proteins in 144 species: Archae - 0; Bacteria - 0; Metazoa - 19; Fungi - 30; Plants - 2572; Viruses - 0; Other Eukaryotes - 2 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003074.8 Reference assembly

Range

9832667..9835279 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001035697.2 → NP_001030774.1 basic helix-loop-helix (bHLH) DNA-binding superfamily protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001030774.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q0WR77, Q2V3S3, Q5PNR8, Q9LSE2

UniProtKB/TrEMBL

A0A0D5MF12, A0A178VDZ0, A0A384KCX7

Conserved Domains (2) summary

cd04873
Location:423 → 483

ACT_UUR-ACR-like; ACT domains of the bacterial signal-transducing uridylyltransferase /uridylyl-removing (UUR) enzyme, GlnD

cd11443
Location:306 → 377

bHLH_AtAMS_like; basic helix-loop-helix (bHLH) domain found in Arabidopsis thaliana protein aborted microspores (AMS) and similar proteins
NM_001338834.1 → NP_001327171.1 basic helix-loop-helix (bHLH) DNA-binding superfamily protein [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A1I9LRH4
NM_001338833.1 → NP_001327170.1 basic helix-loop-helix (bHLH) DNA-binding superfamily protein [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9LSE2
NM_113586.4 → NP_189309.2 basic helix-loop-helix (bHLH) DNA-binding superfamily protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_189309.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q0WR77, Q2V3S3, Q5PNR8, Q9LSE2

UniProtKB/TrEMBL

A0A0D5MF12, A0A178VDZ0, A0A384KCX7

Conserved Domains (2) summary

cd04873
Location:423 → 483

ACT_UUR-ACR-like; ACT domains of the bacterial signal-transducing uridylyltransferase /uridylyl-removing (UUR) enzyme, GlnD

cd11443
Location:306 → 377

bHLH_AtAMS_like; basic helix-loop-helix (bHLH) domain found in Arabidopsis thaliana protein aborted microspores (AMS) and similar proteins
NM_001035699.2 → NP_001030776.2 basic helix-loop-helix (bHLH) DNA-binding superfamily protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001030776.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q0WR77, Q2V3S3, Q5PNR8, Q9LSE2

UniProtKB/TrEMBL

A0A0D5MF12, A0A178VDZ0, A0A384KCX7

Conserved Domains (2) summary

cd04873
Location:423 → 483

ACT_UUR-ACR-like; ACT domains of the bacterial signal-transducing uridylyltransferase /uridylyl-removing (UUR) enzyme, GlnD

cd11443
Location:306 → 377

bHLH_AtAMS_like; basic helix-loop-helix (bHLH) domain found in Arabidopsis thaliana protein aborted microspores (AMS) and similar proteins