BFA1 Bfa1p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: BFA1
Gene description: Bfa1p
Primary source: FungiDB:YJR053W
Locus tag: YJR053W
See related: SGD:S000003814; AllianceGenome:SGD:S000003814
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: IBD1
Summary: Enables GDP-dissociation inhibitor activity. Contributes to GTPase activator activity. Involved in mitotic spindle orientation checkpoint signaling. Located in spindle pole body. Part of Bfa1-Bub2 complex. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: X

Exon count:: 1

Sequence:: Chromosome: X; NC_001142.9 (534027..535751)

Chromosome X - NC_001142.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The N-Terminal Domain of Bfa1 Coordinates Mitotic Exit Independent of GAP Activity in Saccharomyces cerevisiae.
Title: The N-Terminal Domain of Bfa1 Coordinates Mitotic Exit Independent of GAP Activity in Saccharomyces cerevisiae.
We conclude that Bfa1 functions to both inhibit and activate late mitotic events.
Title: Budding Yeast BFA1 Has Multiple Positive Roles in Directing Late Mitotic Events.
Tem1 activity is tightly controlled in mitosis by Bub2 and Bfa1 and is also regulated by the spindle orientation checkpoint that monitors the correct alignment of the mitotic spindle with the mother-daughter axis. Here is described the purification of Tem1, Bfa1, and Bub2 and a detailed radioactive filter-binding assay to study the nucleotide binding properties of Tem1 and the role of its regulators Bfa1 and Bub2.
Title: In Vitro Analysis of Tem1 GTPase Activity and Regulation by the Bfa1/Bub2 GAP.
Bmh1 is a spindle position checkpoint (SPOC) component that breaks Bfa1 centrosome asymmetry upon SPOC activation.
Title: The 14-3-3 protein Bmh1 functions in the spindle position checkpoint by breaking Bfa1 asymmetry at yeast centrosomes.
Cdc5 induces the asymmetric distribution of Bfa1 to the bud-directed spindle pole body independently of Bfa1 GAP activity at anaphase.
Title: Cdc5-dependent asymmetric localization of bfa1 fine-tunes timely mitotic exit.
A link is confirmed between the subcellular localization pattern of Bfa1 at the spindle pole bodies with cell cycle progression.
Title: Lte1 contributes to Bfa1 localization rather than stimulating nucleotide exchange by Tem1.
there is a GAP-independent surveillance mechanism of Bfa1/Bub2, which, together with the GTP/GDP switch of Tem1, may be required for the genomic stability of cells with misaligned spindles
Title: Different levels of Bfa1/Bub2 GAP activity are required to prevent mitotic exit of budding yeast depending on the type of perturbations.
Results suggest that the GTPase-activating activity of Bfa1p-Bub2p is responsible for the mitotic arrest caused by spindle damage and Bfa1p overproduction.
Title: The study of Bfa1p(E438K) suggests that Bfa1 control the mitotic exit network in different mechanisms depending on different checkpoint-activating signals.
Bfa1 disappearance from the mother bound spindle pole body at the onset of anaphase, along with Cdc5 function, helps prompt Tem1 activation in telophase.
Title: Disappearance of the budding yeast Bub2-Bfa1 complex from the mother-bound spindle pole contributes to mitotic exit.
various checkpoint signals are transmitted to the C-terminal domain of Bfa1 (Bfa1-D8(391-574)) and Bfa1p localization to spindle pole body is necessary but not sufficient to regulate mitotic exit in response to various checkpoint signals
Title: The C-terminus of Bfa1p in budding yeast is essential to induce mitotic arrest in response to diverse checkpoint-activating signals.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables GDP-dissociation inhibitor activity	IDA Inferred from Direct Assay more info	PubMed
enables GTPase activator activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in mitotic spindle orientation checkpoint signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic spindle orientation checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of exit from mitosis	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of exit from mitosis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Bfa1-Bub2 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Bfa1-Bub2 complex	IDA Inferred from Direct Assay more info	PubMed
part_of Bfa1-Bub2 complex	IEA Inferred from Electronic Annotation more info
part_of Bfa1-Bub2 complex	IPI Inferred from Physical Interaction more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
is_active_in mitotic spindle pole body	IBA Inferred from Biological aspect of Ancestor more info
located_in spindle pole	IEA Inferred from Electronic Annotation more info
located_in spindle pole body	IDA Inferred from Direct Assay more info	PubMed
located_in spindle pole body	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Bfa1p

NP_012587.3

Subunit of a two-component GTPase-activating protein, Bfa1p-Bub2p; contributes to GAP activity, inactivating Tem1 by stimulating GTP hydrolysis following damage or misalignment of the mitotic spindle; functions as a guanine-nucleotide exchange inhibitor (GDI) for Tem1p; involved in multiple cell cycle checkpoint pathways that control mitotic exit; required when telomeres are damaged, but not for all types of chromosomal DNA damage; phosphorylated by the Polo-like kinase Cdc5p