Mfge8 milk fat globule EGF and factor V/VIII domain containing [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Mfge8provided by RGD
Official Full Name: milk fat globule EGF and factor V/VIII domain containingprovided by RGD
Primary source: RGD:3083
See related: Ensembl:ENSRNOG00000017510 AllianceGenome:RGD:3083
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: AGS; MFGME8; OAcGD3S; MFGMP-E8
Summary: Predicted to enable integrin binding activity; phosphatidylethanolamine binding activity; and phosphatidylserine binding activity. Involved in positive regulation of cell population proliferation and response to estrogen. Predicted to be located in external side of plasma membrane and extracellular space. Predicted to be extrinsic component of plasma membrane. Human ortholog(s) of this gene implicated in temporal arteritis. Orthologous to human MFGE8 (milk fat globule EGF and factor V/VIII domain containing). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Lung (RPKM 1004.7), Spleen (RPKM 992.9) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 1q31

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	1	NC_086019.1 (142474027..142489431, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	1	NC_051336.1 (133064665..133080069, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	1	NC_005100.4 (140845478..140860882, complement)

Chromosome 1 - NC_086019.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

TMT-based quantitative proteomics analysis reveals the effect of bovine derived MFG-E8 against oxidative stress on rat L6 cells.
Title: TMT-based quantitative proteomics analysis reveals the effect of bovine derived MFG-E8 against oxidative stress on rat L6 cells.
MFGE8 is down-regulated in cardiac fibrosis and attenuates endothelial-mesenchymal transition through Smad2/3-Snail signalling pathway.
Title: MFGE8 is down-regulated in cardiac fibrosis and attenuates endothelial-mesenchymal transition through Smad2/3-Snail signalling pathway.
MFGE8 attenuates Ang-II-induced atrial fibrosis and vulnerability to atrial fibrillation through inhibition of TGF-beta1/Smad2/3 pathway.
Title: MFGE8 attenuates Ang-II-induced atrial fibrosis and vulnerability to atrial fibrillation through inhibition of TGF-β1/Smad2/3 pathway.
Needle-shaped amyloid deposition in rat mammary gland: evidence of a novel amyloid fibril protein.
Title: Needle-shaped amyloid deposition in rat mammary gland: evidence of a novel amyloid fibril protein.
Abolished the effect of rhMFG-E8 on anti-apoptosis.
Title: Recombinant milk fat globule-EGF factor-8 reduces apoptosis via integrin β3/FAK/PI3K/AKT signaling pathway in rats after traumatic brain injury.
beta-amyloid peptide induced neuronal loss is mediated by the bridging protein lactadherin/milk-fat globule epidermal growth factor-like factor 8
Title: Lactadherin/MFG-E8 is essential for microglia-mediated neuronal loss and phagoptosis induced by amyloid β.
Liver and plasma MFG-E8 protein levels were significantly decreased after hepatic ischemia-reperfusion
Title: Milk fat globule--EGF factor VIII ameliorates liver injury after hepatic ischemia-reperfusion.
Performs important role in cell-cell binding, or in promoting phagocytosis. Specific localization of MFG-E8L(novel long form)in retinal pigment epithelium.
Title: MFG-E8 in the retina and retinal pigment epithelium of rat and mouse.
These results suggested that lactadherin could adjust intestinal DCs activity, induce CD3(+)CD4(+)CD25(+)T cell differentiation, and enhance IL-10 production.
Title: The role of the lactadherin in promoting intestinal DCs development in vivo and vitro.
arterial MFG-E8 significantly increases with aging and is a pivotal relay element within the angiotensin II/MCP-1/VSMC invasion signaling cascade.
Title: Milk fat globule protein epidermal growth factor-8: a pivotal relay element within the angiotensin II and monocyte chemoattractant protein-1 signaling cascade mediating vascular smooth muscle cells invasion.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH133320 (e-PCR)
- UniSTS:216603

Mfge8 (e-PCR), detects polymorphism
- UniSTS:546860

RH142411 (e-PCR)
- UniSTS:231658

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables integrin binding	IEA Inferred from Electronic Annotation more info
enables integrin binding	ISO Inferred from Sequence Orthology more info
enables phosphatidylethanolamine binding	ISO Inferred from Sequence Orthology more info
enables phosphatidylserine binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in angiogenesis	IEA Inferred from Electronic Annotation more info
involved_in apoptotic cell clearance	IEA Inferred from Electronic Annotation more info
involved_in apoptotic cell clearance	ISO Inferred from Sequence Orthology more info
involved_in cell adhesion	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within phagocytosis, engulfment	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within phagocytosis, recognition	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell population proliferation	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within positive regulation of phagocytosis	ISO Inferred from Sequence Orthology more info
involved_in response to estrogen	IEP Inferred from Expression Pattern more info	PubMed
involved_in single fertilization	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in acrosomal membrane	IEA Inferred from Electronic Annotation more info
located_in external side of plasma membrane	IEA Inferred from Electronic Annotation more info
located_in external side of plasma membrane	ISO Inferred from Sequence Orthology more info
located_in extracellular space	IEA Inferred from Electronic Annotation more info
located_in extracellular space	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: lactadherin

Names: MFG-E8; MFGM; O-acetyl GD3 ganglioside synthase; O-acetyl disialoganglioside synthase; O-acetyltransferase Milk fat glolbule membrane protein; O-acetyltransferase milk fat globule membrane protein; SED1; milk fat globule-EGF factor 8 protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001040186.2 → NP_001035276.1 lactadherin isoform 1 precursor

See identical proteins and their annotated locations for NP_001035276.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longer isoform (1).

Source sequence(s)

BC085754, CB322460, FQ134652, FQ151276

UniProtKB/TrEMBL

A0A0G2K506, F7F6M0, Q1PBJ1

Related

ENSRNOP00000073245.2, ENSRNOT00000079100.2

Conserved Domains (4) summary

smart00231
Location:327 → 483

FA58C; Coagulation factor 5/8 C-terminal domain, discoidin domain

cd00054
Location:26 → 60

EGF_CA; Calcium-binding EGF-like domain, present in a large number of membrane-bound and extracellular (mostly animal) proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be located at the ...

cd00057
Location:330 → 482

FA58C; Coagulation factor 5/8 C-terminal domain, discoidin domain; Cell surface-attached carbohydrate-binding domain, present in eukaryotes and assumed to have horizontally transferred to eubacterial genomes.

pfam00008
Location:68 → 106

EGF; EGF-like domain
NM_012811.3 → NP_036943.1 lactadherin isoform 2 precursor

See identical proteins and their annotated locations for NP_036943.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an in-frame coding exon compared to variant 1, and encodes a shorter isoform (2) missing an internal protein segment compared to isoform 1.

Source sequence(s)

BC085754, CB322460, FQ134652

UniProtKB/Swiss-Prot

P70490

UniProtKB/TrEMBL

A6JC45, F7F6M0

Conserved Domains (4) summary

smart00231
Location:271 → 427

FA58C; Coagulation factor 5/8 C-terminal domain, discoidin domain

cd00054
Location:26 → 60

EGF_CA; Calcium-binding EGF-like domain, present in a large number of membrane-bound and extracellular (mostly animal) proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be located at the ...

cd00057
Location:274 → 426

FA58C; Coagulation factor 5/8 C-terminal domain, discoidin domain; Cell surface-attached carbohydrate-binding domain, present in eukaryotes and assumed to have horizontally transferred to eubacterial genomes.

pfam00008
Location:68 → 106

EGF; EGF-like domain