Plcd4 phospholipase C, delta 4 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Plcd4provided by MGI
Official Full Name: phospholipase C, delta 4provided by MGI
Primary source: MGI:MGI:107469
See related: Ensembl:ENSMUSG00000026173 AllianceGenome:MGI:107469
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 4921507K24Rik
Summary: Predicted to enable G-protein alpha-subunit binding activity; guanyl-nucleotide exchange factor activity; and phosphatidylinositol phospholipase C activity. Acts upstream of or within acrosome reaction. Predicted to be located in cytosol; endoplasmic reticulum; and plasma membrane. Is expressed in nervous system and testis. Orthologous to human PLCD4 (phospholipase C delta 4). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in testis adult (RPKM 22.6), cerebellum adult (RPKM 4.2) and 3 other tissues See more
Orthologs: human all
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Genomic context

Location:: 1 C4; 1 38.54 cM

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	1	NC_000067.7 (74581175..74605137)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	1	NC_000067.6 (74541975..74565977)

Chromosome 1 - NC_000067.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

We identify PLCdelta4 as a PLC isoform important in the regulation of TRPM8 both at the cellular level and in vivo
Title: Phospholipase C δ4 regulates cold sensitivity in mice.
These findings highlight a novel pathway by which nuclear PLCs affect insulin secretion and identify PPARgamma as a novel molecular target of nuclear PLCs.
Title: Nuclear PLCs affect insulin secretion by targeting PPARγ in pancreatic β cells.
PLCd4 is required for the acrosome reaction in fertilization.
Title: Physiological functions of phospholipase C delta-type.
PLCdelta(1) and PLCdelta(4) are probably differentially regulated in distinct cellular compartments by PI(4,5)P(2) and the PH domain of PLCdelta(4) does not act as a localization signal
Title: The pleckstrin homology domain of phosphoinositide-specific phospholipase Cdelta4 is not a critical determinant of the membrane localization of the enzyme.
PLCd4 has a role in the zona pellucida-induced acrosome reaction in mice
Title: Phospholipase Cdelta4: from genome structure to physiological function.
It is concluded that PLC delta4 regulates the liver regeneration in cooperation with nuclear PKC alpha and epsilon
Title: Disruption of phospholipase Cdelta4 gene modulates the liver regeneration in cooperation with nuclear protein kinase C.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (3) 1 citation

General gene information

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Markers

AI045490 (e-PCR)
- UniSTS:248396

Plcd4 (e-PCR), detects polymorphism
- UniSTS:546953

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables G-protein alpha-subunit binding	ISO Inferred from Sequence Orthology more info
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables guanyl-nucleotide exchange factor activity	ISO Inferred from Sequence Orthology more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables phosphatidylinositol phospholipase C activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphatidylinositol phospholipase C activity	ISO Inferred from Sequence Orthology more info
enables phospholipase C activity	TAS Traceable Author Statement more info	PubMed
enables phosphoric diester hydrolase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within acrosome reaction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular signal transduction	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within lipid catabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within lipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in lipid metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within phosphatidylinositol metabolic process	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within signal transduction	TAS Traceable Author Statement more info	PubMed
involved_in small GTPase-mediated signal transduction	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in intracellular anatomical structure	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4

Names: PLC-delta-4; phosphoinositide phospholipase C-delta-4

NP_001074925.1

EC 3.1.4.11

NP_683739.2

EC 3.1.4.11

XP_006495846.1

EC 3.1.4.11

XP_006495847.1

EC 3.1.4.11

XP_006495848.1

EC 3.1.4.11

XP_006495850.1

EC 3.1.4.11

XP_006495851.1

EC 3.1.4.11

XP_006495852.1

EC 3.1.4.11

XP_017174835.1

EC 3.1.4.11

XP_036018673.1

EC 3.1.4.11

XP_036018674.1

EC 3.1.4.11

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001081456.1 → NP_001074925.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4 isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter protein (isoform 1).

Source sequence(s)

AC117610

Consensus CDS

CCDS35617.1

UniProtKB/Swiss-Prot

Q8K3R3

Related

ENSMUSP00000109376.2, ENSMUST00000113747.8

Conserved Domains (8) summary

cd00275
Location:639 → 764

C2_PLC_like; C2 domain present in Phosphoinositide-specific phospholipases C (PLC)

PLN02228
Location:207 → 752

PLN02228; Phosphoinositide phospholipase C

pfam00169
Location:17 → 123

PH; PH domain

pfam09279
Location:207 → 289

EF-hand_like; Phosphoinositide-specific phospholipase C, efhand-like

pfam13499
Location:143 → 199

EF-hand_7; EF-hand domain pair

pfam14788
Location:153 → 202

EF-hand_10; EF hand

cl14615
Location:289 → 609

PI-PLCc_GDPD_SF; Catalytic domain of phosphoinositide-specific phospholipase C-like phosphodiesterases superfamily

cl17171
Location:18 → 133

PH-like; Pleckstrin homology-like domain
NM_148937.2 → NP_683739.2 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4 isoform 2

See identical proteins and their annotated locations for NP_683739.2

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR and includes an alternate in-frame exon in the coding region compared to variant 1. The resulting protein (isoform 2) is longer but has the same N- and C-termini compared to isoform 1.

