Mcm4 minichromosome maintenance complex component 4 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Mcm4provided by MGI
Official Full Name: minichromosome maintenance complex component 4provided by MGI
Primary source: MGI:MGI:103199
See related: Ensembl:ENSMUSG00000022673 AllianceGenome:MGI:103199
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 19G; Cdc21; Mcmd4; mcdc21; mKIAA4003
Summary: Enables single-stranded DNA binding activity. Contributes to DNA helicase activity. Acts upstream of or within DNA unwinding involved in DNA replication. Predicted to be located in nucleoplasm. Predicted to be part of MCM complex. Predicted to be active in nucleus. Is expressed in several structures, including brain; future brain; limb; metanephros; and myocardium layer. Human ortholog(s) of this gene implicated in immunodeficiency 54. Orthologous to human MCM4 (minichromosome maintenance complex component 4). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in liver E14 (RPKM 36.5), liver E14.5 (RPKM 34.4) and 21 other tissues See more
Orthologs: human all
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Genomic context

Location:: 16 A2; 16 10.09 cM

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	16	NC_000082.7 (15441761..15455264, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	16	NC_000082.6 (15623897..15637400, complement)

Chromosome 16 - NC_000082.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

E2F2 is upregulated by the ERK pathway and regulates decidualization via MCM4.
Title: E2F2 is upregulated by the ERK pathway and regulates decidualization via MCM4.
Together, these data support a model in which chromosomal abnormalities in Sdl mice result from the ability of MCM4(D573H) to incorporate into MCM complexes and render them inactive.
Title: A dominantly acting murine allele of Mcm4 causes chromosomal abnormalities and promotes tumorigenesis.
Peroxisome proliferator-activated receptor gamma coactivator 1beta (PGC-1beta) protein attenuates vascular lesion formation by inhibition of chromatin loading of minichromosome maintenance complex in smooth muscle cells
Title: Peroxisome proliferator-activated receptor γ coactivator 1β (PGC-1β) protein attenuates vascular lesion formation by inhibition of chromatin loading of minichromosome maintenance complex in smooth muscle cells.
MCM4 mutation may have a role in adrenal failure, short stature, and natural killer cell deficiency
Title: MCM4 mutation causes adrenal failure, short stature, and natural killer cell deficiency in humans.
partial MCM4 deficiency results in a genetic syndrome of growth retardation with adrenal insufficiency and selective NK deficiency
Title: Partial MCM4 deficiency in patients with growth retardation, adrenal insufficiency, and natural killer cell deficiency.
DNA binding and helicase activities of Mcm4/6/7 are significantly stimulated by Cdt1
Title: Cdt1 forms a complex with the minichromosome maintenance protein (MCM) and activates its helicase activity.
Loss of one allele of cdc2l genefacilitates carcinogen-induced skin carcinogenesis in vivo.
Title: Haploinsufficiency of the cdc2l gene contributes to skin cancer development in mice.
Mcm4(Chaos3) mutation appears to destabilize the MCM2-7 complex, causing impaired DNA replication and may have a causative role in cancer development.
Title: Genetic screen for chromosome instability in mice: Mcm4 and breast cancer.
Substrate preference and helicase actions of mouse MCM4/6/7 helicase complexes.
Title: DNA binding and helicase actions of mouse MCM4/6/7 helicase.
MCM4 phosphorylation by Cdc7 kinase facilitates its interaction with Cdc45 on chromatin
Title: Phosphorylation of MCM4 by Cdc7 kinase facilitates its interaction with Cdc45 on the chromatin.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Spontaneous (1) 1 citation
Gene trapped (1) 1 citation
Targeted (2)
Chemically induced (ENU) (1) 1 citation
Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AU045576 (e-PCR)
- UniSTS:160531

AI325074 (e-PCR)
- UniSTS:180870

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
contributes_to DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
contributes_to DNA helicase activity	IMP Inferred from Mutant Phenotype more info	PubMed
contributes_to DNA helicase activity	ISO Inferred from Sequence Orthology more info
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables single-stranded DNA binding	IBA Inferred from Biological aspect of Ancestor more info
contributes_to single-stranded DNA binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables single-stranded DNA binding	IPI Inferred from Physical Interaction more info	PubMed
contributes_to single-stranded DNA binding	ISO Inferred from Sequence Orthology more info
contributes_to single-stranded DNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in DNA duplex unwinding	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA replication initiation	IEA Inferred from Electronic Annotation more info
involved_in DNA strand elongation involved in DNA replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA unwinding involved in DNA replication	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within DNA unwinding involved in DNA replication	IPI Inferred from Physical Interaction more info	PubMed
involved_in DNA unwinding involved in DNA replication	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cell cycle	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via break-induced replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic DNA replication initiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in premeiotic DNA replication	NAS Non-traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
part_of CMG complex	ISO Inferred from Sequence Orthology more info
part_of MCM complex	IBA Inferred from Biological aspect of Ancestor more info
part_of MCM complex	IDA Inferred from Direct Assay more info	PubMed
part_of MCM complex	ISO Inferred from Sequence Orthology more info
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	NAS Non-traceable Author Statement more info	PubMed

General protein information

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Preferred Names: DNA replication licensing factor MCM4

Names: CDC21 homolog; P1-CDC21; mini chromosome maintenance deficient 4 homolog; minichromosome maintenance deficient 4 homolog

NP_032591.3

EC 3.6.4.12

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_008565.3 → NP_032591.3 DNA replication licensing factor MCM4

See identical proteins and their annotated locations for NP_032591.3

Status: VALIDATED

Source sequence(s)

AK036653, AK141644, AK151499

Consensus CDS

CCDS27977.1

UniProtKB/Swiss-Prot

O89056, P49717

UniProtKB/TrEMBL

Q3UA65, Q542F4, Q921D5

Related

ENSMUSP00000023353.4, ENSMUST00000023353.4

Conserved Domains (3) summary

smart00350
Location:266 → 769

MCM; minichromosome maintenance proteins

pfam14551
Location:165 → 286

MCM_N; MCM N-terminal domain

cl21455
Location:504 → 643

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases