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    Mid2 midline 2 [ Rattus norvegicus (Norway rat) ]

    Gene ID: 363502, updated on 13-Apr-2024

    Summary

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    Official Symbol
    Mid2provided by RGD
    Official Full Name
    midline 2provided by RGD
    Primary source
    RGD:1565674
    See related
    Ensembl:ENSRNOG00000060542 AllianceGenome:RGD:1565674
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Rattus norvegicus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
    Also known as
    RGD1565674
    Summary
    Predicted to enable several functions, including microtubule binding activity; protein homodimerization activity; and transcription coactivator activity. Predicted to be involved in several processes, including defense response to other organism; negative regulation of viral transcription; and positive regulation of NF-kappaB transcription factor activity. Predicted to be located in cytoplasm. Predicted to colocalize with microtubule. Human ortholog(s) of this gene implicated in non-syndromic X-linked intellectual disability 101. Orthologous to human MID2 (midline 2). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Biased expression in Lung (RPKM 111.1), Adrenal (RPKM 61.7) and 9 other tissues See more
    Orthologs
    human mouse all
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    Genomic context

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    See Mid2 in Genome Data Viewer
    Location:
    Xq33
    Exon count:
    11
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCr8 (GCF_036323735.1) X NC_086039.1 (109143057..109245257)
    RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) X NC_051356.1 (104354692..104456757)
    106 previous assembly Rnor_6.0 (GCF_000001895.5) X NC_005120.4 (112019897..112121980)

    Chromosome X - NC_086039.1Genomic Context describing neighboring genes Neighboring gene TSC22 domain family, member 3 Neighboring gene ribosomal protein L23a, pseudogene 9 Neighboring gene U6 spliceosomal RNA Neighboring gene uncharacterized LOC103694504 Neighboring gene ribosomal protein SA, pseudogene 18

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
    • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
    • BioProject: PRJNA238328
    • Publication: PMID 24510058
    • Analysis date: Mon Jun 6 17:44:12 2016

    Bibliography

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    Related articles in PubMed

    1. MID2, a homologue of the Opitz syndrome gene MID1: similarities in subcellular localization and differences in expression during development. Buchner G, et al. Hum Mol Genet, 1999 Aug. PMID 10400986
    2. MID1 and MID2 homo- and heterodimerise to tether the rapamycin-sensitive PP2A regulatory subunit, alpha 4, to microtubules: implications for the clinical variability of X-linked Opitz GBBB syndrome and other developmental disorders. Short KM, et al. BMC Cell Biol, 2002. PMID 11806752, Free PMC Article
    3. TRIM-mediated precision autophagy targets cytoplasmic regulators of innate immunity. Kimura T, et al. J Cell Biol, 2015 Sep 14. PMID 26347139, Free PMC Article
    4. TRIM protein-mediated regulation of inflammatory and innate immune signaling and its association with antiretroviral activity. Uchil PD, et al. J Virol, 2013 Jan. PMID 23077300, Free PMC Article
    5. TRIM E3 ligases interfere with early and late stages of the retroviral life cycle. Uchil PD, et al. PLoS Pathog, 2008 Feb 8. PMID 18248090, Free PMC Article

    See all (8) citations in PubMed

    GeneRIFs: Gene References Into Functions

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by RGD

    Function Evidence Code Pubs
    enables enzyme binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables identical protein binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables microtubule binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables microtubule binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables phosphoprotein binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables protein homodimerization activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables transcription coactivator activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in innate immune response ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in negative regulation of viral entry into host cell ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in negative regulation of viral transcription ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in positive regulation of DNA-templated transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in positive regulation of autophagy ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in positive regulation of canonical NF-kappaB signal transduction ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein localization to microtubule IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in protein localization to microtubule ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in suppression of viral release by host ISO
    Inferred from Sequence Orthology
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    colocalizes_with microtubule IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in microtubule ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    probable E3 ubiquitin-protein ligase MID2
    NP_001178818.1
    XP_006234309.1
    XP_006234310.1
    XP_063136223.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001191889.1 → NP_001178818.1  probable E3 ubiquitin-protein ligase MID2

      Status: PROVISIONAL

      Source sequence(s)
      JAXUCZ010000021
      UniProtKB/TrEMBL
      A0A0G2JWJ3
      Related
      ENSRNOP00000069903.2, ENSRNOT00000079841.2
      Conserved Domains (5) summary
      smart00502
      Location:239 → 364
      BBC; B-Box C-terminal domain
      smart00336
      Location:195 → 232
      BBOX; B-Box-type zinc finger
      cd00063
      Location:401 → 531
      FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
      cd00162
      Location:29 → 83
      RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...
      cd13739
      Location:536 → 705
      SPRY_PRY_TRIM1; PRY/SPRY domain of tripartite motif-binding protein 1 (TRIM1) or MID2

    RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCr8

    Genomic

    1. NC_086039.1 Reference GRCr8

      Range
      109143057..109245257
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_063280153.1 → XP_063136223.1  probable E3 ubiquitin-protein ligase MID2 isoform X2

    2. XM_006234248.5 → XP_006234310.1  probable E3 ubiquitin-protein ligase MID2 isoform X3

      Conserved Domains (5) summary
      smart00502
      Location:219 → 344
      BBC; B-Box C-terminal domain
      smart00336
      Location:175 → 212
      BBOX; B-Box-type zinc finger
      cd00063
      Location:381 → 511
      FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
      cd00162
      Location:9 → 63
      RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...
      cd13739
      Location:516 → 685
      SPRY_PRY_TRIM1; PRY/SPRY domain of tripartite motif-binding protein 1 (TRIM1) or MID2

    3. XM_006234247.5 → XP_006234309.1  probable E3 ubiquitin-protein ligase MID2 isoform X1

      See identical proteins and their annotated locations for XP_006234309.1

      UniProtKB/TrEMBL
      A6HLR4
      Conserved Domains (5) summary
      smart00502
      Location:239 → 364
      BBC; B-Box C-terminal domain
      smart00336
      Location:195 → 232
      BBOX; B-Box-type zinc finger
      cd00063
      Location:401 → 501
      FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
      cd00162
      Location:29 → 83
      RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...
      cd13739
      Location:506 → 675
      SPRY_PRY_TRIM1; PRY/SPRY domain of tripartite motif-binding protein 1 (TRIM1) or MID2
    Nucleotide Protein Strain
    Heading Accession and Version

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