Nsg1 neuronal vesicle trafficking associated 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Nsg1provided by RGD
Official Full Name: neuronal vesicle trafficking associated 1provided by RGD
Primary source: RGD:2222
See related: Ensembl:ENSRNOG00000005700 AllianceGenome:RGD:2222
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: PEP1; Bsmrb; Neep21
Summary: Enables signaling receptor binding activity. Involved in several processes, including positive regulation of receptor recycling; postsynaptic neurotransmitter receptor cycle; and spontaneous synaptic transmission. Located in several cellular components, including endosome; lysosomal lumen; and trans-Golgi network membrane. Is active in glutamatergic synapse and postsynaptic endosome. Orthologous to human NSG1 (neuronal vesicle trafficking associated 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Brain (RPKM 961.6), Adrenal (RPKM 102.4) and 1 other tissue See more
Orthologs: human mouse all
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Genomic context

Location:: 14q21

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	14	NC_086032.1 (76861079..76882822)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	14	NC_051349.1 (72648771..72670521)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	14	NC_005113.4 (77380264..77402007)

Chromosome 14 - NC_086032.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Our findings are consistent with a role for NEEP21 in facilitating vesicular transport of GluR2 between intracellular compartments and the postsynaptic plasma membrane.
Title: Neuronal enriched endosomal protein of 21 kDa colocalizes with glutamate receptor subunit GLUR2/3 at the postsynaptic membrane.
We identify the neuronal-specific endosomal protein NEEP21 (neuron-enriched endosomal protein of 21 kD) as a regulator of L1/NgCAM sorting in somatodendritic endosomes.
Title: The somatodendritic endosomal regulator NEEP21 facilitates axonal targeting of L1/NgCAM.
the essential role of NEEP21 in the recycling mechanism; this protein acts at the level of early endosomes to promote sorting of receptors toward a recycling pathway
Title: Crucial role of neuron-enriched endosomal protein of 21 kDa in sorting between degradation and recycling of internalized G-protein-coupled receptors.
Together, we conclude that NEEP21 contributes to the regulation of synaptic transmission and plasticity in slice cultures by affecting the recycling and targeting of AMPA receptors to the synapse.
Title: The endosomal protein NEEP21 regulates AMPA receptor-mediated synaptic transmission and plasticity in the hippocampus.
about 85-kDa protein kinase phosphorylates GRIP1 on serine 917. This kinase is present in NEEP21 immunocomplexes and is activated in okadaic acid-treated neurons
Title: Phosphorylation of glutamate receptor interacting protein 1 regulates surface expression of glutamate receptors.
NEEP21-GRIP1 binding is crucial for GluR2-AMPAR sorting through endosomes and their recruitment to the plasma membrane, providing a mechanism to differentially regulate AMPAR subunit cycling in internal compartments.
Title: Interactions between NEEP21, GRIP1 and GluR2 regulate sorting and recycling of the glutamate receptor subunit GluR2.
NEEP21 is a neuronal protein that is localized to the early endosomal pathway and is necessary for correct receptor recycling in neurons.
Title: Modulation of receptor cycling by neuron-enriched endosomal protein of 21 kD.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables clathrin light chain binding	IBA Inferred from Biological aspect of Ancestor more info
enables clathrin light chain binding	IEA Inferred from Electronic Annotation more info
enables ionotropic glutamate receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables signaling receptor binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in amyloid precursor protein metabolic process	ISO Inferred from Sequence Orthology more info
involved_in amyloid precursor protein metabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in apoptotic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in clathrin coat assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in clathrin coat assembly	IEA Inferred from Electronic Annotation more info
involved_in dopamine receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in endosomal transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in endosomal transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neurotransmitter receptor cycle	ISO Inferred from Sequence Orthology more info
involved_in neurotransmitter receptor cycle	ISS Inferred from Sequence or Structural Similarity more info
involved_in neurotransmitter receptor transport, endosome to postsynaptic membrane	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of receptor recycling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in postsynaptic neurotransmitter receptor cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in receptor recycling	ISO Inferred from Sequence Orthology more info
involved_in receptor recycling	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of long-term synaptic potentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spontaneous synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vesicle-mediated transport in synapse	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in Golgi cisterna membrane	IEA Inferred from Electronic Annotation more info
located_in cell projection	IEA Inferred from Electronic Annotation more info
located_in dendrite	IDA Inferred from Direct Assay more info	PubMed
located_in early endosome	IDA Inferred from Direct Assay more info	PubMed
located_in early endosome membrane	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum	ISS Inferred from Sequence or Structural Similarity more info
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
is_active_in endosome	IBA Inferred from Biological aspect of Ancestor more info
located_in endosome	IDA Inferred from Direct Assay more info	PubMed
located_in endosome	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in late endosome	IDA Inferred from Direct Assay more info	PubMed
located_in late endosome	ISO Inferred from Sequence Orthology more info
located_in lateral plasma membrane	ISO Inferred from Sequence Orthology more info
located_in lysosomal lumen	IDA Inferred from Direct Assay more info	PubMed
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in multivesicular body membrane	IEA Inferred from Electronic Annotation more info
is_active_in postsynaptic endosome	IDA Inferred from Direct Assay more info	PubMed
located_in postsynaptic endosome	IDA Inferred from Direct Assay more info	PubMed
located_in postsynaptic membrane	ISO Inferred from Sequence Orthology more info
located_in recycling endosome membrane	IDA Inferred from Direct Assay more info	PubMed
located_in somatodendritic compartment	IDA Inferred from Direct Assay more info	PubMed
located_in trans-Golgi network membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: neuronal vesicle trafficking-associated protein 1

Names: brain neuron cytoplasmic protein 1/2; brain specific mRNA b; neuron specific gene family member 1; neuron-enriched endosomal protein of 21 kDa; neuron-specific protein family member 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_024128.4 → NP_077042.2 neuronal vesicle trafficking-associated protein 1

See identical proteins and their annotated locations for NP_077042.2

Status: VALIDATED

Source sequence(s)

JAXUCZ010000014

UniProtKB/Swiss-Prot

P02683, P02684, Q6P9Y0

UniProtKB/TrEMBL

A0A8L2Q3K9, A6IJU5

Related

ENSRNOP00000097258.1, ENSRNOT00000118922.1

Conserved Domains (1) summary

pfam06387
Location:1 → 177

Calcyon; D1 dopamine receptor-interacting protein (calcyon)

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086032.1 Reference GRCr8

Range

76861079..76882822

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039091623.2 → XP_038947551.1 neuronal vesicle trafficking-associated protein 1 isoform X1

UniProtKB/Swiss-Prot

P02683, P02684, Q6P9Y0

UniProtKB/TrEMBL

A0A8L2Q3K9, A6IJU5

Conserved Domains (1) summary

pfam06387
Location:1 → 177

Calcyon; D1 dopamine receptor-interacting protein (calcyon)
XM_063272865.1 → XP_063128935.1 neuronal vesicle trafficking-associated protein 1 isoform X2