Spns2 SPNS lysolipid transporter 2, sphingosine-1-phosphate [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Spns2provided by MGI
Official Full Name: SPNS lysolipid transporter 2, sphingosine-1-phosphateprovided by MGI
Primary source: MGI:MGI:2384936
See related: Ensembl:ENSMUSG00000040447 AllianceGenome:MGI:2384936
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Summary: Predicted to enable sphingolipid transporter activity. Acts upstream of or within several processes, including lymph node development; lymphocyte homeostasis; and sphingosine-1-phosphate receptor signaling pathway. Predicted to be located in endosome and plasma membrane. Predicted to be integral component of membrane. Is expressed in jaw bone; nervous system; and sensory organ. Human ortholog(s) of this gene implicated in autosomal recessive nonsyndromic deafness 115. Orthologous to human SPNS2 (sphingolipid transporter 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in lung adult (RPKM 39.6), kidney adult (RPKM 31.5) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 B4; 11 44.31 cM

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (72342464..72380797, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (72451638..72489976, complement)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Anomaly of cornea and ocular adnexa in spinster homolog 2 (Spns2) knockout mice.
Title: Anomaly of cornea and ocular adnexa in spinster homolog 2 (Spns2) knockout mice.
Postnatal deletion of Spns2 prevents neuroinflammation without compromising blood vascular functions.
Title: Postnatal deletion of Spns2 prevents neuroinflammation without compromising blood vascular functions.
Mfsd2b and Spns2 are essential for maintenance of blood vessels during development and in anaphylactic shock.
Title: Mfsd2b and Spns2 are essential for maintenance of blood vessels during development and in anaphylactic shock.
SPNS2 enables T cell egress from lymph nodes during an immune response.
Title: SPNS2 enables T cell egress from lymph nodes during an immune response.
Endothelial Spns2 and ApoM Regulation of Vascular Tone and Hypertension Via Sphingosine-1-Phosphate.
Title: Endothelial Spns2 and ApoM Regulation of Vascular Tone and Hypertension Via Sphingosine-1-Phosphate.
In summary, these results suggest that Spns2 promotes pro-inflammatory polarization of microglia and may play a crucial role in Alzheimer disease pathogenesis.
Title: Lipid transporter Spns2 promotes microglia pro-inflammatory activation in response to amyloid-beta peptide.
SPNS2 is essential for proper postnatal retinal morphogenesis.
Title: S1P transporter SPNS2 regulates proper postnatal retinal morphogenesis.
genome-wide in vivo screen identifies novel host regulators of metastatic colonization, such as Spns2
Title: Genome-wide in vivo screen identifies novel host regulators of metastatic colonization.
results point to a broad role for Spns2-mediated S1P transport in the initiation and development of adaptive immune related disorders
Title: Spinster 2, a sphingosine-1-phosphate transporter, plays a critical role in inflammatory and autoimmune diseases.
Spns2 expression by endothelial cells is essential for secretion of lymph S1P, while Spns2 is dispensable for RBC secretion of plasma S1P.
Title: The transporter Spns2 is required for secretion of lymph but not plasma sphingosine-1-phosphate.

Submit: New GeneRIF Correction See all GeneRIFs (15)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (7) 1 citation
Endonuclease-mediated (4)

General gene information

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Markers

RH125539 (e-PCR)
- UniSTS:162354

RH136313 (e-PCR)
- UniSTS:210305

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables sphingolipid transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables sphingolipid transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables sphingolipid transporter activity	ISO Inferred from Sequence Orthology more info
enables transmembrane transporter activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within B cell homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within T cell homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within bone development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lipid transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in lipid transport	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within lymph node development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within lymphocyte homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within lymphocyte migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of T cell migration	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of eye pigmentation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of humoral immune response	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of humoral immune response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sensory perception of sound	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within sphingolipid metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sphingosine-1-phosphate receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in sphingosine-1-phosphate receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within sphingosine-1-phosphate receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transmembrane transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in endosome	IEA Inferred from Electronic Annotation more info
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: sphingosine-1-phosphate transporter SPNS2

Names: protein spinster homolog 2; spinster homolog 2; spinster-like protein; spinster2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001276383.1 → NP_001263312.1 sphingosine-1-phosphate transporter SPNS2 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) contains an alternate exon in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1.

Source sequence(s)

AL662812

UniProtKB/TrEMBL

Q3U1F5

Conserved Domains (1) summary

cd17328
Location:1 → 369

MFS_spinster_like; Protein spinster and spinster homologs of the Major Facilitator Superfamily of transporters
NM_153060.3 → NP_694700.2 sphingosine-1-phosphate transporter SPNS2 isoform 1

See identical proteins and their annotated locations for NP_694700.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AL662812

Consensus CDS

CCDS48841.1

UniProtKB/Swiss-Prot

B9A1T4, Q5F242, Q8R119, Q91VM4

UniProtKB/TrEMBL

Q3U1F5

Related

ENSMUSP00000044418.4, ENSMUST00000045303.10

Conserved Domains (1) summary

cd17328
Location:106 → 520

MFS_spinster_like; Protein spinster and spinster homologs of the Major Facilitator Superfamily of transporters

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000077.7 Reference GRCm39 C57BL/6J

Range

72342464..72380797 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006532935.3 → XP_006532998.1 sphingosine-1-phosphate transporter SPNS2 isoform X2

Conserved Domains (1) summary

cd17328
Location:106 → 448

MFS_spinster_like; Protein spinster and spinster homologs of the Major Facilitator Superfamily of transporters
XM_017314460.3 → XP_017169949.1 sphingosine-1-phosphate transporter SPNS2 isoform X4

UniProtKB/Swiss-Prot

B9A1T4, Q5F242, Q8R119, Q91VM4

UniProtKB/TrEMBL

Q3U1F5

Conserved Domains (1) summary

cd17328
Location:106 → 520

MFS_spinster_like; Protein spinster and spinster homologs of the Major Facilitator Superfamily of transporters
XM_006532934.3 → XP_006532997.1 sphingosine-1-phosphate transporter SPNS2 isoform X1

See identical proteins and their annotated locations for XP_006532997.1

Conserved Domains (1) summary

cd17328
Location:106 → 520

MFS_spinster_like; Protein spinster and spinster homologs of the Major Facilitator Superfamily of transporters
XM_006532936.5 → XP_006532999.1 sphingosine-1-phosphate transporter SPNS2 isoform X3

Conserved Domains (1) summary

cd17328
Location:1 → 369

MFS_spinster_like; Protein spinster and spinster homologs of the Major Facilitator Superfamily of transporters