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    tonB Ton complex subunit TonB [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945843, updated on 6-Jun-2024

    Summary

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    Gene symbol
    tonB
    Gene description
    Ton complex subunit TonB
    Primary source
    ASAP:ABE-0004196
    Locus tag
    b1252
    See related
    ECOCYC:EG11012
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1246; exbA; T1rec
    Summary
    Periplasmic TonB is attached to the cytoplasmic membrane by a signal anchor and interacts with outer membrane proteins, thus TonB spans the periplasmic space. [More information is available at EcoGene: EG11012]. TonB is a cytoplasmic membrane protein which transduces the proton motive force (pmf) of the cytoplasmic membrane to the outer membrane active transporters thus providing the energy source required for the import of iron-siderophore complexes and vitamin B12 across the outer membrane . [More information is available at EcoCyc: EG11012].
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    Genomic context

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    Sequence:
    NC_000913.3 (1311089..1311808)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene K(+) channel Kch Neighboring gene protein YciI Neighboring gene acyl-CoA thioesterase YciA Neighboring gene inner membrane protein

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. From Homodimer to Heterodimer and Back: Elucidating the TonB Energy Transduction Cycle. Gresock MG, et al. J Bacteriol, 2015 Nov. PMID 26283773, Free PMC Article
    2. Avian pathogenic Escherichia coli ΔtonB mutants are safe and protective live-attenuated vaccine candidates. Holden KM, et al. Vet Microbiol, 2014 Oct 10. PMID 25205199
    3. Monomeric TonB and the Ton box are required for the formation of a high-affinity transporter-TonB complex. Freed DM, et al. Biochemistry, 2013 Apr 16. PMID 23517233, Free PMC Article
    4. TonB is essential for virulence in avian pathogenic Escherichia coli. Holden KM, et al. Comp Immunol Microbiol Infect Dis, 2012 Mar. PMID 22237011
    5. Taking the Escherichia coli TonB transmembrane domain "offline"? Nonprotonatable Asn substitutes fully for TonB His20. Swayne C, et al. J Bacteriol, 2011 Aug. PMID 21665976, Free PMC Article

    See all (128) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. HDX-MS performed on BtuB in E. coli outer membranes delineates the luminal domain's allostery and unfolding upon B12 and TonB binding.
      Title: HDX-MS performed on BtuB in E. coli outer membranes delineates the luminal domain's allostery and unfolding upon B12 and TonB binding.
    2. TonB and FepA display confined mobility within Escherichia coli cell and inner membranes.
      Title: Confined Mobility of TonB and FepA in Escherichia coli Membranes.
    3. The authors found numerous specific sites of FepA interaction with TonB on the periplasmic face of the FepA cork in addition to the TonB box.
      Title: Going Outside the TonB Box: Identification of Novel FepA-TonB Interactions In Vivo.
    4. The TonB system embodies a novel means of active transport across the outer membrane for nutrients.
      Title: From Homodimer to Heterodimer and Back: Elucidating the TonB Energy Transduction Cycle.
    5. ExbB-ExbD-TonB form complex in molar ratios of 4:1:1.
      Title: Membrane Protein Complex ExbB4-ExbD1-TonB1 from Escherichia coli Demonstrates Conformational Plasticity.
    6. Avian pathogenic Escherichia coli DeltatonB mutants are safe and protective live-attenuated vaccine candidates.
      Title: Avian pathogenic Escherichia coli ΔtonB mutants are safe and protective live-attenuated vaccine candidates.
    7. This study has demonstrated that TonB is essential for virulence in avian pathogenic Escherichia coli.
      Title: TonB is essential for virulence in avian pathogenic Escherichia coli.
    8. These observations supported the hypothesis that ExbD couples TonB to the protonmotive force, with concomitant transitions of ExbD and TonB periplasmic domains from unenergized to energized heterodimers.
      Title: ExbD mutants define initial stages in TonB energization.
    9. ExbD-TonB interactions required ExbD transmembrane domain residue D25.
      Title: The same periplasmic ExbD residues mediate in vivo interactions between ExbD homodimers and ExbD-TonB heterodimers.
    10. ExbB(6) and ExbB(6)ExbD(1) are subcomplexes on which the final complex including TonB is assembled
      Title: Oligomeric structure of ExbB and ExbB-ExbD isolated from Escherichia coli as revealed by LILBID mass spectrometry.
    Submit: New GeneRIF Correction See all GeneRIFs (29)

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables energy transducer activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables energy transducer activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables energy transducer activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables energy transducer activity TAS
    Traceable Author Statement
    more info
    		 PubMed 
    enables protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein domain specific binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in bacteriocin transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within cobalamin transport IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within cobalamin transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in cobalamin transport NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    acts_upstream_of_or_within colicin transport TAS
    Traceable Author Statement
    more info
    		 PubMed 
    involved_in intracellular iron ion homeostasis NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in intracellular monoatomic cation homeostasis NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in protein transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within receptor-mediated bacteriophage irreversible attachment to host cell TAS
    Traceable Author Statement
    more info
    		 PubMed 
    acts_upstream_of_or_within siderophore transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in siderophore transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cell envelope IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cell outer membrane NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    located_in membrane NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    located_in outer membrane-bounded periplasmic space IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in outer membrane-bounded periplasmic space IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of plasma membrane protein complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of plasma membrane protein complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of transmembrane transporter complex NAS
    Non-traceable Author Statement
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    Ton complex subunit TonB

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1311089..1311808
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415768.1 Ton complex subunit TonB [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415768.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A0A0FQP4, A0A1X3JK35
      Conserved Domains (1) summary
      PRK10819
      Location:1 → 239
      PRK10819; transport protein TonB; Provisional
    Nucleotide Protein
    Heading Accession and Version

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