Gls2 glutaminase 2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Gls2provided by RGD
Official Full Name: glutaminase 2provided by RGD
Primary source: RGD:620359
See related: Ensembl:ENSRNOG00000031612 AllianceGenome:RGD:620359
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Ga
Summary: Predicted to enable glutaminase activity. Predicted to be involved in glutamate biosynthetic process and glutamine catabolic process. Predicted to act upstream of or within reactive oxygen species metabolic process and regulation of apoptotic process. Predicted to be located in mitochondrion. Orthologous to human GLS2 (glutaminase 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Liver (RPKM 464.5), Brain (RPKM 70.6) and 2 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 7q11

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	7	NC_086025.1 (1201757..1218014)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	7	NC_051342.1 (617252..633424)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	7	NC_005106.4 (2605679..2621853)

Chromosome 7 - NC_086025.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

miR-190a-5p regulates cardiomyocytes response to ferroptosis via directly targeting GLS2.
Title: miR-190a-5p regulates cardiomyocytes response to ferroptosis via directly targeting GLS2.
GABAergic neurons and astrocytes express Gls and Gls2 isoenzymes in nucleus and mitochondria, in addition to glutamatergic neurons
Title: Expression of Gls and Gls2 glutaminase isoforms in astrocytes.
In animals with chronic hepatitis; hyperbaric oxygenation restricted the stimulating effect of hepatic resection on phosphate-dependent glutaminase activity.
Title: Phosphate-dependent glutaminase response to liver injury and hyperbaric oxygenation.
Study demonstrated expression of alternative transcripts of the mammalian Gls2 gene. Transcriptional mechanisms giving rise to GLS2 variants and isolation of novel GLS2 transcripts in human, rat and mouse are presented.
Title: Mammalian glutaminase Gls2 gene encodes two functional alternative transcripts by a surrogate promoter usage mechanism.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables glutaminase activity	IBA Inferred from Biological aspect of Ancestor more info
enables glutaminase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in glutamate biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in glutamine catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in reactive oxygen species metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within reactive oxygen species metabolic process	ISO Inferred from Sequence Orthology more info
involved_in regulation of apoptotic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of apoptotic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in mitochondrial matrix	TAS Traceable Author Statement more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: glutaminase liver isoform, mitochondrial

Names: GLS; L-glutaminase; L-glutamine amidohydrolase; glutaminase 2 (liver, mitochondrial); liver mitochondrial glutaminase

NP_001257715.1

EC 3.5.1.2

NP_001257716.1

EC 3.5.1.2

NP_620259.2

EC 3.5.1.2

XP_006240790.1

EC 3.5.1.2

XP_006240792.1

EC 3.5.1.2

XP_017450141.1

EC 3.5.1.2

XP_017450142.1

EC 3.5.1.2

XP_038934314.1

EC 3.5.1.2

XP_038934315.1

EC 3.5.1.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001270786.1 → NP_001257715.1 glutaminase liver isoform, mitochondrial isoform 1

See identical proteins and their annotated locations for NP_001257715.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longest isoform (1).

Source sequence(s)

BC089776, JAXUCZ010000007

UniProtKB/Swiss-Prot

P28492, Q3MHS6, Q5FVU0, Q64606

UniProtKB/TrEMBL

A0A0S2EF23, A0A8I6GM40

Related

ENSRNOP00000097543.1, ENSRNOT00000110409.1

Conserved Domains (4) summary

cd00204
Location:490 → 578

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam04960
Location:177 → 463

Glutaminase; Glutaminase

pfam13637
Location:520 → 572

Ank_4; Ankyrin repeats (many copies)

sd00045
Location:490 → 516

ANK; ANK repeat [structural motif]
NM_001270787.1 → NP_001257716.1 glutaminase liver isoform, mitochondrial isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate splice site at the 3' end of the first exon compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1.