Source sequence(s)

AC117610

Consensus CDS

CCDS35616.1

UniProtKB/Swiss-Prot

Q3USN9, Q6NZF7, Q8CAB1, Q8K3R3, Q9CUC1

Related

ENSMUSP00000027362.8, ENSMUST00000027362.14

Conserved Domains (4) summary

PLN02228
Location:207 → 784

PLN02228; Phosphoinositide phospholipase C

pfam14788
Location:153 → 202

EF-hand_10; EF hand

cl14615
Location:289 → 641

PI-PLCc_GDPD_SF; Catalytic domain of phosphoinositide-specific phospholipase C-like phosphodiesterases superfamily

cl17171
Location:18 → 133

PH-like; Pleckstrin homology-like domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000067.7 Reference GRCm39 C57BL/6J

Range

74581175..74605137

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006495787.5 → XP_006495850.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4 isoform X2

Conserved Domains (4) summary

PLN02228
Location:207 → 772

PLN02228; Phosphoinositide phospholipase C

cd13363
Location:18 → 133

PH_PLC_delta; Phospholipase C-delta (PLC-delta) pleckstrin homology (PH) domain

pfam14788
Location:153 → 202

EF-hand_10; EF hand

cl14615
Location:289 → 640

PI-PLCc_GDPD_SF; Catalytic domain of phosphoinositide-specific phospholipase C-like phosphodiesterases superfamily
XM_006495785.5 → XP_006495848.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4 isoform X1

See identical proteins and their annotated locations for XP_006495848.1

Conserved Domains (4) summary

PLN02228
Location:207 → 773

PLN02228; Phosphoinositide phospholipase C

cd13363
Location:18 → 133

PH_PLC_delta; Phospholipase C-delta (PLC-delta) pleckstrin homology (PH) domain

pfam14788
Location:153 → 202

EF-hand_10; EF hand

cl14615
Location:289 → 641

PI-PLCc_GDPD_SF; Catalytic domain of phosphoinositide-specific phospholipase C-like phosphodiesterases superfamily
XM_006495783.5 → XP_006495846.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4 isoform X1

See identical proteins and their annotated locations for XP_006495846.1

Conserved Domains (4) summary

PLN02228
Location:207 → 773

PLN02228; Phosphoinositide phospholipase C

cd13363
Location:18 → 133

PH_PLC_delta; Phospholipase C-delta (PLC-delta) pleckstrin homology (PH) domain

pfam14788
Location:153 → 202

EF-hand_10; EF hand

cl14615
Location:289 → 641

PI-PLCc_GDPD_SF; Catalytic domain of phosphoinositide-specific phospholipase C-like phosphodiesterases superfamily
XM_036162781.1 → XP_036018674.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4 isoform X6

Conserved Domains (4) summary

PLN02228
Location:207 → 740

PLN02228; Phosphoinositide phospholipase C

pfam14788
Location:153 → 202

EF-hand_10; EF hand

cl14615
Location:289 → 608

PI-PLCc_GDPD_SF; Catalytic domain of phosphoinositide-specific phospholipase C-like phosphodiesterases superfamily

cl17171
Location:18 → 133

PH-like; Pleckstrin homology-like domain
XM_036162780.1 → XP_036018673.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4 isoform X4

Conserved Domains (4) summary

PLN02228
Location:207 → 783

PLN02228; Phosphoinositide phospholipase C

pfam14788
Location:153 → 202

EF-hand_10; EF hand

cl14615
Location:289 → 640

PI-PLCc_GDPD_SF; Catalytic domain of phosphoinositide-specific phospholipase C-like phosphodiesterases superfamily

cl17171
Location:18 → 133

PH-like; Pleckstrin homology-like domain
XM_006495789.5 → XP_006495852.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4 isoform X5

Conserved Domains (4) summary

PLN02228
Location:207 → 741

PLN02228; Phosphoinositide phospholipase C

pfam14788
Location:153 → 202

EF-hand_10; EF hand

cl14615
Location:289 → 609

PI-PLCc_GDPD_SF; Catalytic domain of phosphoinositide-specific phospholipase C-like phosphodiesterases superfamily

cl17171
Location:18 → 133

PH-like; Pleckstrin homology-like domain
XM_006495788.5 → XP_006495851.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4 isoform X3

See identical proteins and their annotated locations for XP_006495851.1

UniProtKB/Swiss-Prot

Q3USN9, Q6NZF7, Q8CAB1, Q8K3R3, Q9CUC1

Conserved Domains (4) summary

PLN02228
Location:207 → 784

PLN02228; Phosphoinositide phospholipase C

pfam14788
Location:153 → 202

EF-hand_10; EF hand

cl14615
Location:289 → 641

PI-PLCc_GDPD_SF; Catalytic domain of phosphoinositide-specific phospholipase C-like phosphodiesterases superfamily

cl17171
Location:18 → 133

PH-like; Pleckstrin homology-like domain
XM_017319346.3 → XP_017174835.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4 isoform X7

Conserved Domains (4) summary

PLN02228
Location:207 → 751

PLN02228; Phosphoinositide phospholipase C

pfam14788
Location:153 → 202

EF-hand_10; EF hand

cl14615
Location:289 → 608

PI-PLCc_GDPD_SF; Catalytic domain of phosphoinositide-specific phospholipase C-like phosphodiesterases superfamily

cl17171
Location:18 → 133

PH-like; Pleckstrin homology-like domain
XM_006495784.5 → XP_006495847.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4 isoform X1

See identical proteins and their annotated locations for XP_006495847.1

Conserved Domains (4) summary

PLN02228
Location:207 → 773

PLN02228; Phosphoinositide phospholipase C

cd13363
Location:18 → 133

PH_PLC_delta; Phospholipase C-delta (PLC-delta) pleckstrin homology (PH) domain

pfam14788
Location:153 → 202

EF-hand_10; EF hand

cl14615
Location:289 → 641

PI-PLCc_GDPD_SF; Catalytic domain of phosphoinositide-specific phospholipase C-like phosphodiesterases superfamily