Source sequence(s)

BC089776, JAXUCZ010000007

UniProtKB/TrEMBL

A0A0H2UHL2, A0A8I6AH34

Related

ENSRNOP00000018737.5, ENSRNOT00000018737.6

Conserved Domains (4) summary

cd00204
Location:467 → 555

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam04960
Location:154 → 440

Glutaminase; Glutaminase

pfam13637
Location:497 → 549

Ank_4; Ankyrin repeats (many copies)

sd00045
Location:467 → 493

ANK; ANK repeat [structural motif]
NM_138904.2 → NP_620259.2 glutaminase liver isoform, mitochondrial isoform 3

See identical proteins and their annotated locations for NP_620259.2

Status: VALIDATED

Description

Transcript Variant: This variant (3) lacks the first exon and extends 5' into intronic sequence compared to variant 1. The resulting isoform (3) is shorter at the N-terminus compared to isoform 1.

Source sequence(s)

BC089776, JAXUCZ010000007

UniProtKB/TrEMBL

A0A8I6AH34, A6KSA7

Conserved Domains (4) summary

cd00204
Location:423 → 511

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam04960
Location:110 → 396

Glutaminase; Glutaminase

pfam13637
Location:453 → 505

Ank_4; Ankyrin repeats (many copies)

sd00045
Location:423 → 449

ANK; ANK repeat [structural motif]

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086025.1 Reference GRCr8

Range

1201757..1218014

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006240728.5 → XP_006240790.1 glutaminase liver isoform, mitochondrial isoform X1

UniProtKB/TrEMBL

A0A0S2EF05, A0A8I6GM40

Related

ENSRNOP00000087957.1

Conserved Domains (4) summary

cd00204
Location:459 → 547

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam04960
Location:177 → 452

Glutaminase; Glutaminase

pfam13637
Location:489 → 541

Ank_4; Ankyrin repeats (many copies)

sd00045
Location:459 → 485

ANK; ANK repeat [structural motif]
XM_017594652.3 → XP_017450141.1 glutaminase liver isoform, mitochondrial isoform X3

UniProtKB/TrEMBL

A0A0S2EF12, A0A8I6ACB5

Related

ENSRNOP00000076539.1

Conserved Domains (4) summary

cd00204
Location:416 → 504

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam04960
Location:103 → 389

Glutaminase; Glutaminase

pfam13637
Location:446 → 498

Ank_4; Ankyrin repeats (many copies)

sd00045
Location:416 → 442

ANK; ANK repeat [structural motif]
XM_017594653.2 → XP_017450142.1 glutaminase liver isoform, mitochondrial isoform X4

UniProtKB/TrEMBL

A0A0S2EFM6

Related

ENSRNOP00000091564.1, ENSRNOT00000099901.1
XM_039078386.1 → XP_038934314.1 glutaminase liver isoform, mitochondrial isoform X5

UniProtKB/TrEMBL

A6KSA8

Conserved Domains (3) summary

sd00045
Location:301 → 327

ANK; ANK repeat [structural motif]

pfam04960
Location:17 → 272

Glutaminase

pfam12796
Location:301 → 383

Ank_2; Ankyrin repeats (3 copies)
XM_039078387.2 → XP_038934315.1 glutaminase liver isoform, mitochondrial isoform X5

UniProtKB/TrEMBL

A6KSA8

Conserved Domains (3) summary

sd00045
Location:301 → 327

ANK; ANK repeat [structural motif]

pfam04960
Location:17 → 272

Glutaminase

pfam12796
Location:301 → 383

Ank_2; Ankyrin repeats (3 copies)
XM_006240730.4 → XP_006240792.1 glutaminase liver isoform, mitochondrial isoform X2

See identical proteins and their annotated locations for XP_006240792.1

UniProtKB/TrEMBL

A0A8I6AH34, A6KSA7

Conserved Domains (4) summary

cd00204
Location:423 → 511

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam04960
Location:110 → 396

Glutaminase; Glutaminase

pfam13637
Location:453 → 505

Ank_4; Ankyrin repeats (many copies)

sd00045
Location:423 → 449

ANK; ANK repeat [structural motif]